A Report on VM 197-Internship II/ VM 201-Case Report Presented to the faculty of College of Veterinary Medicine, Benguet State University, La Trinidad, Benguet In partial fulfilment of the requirements For the degree DOCTOR OF VETERINARY MEDICINE By MARIA CRISTINA C.BELTRAN VENUS O. CASAO CRISTA M. IGUID CLARENCE N. PANAWAN April 2012 Swine Station: Luz Farms, Inc Pinagsibaan, Rosario, Batangas On April 10-30, 2011 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE FARM A. Farm History and Present Status Luz Farms, Inc established in 1981 provided the best swine genetics and breeding stocks. It integrated production facilities on a 30-hectare property in Barangay Pinagsibaan, Rosario, Batangas. Since then, the farm has a total sow level of 1, 610 capable of producing F1 gilts per year. JSR genetics has brought together 3 of the world’s leading genetic houses, namely: JSR Healthbred, JSR Newsham, and Cotswold Pig Development Company in synergistic new combination which provides pig producers with an extensive range of sire and dam line products, artificial insemination (AI) products and services, expert technical support and online customer technical services. LFIs stock came from HY-Farm, Australia, which was LFI’s network established in 1986 and with 3 farms: JSR Health Breed, UK, Norsvin, Norway and Waratah, New Zealand. Luz Farms, Inc is one of the farms accredited by Bureau of Animal Industry because of its feasible and efficient farm production. An artificial insemination center of the farm is built in the municipality to give backyard raisers easy access to the semen of selected boars. B. Farm Lay-out Luz Farms, Inc is approximately 20 km from Lipa City and one km away from the national road. It is accessible by land transportation since its topography is generally flat. The farm highly suitable for swine raising is fenced with hollow blocks. Its total land area is 30 hectares. C. Physical Resources In designing a piggery house, the first requirement is to protect the animals from direct sunlight. Luz Farm, Inc has a monitor type of roofing. The roofs are designed in a way that they reduce the ambient heat load reaching the animals and provide an efficient removal of animal’s body heat. Water for all production buildings is connected to three huge water tanks. Each building is provided with medication tank, the breeding, farrowing and nursery buildings are equipped with ventilating fans to control humidity. Pens are made of GI pipes and steel bars. Doors are durable and provided with security tight locks. The farm has a house where some farm personnel, field practitioners and buyers reside. It has a total of 42 production buildings divided into 2 areas, Area 1 and 2 where the pigs are found. II. FARM SECTIONS A. Vaccination and Necropsy Section Activities: Vaccination of piglets Vaccination of weaners, gilts and boars Necropsy of deceased pigs Facilities: Store Room 1. This room serves as storage for feeds of the animals. Store Room 2. This room serves as storage for vaccines and medicines of the animals. An overseer is assigned to keep watch of the distributing and inventory of the products. Hospital building. This building isolated from the rest of the farm buildings serves to cater pigs under medication and observation. This is provided with canvass curtains, medication tanks, nipple type waterers, feeding troughs and medicines. Necropsy sheds. These provide areas for necropsy. B. Breeding Section Activities: Heat detection. Heat is the time that the female accepts the male for mating. During farm breeding time, gilts and sows are keenly observed if they give signs of estrus and therefore ready to be bred. The surest indication of a sow in heat is its acceptance of the boar. The producer’s ability to detect estrus is improved if the sow’s behaviour is observed in the presence of a boar. Several signs of estrus are swelling and reddening of the vulva, loss of appetite, mucus discharge, mounting other animals and restlessness as given by PCCARD (2006). Techniques included in heat detection are haunch back pressure, riding-the-back test, semen-on-snout test and teaser boar method. Semen collection and processing. Collection of semen from selected boars is done in this section. The collection pen is designed to have rubberized flooring for firm gripping of the boar’s hoofs during mounting of dummy. With gloved hand gently stroke the boar’s genitals then holding the boar’s penis at the tip, pull on the penis and grip it firmly. The first gel-like ejaculate is thrown while the creamy part is collected in a clean cup with paper. After further evaluation in the AI laboratory, the semen is diluted, extended, sealed and stored in the fridge at 16-18°C. Semen evaluation. The collected semen is brought here for evaluation. Motility, concentration and volume are recorded. For motility evaluation, a drop of sample is observed under low power objective magnification. For concentration, the