Table S1: Genes regulated > 2-fold by TEL-AML1 in EML1 cells, identified by gene expression
profiling. TA-B0 refers to genes regulated prior to any treatment, TA-B7 refers to genes
regulated after 7 days of IL7 + FLT3L treatment. Red or green cells indicate increased or
decreased expression, respectively.
Table S2: Functional classification of genes that are regulated by TEL-AML1 in EML1 cells. The
most significant functions that emerged from GSEA analysis are shown.
Figure S1: Analysis of B-lymphoid differentiation of EML1 cells. (A) FACS analysis of EML1 cells at
different time points of B cell differentiation shows a progressive increase in the expression levels
of B cell specific markers. (B0, B5, B7, B15 respectively indicate 0, 5, 7 and 15 days of IL7 + FLT3L
treatment – see Materials and Methods). (B) GSEA comparison between gene expression profiles
at day B0 and day B7 revealed an enrichment of B cell specific mRNAs. Results for two
experimental replicas are shown. (C) Genes involved in B cell functions are upregulated during Blymphoid differentiation of EML1 cells.
Figure S2: DotPlot analysis of B cell differentiation of EML1-ev and EML1-TA: FACS analysis of
empty vector-infected cells (ev) and TEL-AML1 expressing cells (TA) at B0, B5 and B7. IL7R, CD19, B220 and
IgM expression were considered.
Figure S3: DotPlot analysis of B cell differentiation of Lin-ev and Lin-TA: FACS analysis of IL7R and
CD19 expression as described in Fig.1C
Figure S4: DotPlot analysis of B cell differentiation of Lin-ev and Lin-TA: FACS analysis of IL7R and
CD19 expression as described in Fig.1D
Figure S5: DotPlot analysis of B cell differentiation EML-ev and EML-TA: FACS analysis of IFNR
expression as described in Fig.2C
Figure S6: DotPlot analysis of B cell differentiation of Lin-ev +/- siRNA IRF3: FACS analysis of IL7R
and CD19 expression as described in Fig.5C
Figure S7: DotPlot analysis of B cell differentiation of EML-ev and EML-TA after mTOR pathway
interference: FACS analysis of IL7R and CD19 expression as described in Fig.7C
Figure S8: DotPlot analysis of B cell differentiation of REH: FACS analysis of IgM expression as
described in Fig.8D
Figure S9: DotPlot analysis of B cell differentiation of SUP-B15: FACS analysis of IgM expression as
described in Fig.8D