spermiodensimeter is used. The semen is measured through the use of a beaker. Artificial insemination. Artificial insemination, a breeding process wherein the boar’s semen is introduced into the sow’s reproductive tract by way of catheter, becomes the most practical means of breeding in the piggery farm because of the many advantages it offers such as increased number of sows that are serviced, elimination of mating accidents, avoidance of the spread of sexually transmitted diseases, allowing smaller raisers to get the best line for establishing a farm and allowing breeding possible even in distant farms because of ease of transportation of semen. Pregnancy test. A mobile sonometer is used for detection of pregnancy. Examination is done at 25 days after insemination. Feeding Record keeping Boar management. Reproductive performance of the herd depended on proper care and management. Breeding boars are allowed to breed sows and gilts at 8 months of age depending on their performance. Senior boars were used for semen collection that is done twice or thrice daily in a week. On the other hand, junior boars are only collected once a week. Management of pregnant gilts and sows. Inseminated sows and gilts are housed in individually for further observation of signs of pregnancy. Gilts and sows that failed to exhibit such signs after 17-24 days are re-bred. Feeding of pregnant sows and gilts are regulated to prevent them from getting obese and avoid any embryonic abnormality. Ventilation fans are provided to offset high humidity build-up in their pens. Fresh water is made available through automatic nipple type waterers. Facilities: Buildings. There are 6 buildings in this section occupied by gestating sows, dry sows and teaser boars. The houses have monitor type of roofing and are equipped with ventilating fans to minimize the accumulation of heat and for better air distribution. Pens are provided with automatic nipple type waterers. AI Laboratory. The farm has only one laboratory provided with all the equipments such as microscope, fridge for semen, measuring pitcher, hot water bath, electric sealer, spermiodensimeter, pregnancy test detector/scanner, catheter, glass slides, and semen tubes. C. Farrowing Section Activities: Assisting farrowing sows Cleaning farrowing pens Weighing piglets Needle teeth cutting Umbilical cord cutting Ear notching Tail docking Iron administration Castration D. Performance Testing Unit (PTU) PTU is further divided as the following: 1. Performance Testing Unit Activities: Semen collection Training of junior boars Teasing of gilt feeding and bathing pigs Backfat thickness testing Weighing of pigs Medication Record-keeping 2. Growing Section Activities: Cleaning of pens and alley Bathing of pigs Feeding medication Weighing of pigs 3. Nursery Section Activities: Feeding of piglets Medication Cleaning and washing of pens and alley Record-keeping Loading of pigs on the cart Fig. 1 – 2. Semen collection and iron supplementation. Fig. 3. Weighing and testing for backfat thickness of the gilts Fig. 4. Taildocking done on a <14-old-old piglet. Poultry Station: Dalamax Poultry Farm And Adela’s Poultry Farm Gueset, Bugallon, Pangasinan On May 01-20, 2011 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE FARM A. Farm Description Dalamax and Adela’s Poultry Farms are contractual broiler growers. II. ACTIVITIES AND FACILITIES IN THE FARM A. Feeding and Watering Sacks of feeds are stocked in a room wherein feeds are released in a bin that supplies all the feeders with the use of an automated dispenser. A feed sensor connected to the feeders is used to help regulate the dispensing of feeds. The farms have the following as feeding guide: Booster feed – first week Grower – second to third week Finisher – fourth week A Dosatron® water regulator is used in the farm connected to the automated waterers. B. Temperature Regulation Special equipment is used to regulate the large exhaust fans installed at the end of the buildings. Cooling pads are also installed at the sides of the building to help regulate the temperature inside the building. During summer, fans are set according to the following: REQUIRED TEMPERATURE (ºC) DAY REQUIRED FAN 1-3 none 35-38 4 1/20m3 32 2 if 32 ºC and above 5-6 32 7-9 3-4 fans 31 10-15 4-6 fans 30 16-20 6-8 fans 29 21-25 With cooling pads 28 25-30 With cooling pads 27 31-35 With cooling pads 26 During rainy season, fans are set accordingly: REQUIRED TEMPERATURE (ºC) DAY REQUIRED FAN 0 none 34 3 0-2 33 4 3-4 32.6 7 4-6 (1min on;3min off) 31 10 4-6 30.8 16 Auto-cooling 29.2 18 Auto-cooling 28.2 20 Auto-cooling 27.4 25 Auto-cooling 26.2 28 Auto-cooling 25.6 C. Medication and Supplementation Birds observed to have abnormalities are culled. Multivitamins, amino acids and electrolytes are given for supplementation. D. Harvest Target weight for a 33-day broiler is 1.8 kilograms and above. At harvest days, the broilers are loaded to the trucks to be delivered to the dressing plants. Fig. 5 – 6. The cooling pad and the control panel for the fans and feeders. Fig. 7. The exhaust fans installed at the end of the building. Fig. 8 – 9. The broilers at feeding time and the automated water dispenser. Small Animal Practice Station: BSU- Veterinary Hospital La Trinidad, Benguet On 07-19 November 2011 16-21 January 2012 20-25 February 2012 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE ESTABLISHMENT A. Hospital History and Present Status Benguet State University – College of Veterinary Medicine had its humble beginning way back 1975 when Dr. Basito Cotiw-an proposed the offering of a new degree, Bachelor of Science in Animal Technology or BSAT. It was on June 15, 1977 that BSAT was offered under the Veterinary Technology Division of the Department of Veterinary and Animal Sciences of the College of Agriculture through the Memorandum No. 2 Series of 1977 of the MSAC Administration. On June 1978, Dr. Cotiw-an became the Department Chairman wherein the department was subdivided into Veterinary Sciences headed by Dr. Reynaldo Galban, and Animal Sciences headed by Professor Sydney Moresto. Four years after the offering of BSAT, the first batch of BSAT students graduated on March 1979. In 1982, Dr. Domingo Casiwan took over as the Department Chairman. It was under his administration that the integrated ladder type of BSAT-DVM curriculum was approved through Resolution No. 7 Series of 1982. It was on 1983 that the Veterinary Sciences became a separate department from Animal Sciences and the assigned chairman was Dr. Cotiw-an. The Department of Veterinary Sciences became a separate college of the university, the College of Veterinary Medicine, in 1984 with Dr. Cotiw-an as the Dean, who was taken over by Dr. Abraham Patacsil. On February of the same year, the Technical Panel on Agricultural Education (TPAE) evaluated the Doctor of Veterinary Medicine program. The College of Veterinary Medicine was re-evaluated and was recommended to be phased out on April 5, 1987. On 1988, Dr. Lucio Victor, the University President improved the college facilities including the construction of the animal clinic and the three-storey building. On July 4 of the same year, Dr. Reynaldo Galban became the College Dean. From July 14, 1990 to March 31, 1994, Dr. Anatalia Castrence took over as the College Dean and it was under her administration that the college was given a rate of 81.19% by the TPAE re-evaluation. On March 1997, six college departments were constructed namely: Anatomy, Physiology and Pharmacology, Pathology and Public Health, Microbiology, Medicine and Surgery, and Zootechnics. On April 14, 1998, the college was soaring high to becoming as a Center of Excellence for Veterinary Education in Northern Luzon. Dr. Ruth Diego became the Dean on June 10, 1999-2003. Dr. Anatalia Castrence took over again as the Dean on June 2003. The following year up to the present, Dr. Joseph Dianso was assigned the Dean of College with Dr. Louwell Ogbinar as the Resident Veterinarian and Dr. Jocelyn Runas as the Director of the Animal Hospital. On the second semester of school year 2010-2011, the Animal Hospital was retiled, re-painted and totally renovated. On June 2009, Dr. Maricel West took over as the Resident Veterinarian and had its leave on April 2011. Dr. Allan Labotan was hired as reliever for the position and Dr. Melly Ann Salic-o was later hired on June of the same year and recently as the temporary Resident Veterinarian. II. HOSPITAL SECTIONS A. Information Activities: Anamnesis and physical examination of patient Reception of clients Filing up of records Facilities: Information desk. This serves for reception of clients and filling up of records. Under desks contain several forms such as laboratory request, necropsy, patient medical record, surgery, anesthesia/sedation, routing, vaccination/deworming and euthanasia forms. Seats are provided for the comfort of clients while waiting. Vaccination and/or deworming table. This caters to patients for vaccination and deworming. There are two weighing scales – the smaller for the small patients and the bigger for the big ones. Examination table. This caters to patients undergoing anamnesis and physical examination. Thermometers, alcohol, cotton and tissues are fixed on top of the table for use during physical examination. B. Pharmacy Activities: Drug dispensation Selling of products such as cages, feeds, chokers, chains, etc Inventory of drugs and other products Facilities: Cabinets. Glass-covered cabinets are used for display and easy access of drugs. Refrigerator. This is in use for storage of drugs, vaccines, antitoxins, ice packs, test kits and food for in-patients. C. Laboratory Activities: Fecalysis Urinalysis Hematology Seminal fluid examination Skin scraping and pus examination Vaginal cytology Sterilization of sampling bottles, syringes and IV lines for fecalysis Facilities: Wooden cabinets. These contain the reagents and solutions needed for fecalysis, urinalysis, fungal analysis and hematology. Underdesks are forms of fecalysis, urinalysis, hematology, skin scraping and pus examination, vaginal cytology and seminal fluid examination Drawers. These contain the pipettes, cell counter, strips for urinalysis, glass slides, cover slips, tongue depressors, sampling bottles, syringes for sample collection, cotton swabs, capillary tubes, and sterilized syringes with improvised IV lines for fecalysis. Marble lavatories. On top are electron microscopes for viewing slides, staining boxes, testtube brushes, soap, sponges, disinfectant, left-over IV fluids/distilled water for fecalysis, draining platforms, microhematocrit centrifuge and reader , tube centrifuge and water bath. Refrigerator. This is for the storage of ice packs and miscellaneous materials. Nebulizer/humidifier. For patients afflicted with respiratory diseases that need nebulisation or humidification. D. Non-infectious treatment Activities: patients Treatment of out-patients Treatment of patients with non-contagious diseases and post-surgical Facilities: Treatment table. This stainless long table caters to cages of patients for elevation, easy access to patients and easy elimination of excreta. Feeders and drinkers. These are for feeding and drinking. Lavatory. This is equipped with faucet, soap and disinfectant for washing of feeders and drinkers of patients. IV drip stand. Push cart. This contains gloves, masks, newspapers, bulbs, extension wires, muzzles, leashes, collars, gauzed cottons, iodine, alcohol and feeds for the patients. Hot packs and incandescent bulbs with extension wires. These are for the provision of warmth and therapy to patients. E. Surgery Activities: Minor and major surgery/ies Packing and sterilization of materials for surgery instruments/equipments, apparels and other Cleaning and disinfection of area Facilities: Washing performance of surgery. and dressing room. This is for scrubbing and garbing prior to Autoclave apparatus. This is for sterilizing surgical instruments, gauzes, drapes, masks and gowns. Stainless surgical table. It is a hydraulic operated surgical table. Wooden cabinets. This is for the storage of surgical supplies materials such as splints, dressings and plaster. operation. Crash cart. This contains emergency drugs. Oxygen tank and mask. Push cart. This contains the surgical instruments and casting that are used for the F. X-ray Activities: X-ray examination Ultrasound examination Facilities: Dark room. A well-equipped room needed for the development of films. X-ray machine. This a tool for taking radiographs. Ultrasound apparatus. Positioning equipments. These are table and trough. G. Infectious Ward Activities: Treatment of patients with infectious diseases Facilities: Cages with pans. These cater for admitted patients. Feeders and drinkers. These are for feeding and drinking. Lavatory. This is equipped with faucet, soap and disinfectant for washing of feeders and drinkers of patients. IV drip stand. Push cart. This contains gloves, masks, newspapers, bulbs, extension wires, muzzles, leashes, collars, gauzed cottons, iodine, alcohol and feeds for the patients. Hot packs and incandescent bulbs with extension wires. These are for the provision of warmth and therapy to patients. H. Extension Activities: patients Home services such as castration, vaccination, deworming and fetching of Necropsy Laundry Disinfection of used cages Update on the health condition of BSU-CVM animals and medication of animals that need one cadavers Interment of contaminated syringes, blades, IV lines and needles and Facilities: patients. Necropsy room. This caters to performance of necropsies of expired Laundry area. This is for laundry and disinfection of used cages. Graveyard. III. CASES A. Infectious Patient Information: Spark, a seven-month-old, black tan male Dachshund weighing 2.3kilograms. Anamnesis: It was admitted due to inappetence and emesis. It has no vaccination or deworming record. Physical examination: Laboratory Examination: Immunocomb® test kit – positive for parvovirosis Charting: 08 November. The line of treatment was as follows: Biodyl® – 1cc IV Canglob P® – 2.9cc IV Metoclopramide – 0.37cc IV Metronidazole – 7cc IV b.i.d Marbocyl® – 0.73cc IV Biodyl® is a B-complex vitamins (B1 – thiamine, B2 – riboflavin, B3 – niacin, B6 – pyridoxine, B12 – cyanocobalamin) preparation. It is an adjunct therapy and as support in animals receiving antibiotics or chemotherapeutics in cases of weakness, recovery from infectious, parasitic infestation, nervous disturbances and avitaminosis. Canglob P®’s active ingredients are immunoglobulin anti parvivirosis canis NLT 1024 HIU and thiomersal NMT 0.1mg; PBS solution ad 1ml. Metoclopramide has two pharmacologic classifications, as anti-emetic and prokinetic. It is indicated as anti-emetic for intractable emesis caused by blood-borne toxins, nausea and vomition associated with delayed gastric-emptying, gastroesophageal reflux, reflux gastritis and peptic ulceration. It is indicated as a prokinetic for gastric dilatation and/or volvulus, post-operative ileus, gastric ulcer and idiopathic gastroparesis. Metronidazole that is a nitroimidazole is classified as a miscellaneous antibiotic. It is used commonly to treat protozoal infections and anaerobic bacterial infections. It also has anti-inflammatory effects in the bowel. Metronidazole is bactericidal; it kills bacterial microorganisms by disrupting their DNA. It is absorbed rapidly from the GI tract, metabolized by the liver and excreted in the urine and the feces. Marbocyl® is a marbofloxacin, a fluoroquinolone-classified antibiotic not commonly used for minor infections. Choice of fluid was crystalloid – an isotonic 5% dextrose lactated Ringer’s solution of which has the following formulation: 5g of dextrose monohydrate 600mg of sodium lactate anhydrous 30mg of potassium chloride 20mg of calcium, calcium chloride dehydrate Percentage of dehydration was estimated using the table below: Estimated Dehydration (%) Physical Examination Findings <5 History of fluid loss but no findings upon on physical examination 5 Dry oral mucous membranes; no panting or tachycardia 7 (mild) Decreased skin turgor, dry oral mucous membrane, slightly tachycardia, normal pulse pressure 10 (moderate) Marked degree of decreased skin turgor, dry oral mucous membrane, tachycardia, increased pulse 12 (severe) Marked loss of skin turgor, dry oral mucous membrane, presence of significant signs of shock Fluid therapy was computed by the following: Replacement of lost interstitial volume (rehydration) % dehydration x kg BW 5% x 2.3kg = 115ml Maintenance fluids for normal homeostasis Constants: 40ml/kg/day for larger animals 60ml/kg/day for smaller animals Rehydration rate + 40 or 60ml/kg/day 115ml + 60 = 175ml/day On-going or increased fluid losses (estimated by measuring urine, fecal output, nasogastric tube suction, vomitus volume and insensible losses increased with fever or higher metabolic demands at a range rate of 15-20ml/kg/day) 175ml + 15ml = 190ml/day or 24hours = 7.92ml/hr 60min = 0.13 x 15 drops (microset drops = 1ml) = 1.98gtt/min 09 November. Same line of treatment was administered except for antiemetic. 10 November. Same line of treatment was administered except for antiemetic. 11 November. It was brought outside for sun-basking and walked for exercise and evacuation of urine/feces. It was fed with mashed Pyramid Hill® in lukewarm water. 12 November. It was walked outside after it ate mashed Pyramid Hill®. It urinated but no stool was observed. Take-home medicines including 1 bottle of molasses, an additional of 3 metronidazole tablets to complete the seven-day dosage of antimicrobial therapy, 7 capsules of Anti-anemia® were readied. Anti-anemia® contains ginseng and astralagus extract. Intern: Crista M. Iguid Patient Information: Fire, a three-month-old yellow mongrel weighing 6.6kg. Fig. 10. Fire on its third day. Anamnesis: It was admitted with its inappetence. Physical Examination: <2cm in diameter anorexic area on calvarium and a bit of sluggish general appearance Laboratory Examination: Fecalysis - No ova seen Skin scraping - Negative for parasite Recommendation: For admission to be observed further being suspected of having same infection as with the admitted but then discharged littermate. Charting: 11 November. Choice of fluid for intravenous infusion was 5% dextrose lactated Ringer’s solution with the total fluid of 405ml/kg/day perfused at 4.21 drops per minute. Fluid therapy was computed by the following: Replacement of lost interstitial volume (rehydration) % dehydration x kg BW x total body water (0.6) 5% x 6.6kg x 1 = 330ml Maintenance fluids for normal homeostasis Constants: 40ml/kg/day for larger animals 60ml/kg/day for smaller animals Rehydration rate + 40 or 60ml/kg/day 330ml + 60 = 390ml/day On-going or increased fluid losses (estimated by measuring urine, fecal output, nasogastric tube suction, vomitus volume and insensible losses increased with fever or higher metabolic demands at a range rate of 15-20ml/kg/day) 390ml + 15ml = 405ml/day or 24hours = 16.88ml/hr 60min = 0.28 x 15 drops (microset drops = 1ml) = 4.21gtt/min 12 November. Same line of treatment was as follows: Biodyl® – 0.7cc IV Canglob® – 2.9cc IV Metronidazole – 19.8cc IV b.id. 13 November. Same line of treatment was done. The pup was brought outside for basking and exercise. Its feces were bloody and fishy liquid. It was fed then medicated. Its second dose of Canglob P® was administered. 14 November. Same line of treatment was followed since its first day but Canglob P® was excluded. It displayed tenesmus though feces were hard to soft to unformed. It frequently rubbed against the ground its anus. It ate a lot of mashed Pyramid Hill®. It was brought home with each bottle of metronidazole, LC-vit® and molasses. (Intern: Crista Iguid) Patient Information: Bambi, a three-to-four-month-old 5.5kg brown with black fur bitch Fig. 11. Bambi being infused with intravenous fluid. Anamnesis: It had been anorexic, chocolate-like diarrhea and vomiting for about four days. Alaxan® was administered prior to check-up. Its diet had been rice and table scraps. Physical Examination: Initial temperature was 39ºC. It was lethargic, had tented skin, pale mucous membrane, ocular mucoid discharge, dirty ears, dry nasal plane, no borborygmi and axial lymphadenitis. Laboratory examination: Fecalysis - Protozoal infection Diagnosis: Parvovirosis complicated with protozoan infection Prognosis: Unguarded Recommendations/Remarks: Infusion, metronidazole, Canglob P®. The patient smelled like fresh from bathing when admitted of which it should not have been done. Pets are not to be bathed if ill because this might add up to the stresses. Charting: 15 November. 10:10am – Fluid infusion 10:12am – Medication: 0.6cc Biodyl®, 2.2cc Canglob P®, 0.6cc vitamin K, 5.5cc metronidazole, 0.6cc Ornipural® IV Vitamin K or phytomenadione was given for impaired hemostasis as seen with its history of chocolate-like diarrhea. Ornipural® is a preparation for stressed patients and helps in hepatic function. 06:09pm – Tachypneic breathing 06:10pm – Defecation of brownish liquid feces 06:11pm – Cold limbs; hot pack application 06: 12pm – Recumbency 06:16pm – Expiration (Intern: Crista Iguid) Patient Information: Ruka, a three-month-old white female mixed-bred of Japanese Spitz and terrier Fig. 12. Ruka on the examination table for history and physical examination. Anamnesis: It was inappetent last Saturday; anorexic and weakness progressed the next day; had been voiding light brownish to reddish liquid stools. Multivitamins was administered prior to check-up. Physical Examination: It weighed 2.9kg and had a temperature of 39.2ºC. The patient was lethargic, had tented skin due to dehydration, pale mucous membrane with capillary refill time of more than three seconds, bilateral ocular discharges, clean ears, dry planum nasale and non-auscultation of borborygmi. Laboratory Examination: Fecalysis - Proglottids on freshly voided feces Immunocomb® test- Positive for parvovirosis Tentative Diagnosis: Parasitism Prognosis: Guarded Recommendations/Remarks: Intravenous fluid infusion, metronidazole, Biodyl®, Canglob P®, Ornipural®, vitamin K. The owner claimed that the seller of the dog dewormed it but with the voided proglottids, standard deworming is doubtful. Being loaded with parasites, the dog was predisposed to viral/bacterial/protozoan infection. Charting: 15 November. 04:15pm – Fluid infusion of 5% dextrose lactated Ringer’s solution 04:20pm – Medication: 0.3cc Biodyl®, 1.16cc Canglob P®, 0.3cc vitamin K, 3cc metronidazole and 0.3cc Ornipural® all given intravenously 05:00pm – Voided proglottids in mucoid brown liquid feces 05:15pm – Vomited undigested oatmeal in a pinkish liquid 07:30pm – Provide hot packs 07:35pm – Medicated with 0.6cc metoclopramide IV 08:07pm – Voided mucoid brown liquid feces 10:07pm – Vomited frothy clear liquid 03:08am – Increased respiratory rate of 37bpm 04:31am – Medicated with 0.6cc metronidazole IV 05:16am – Died; heartbeat ceased; no pupillary reflex (Intern: Crista Iguid) #11-017 – Brewdy, four-month-old black Labrador weighing 7.5kg Complaint: Inappetence for three days Anamnesis: Vomiting for two days; cough Diet: Table scraps Temperature: 38.5ºC General Appearance: Alert Integumentary System: Clean Mucous Membrane: Pinkish CRT: less than 3sec Fecalysis: 0-1 ovum Ancylostoma spp. Diagnosis: Parasitism Charting: 08 November. 05:30pm – Intravenous infusion of fluid; drug administration of Biodyl®, Ilium Spasmogenic® and enrofloxacin O9 November. 08:30am – Same line of treatment 09:30am – Urinated (yellowish); defecated (loose) 12:50pm – Forced feeding of molasses 01:00pm – Drank water 04:30pm – Administered Biodyl®, enrofloxacin and metronidazole *transferred to Infectious Ward being suspected of parvovirosis 10 November. 08:00am – Forced fed molasses and Pyramid Hill®; drank water; HR-112BPM; RR-19BPM; temperature-38.6ºC; defecated (bloody) 09:00am – Infused intravenously 5% dextrose lactated Ringer’s solution; administered Biodyl® and enrofloxacin; ate Pyramid Hill®;drank water with molasses 04:00pm – Administered enrofloxacin 05:25pm – Discharge with take-home medicines: enrofloxacin-#3, 1 tab s.i.d Immunol-#14, 1 tab b.i.d. dextrose powder-I bottle (Intern: Venus Casao) #11-050 – Yogi, a two-month-old white male mongrel weighing 1.9kg Fig. 13. Yogi voided adult roundworms. Complaint: Inappetence Diet: Table scraps Fecalysis: Soft light brown; 0-1 ovum Taenia spp Differential Diagnosis: Parvovirosis; parasitism T: 38.9ºC CRT: >3sec Charting: 14 November. 01:00pm – Intravenous infusion of fluid; drug administration of Biodyl® and metronidazole 02:00pm – Administered metoclopramide, vitamin C; dewormed 10:10pm – Defecated roundworms in a foul-smelling diarrhea; administered Canglob P® 15 November. 08:15pm – Provided hot packs; 37.6ºC; HR-150BPM; RR-21BPM 08:50am – Infused fluid intravenously; administered Biodyl® and metronidazole 12:00pm – Vomited (frothy) 01:10pm – Drank water 10:55pm – Fecalysis (rod and cocci bacteria and unidentified protozoa seen under oil emulsion) 16 November. 12:15am – Drank water 07:15am – T-38.8ºC; HR-155BPM; RR-32BPM 07:38am – Forced fed with 1ml molasses 09:30am – Intravenous infusion of fluid; administered Biodyl®, metronidazole and cimitidine 01:48am – Ate table food 02:00am – Vitamin C administration 03:00am – IV infusion stopped; ate rice with meat and vegetable 09:00am – T-38.1ºC; HR-127BPM; RR-32BPM 09:30am – Ate rice with meat 09:45am – Urinated 10:30am – Administered cimetidine; forced fed molasses 17 November. 02:00pm – Administered metronidazole and B-complex vitamins; urinated; ate rice with meat and vegetable; drank water, walked and exposed to the sun; discharged (Intern: Venus T. Casao) #11-060: Pearl, white and black female Shih-Tzu Complaint: Consultation being its littermate diagnosed with parvovirosis Laboratory: Immunocomb® test kit - positive for parvovirosis and canine distemper CBC Hematocrit – 38 PCV – 38 Haemoglobin – 12.6 MCV – 60.31 RBC – 6.3 MCH – 20 WBC – 4 MCHC – 33.15 Charting: 17 November. 05:00pm – Administered Canglob P®, metronidazole and Amitop® 06:30pm – Forced fed by molasses 07:00pm – Drank water; urinated 11:30pm – Provided hot packs 18 November. 12:30pm – Administered Gembifer®; urinated 01:01pm – Drank water with dextrose powder 01:26pm – Ate rice 05:54pm – Administered metronidazole; discharged (Intern: Venus T. Casao) November 7, 2011 Log Number: 06-183 Client: Amelita Icarangal Patient: Shadow; Male; 8 years old; 19 kgs Fig. 14 – 15. The patient at home taking a walk. According to the owner, she noticed that her dog always vomitted right after he finished his food since May 2011. During physical examination the following observations were taken: emaciated but very active, responsive, and apparently normal. X-ray result revealed that the stomach and the upper duodenal part was inflammed and with ulcers based on the interpretation of the radiologost. Fecalysis result revealed that the dog was also positive for hookworm (Ancylostoma caninum 0-1). The dog was dewormed and the resident vet advised to the owner that the treatment program for her dog would be a long-term treatment, so continuous medication plus soft diet was recommended. Take-home medicines were the following: a. Omeprazole (#7) - To be taken 30 minutes before meal once a day for a week. It blocks the final step in acid production, thus reducing gastric acidity. b. Hepasyl (#7) - once a day for a week to protect the liver c. Mondex/Oral Dextrose (1 bottle) - mix in drinking water (2tbs/glass of water) daily to rehydrate the dog d. Molasses (1 bottle) - 5ml once a day to strengthen the animals body condition; act as an appetite stimulant e. Pyramid (1 can) - Soft diet The owner returned after a week, and according to her, there was vomiting after eating but stopped in two 2 days after taking the take-home medicines. After a week the owner returned at the clinic to buy Hepasyl (#7), dewormer (#3), and omeprazole (#7) to continue her animal’s treatment. (Intern: Maria Cristina Beltran) November 8, 2011 Log Number: 11-015 Client: Edgar Pico Patient: Raffy; Male; 9 weeks old; 4.6 kgs The complaint of the owner are the following: inappetence for two days, vomiting (frothy) for a day and foul smell loose stool. During physical examination the following observations were taken: Dehydrated (5%), weak, with external parasites, pale mucous membrane, dry nasal plane, empty stomach, and slow CRT (3sec). Fecalysis result revealed that the puppy was positive for Ancylostoma caninum(0-1). Vitamin supplementation (Biodyl - 0.5ml, IV, SID; Ornipural – 0.5ml, IV, SID) and prophylactic drugs (metronidazole – 7ml, slow IV, BID; metoclopramide - 0.4ml, IV, SID) were given to the dog via the IV line (0.9% saline) for 2 days. On the 2nd day the owner was adviced to take home his dog because he can eat already and also to avoid nosocomial infection because we have four patients that were all highly positive for parvoviral infection. The deworming schedule was scheduled after a week. The following were the take home medicines: a. Metronidazole (#5) - To be taken ½ tab twice a day - An antibiotic, anti-amoeba, and anti-protozoal drug b. Vitamin supplementation (LC-vit) - 1ml once a day; to strengthen the animals body condition. c. Mondex/Oral Dextrose (1 bottle) - To be mix in drinking water (2tbs/glass of water) daily to rehydrate the animal d. Molasses (2ml) - to strengthen the animals body condition; acts as an appetite stimulant. e. Pyramid (1 can) - Soft diet Tentatively the disease of the puppy can be a mild respiratory infection and/ viral infection due to the early signs observed by the owner and during hospitalization. (Intern: Maria Cristina Beltran) November 9, 2011 Log Number: 11-022 Client: Carlos Fernando Patient: Budoy; Male; 2 weeks old; 4.4 kgs Fig. 16 – 18. Recovering Budoy voided semi-solid feces. The complaint of the owner were the following: inappetence for a week, vomiting (frothy) for 2 days and foul smell loose stool. During physical examination the following observations were taken: dehydrated (8%), weak, with external parasites, dry and dirty coat, pale mucous membrane, dry nasal plane, empty stomach, and slow CRT (3sec). It was also noticed that the upper jaw was inflammed, the permanent toothe starts to come out. Fecalysis result revealed that the puppy was positive for Ancylostoma caninum(0-1) and Toxocara canis (0-1). Vitamin supplementation (Biodyl - 0.5ml, IV, SID; Vit. K – 0.5ml, IV, SID; Ornipural – 0.5ml, IV, SID) and prophylactic drugs (metronidazole – 6.6ml, slow IV, BID; Ilium Spasmolytic – 0.5ml, IV, SID; Canglob P – 1.8ml, IV, SID) are given to the dog via the IV line (D5LRS) during the first 2 days. On the 3rd day only the metronidazole, Biodyl, Canglob P, and Ornipural were given in the morning. The puppy starts to eat little so addition of molasses (2ml, PO, BID) to the diet was incorporated; Rimadyl (1tab,SID) was also given to stop inflammation on the upper jaw. On the 4th-6th days of confinement, all drugs (B-com – 1tab, SID; Liv 52 – 1tab, SID; Rimadyl – 1tab, SID, Metronidazole – ½ tab, BID) are given via oral route. On the 6th day, enema was applied because he cannot defecate, then after 5 minutes he defecated already. Budoy was discharged on the 6th day and dewormed. There were take-home medicines for Budoy, but the owner said that he cannot afford to pay all the bills plus the medicines. Tentatively, the disease of the puppy can be early stage of viral infection (parvoviral infection) because the dog showed a response to the drugs given to him after 48 hours of hospitalization and oral infection due to the errupting permanent tooth. (Intern: Maria Cristina Beltran) November 9, 2011 Log Number: 11-034 Client: Derek Amango Patient: Helsing; Male; 7 months old; 17 kgs Fig. 19 – 20. Recovering Helsing. The complaint of the owner were the following: inappetence for 2 days, vomiting (watery) for a day. Not yet dewormed and without any vaccination. During physical examination the following observations were taken: alert/apparently normal, pale mucous membrane, dry nasal plane, empty stomach, wheezing sound during respiration was heard during auscultation, and slow CRT (3sec), the dog also had a fever. Fecalysis result revealed that the puppy was positive for Ancylostoma caninum(0-1) under HPO. Days 1 and 2, I gave paracetamol (1.7ml, IM,SID); Biodyl (1.7ml, IV, SID); and Marbocyl (1.7ml, IM, SID). On the 3rd-4th day the dog can already eat, so all treatments were given via the oral route. It was discharged on the 5th day. The following were the take home medicines: a. Molasses (1 bottle) - 5ml once a day to strengthen the animals body condition; act as an appetite stimulant. b. Carnitab (#7) - Once a day c. Pyramid (1 can) - Soft diet The owner returned the dog after a week for deworming. Tentatively, the disease of the dog was respiratory infection based on the observations and type of respiration exhibited by the dog and also parasitism. (Intern: Maria Cristina Beltran) November 15, 2011 Log Number: 11-054 Client: Joselito Castillo Patient: Tiny; Female; 3 months old; 1.4 kgs Fig. 21 – 22. Tiny before admitted and when admitted. Fig. 23 – 25. The test kit used for the confirmative diagnosis for parvovirosis. The complaint of the owner were the following: inappetence for 4 days, vomiting (frothy) for 2 days, and foul smell loose stool. It was not yet dewormed and vaccinated. During physical examination the following observations were taken: weak, shivering, pale mucous membrane, dry nasal plane, empty stomach, and slow CRT (3sec). Fecalysis result revealed that the puppy was positive for Toxocara canis (0-1) under HPO. The puppy was also highly positive for parvoviral infection. Vitamin supplementation (Biodyl - 0.15ml, IV, SID; Vit. K – 0.35ml, IV, SID; Ornipural – 0.15ml, IV, SID) and prophylactic drugs (metronidazole – 2.1ml, slow IV, BID; Canglob P – 1.8ml, IV, SID) were given to the puppy via the IV line (0.9% saline) during day 1. On day 2, the puppy started to eat but little so I gave 1ml of Molasses (BID) to stimulate the appetite of the animal. Drugs given were LC-vit (1ml, SID) and metronidazole (0.18ml, BID). The puppy was ready for discharge on the 3rd day. The following were the take home medicines: a. Molasses (1 bottle) - 1ml once a day to strengthen the animal’s body condition; acts as an appetite stimulant. b. Flagex/Metronidazole (1 bottle) - 0.18ml, BID; an anti-biotic, anti-amoeba, and anti-protozoal drug. c. Pyramid (1 can) - Soft diet d. Vitamin supplementation (LC-vit) - 1ml, SID; to continuously strenghen the animal’s body condition. The owner was adviced to returned his puppy after a week for deworming and for immunization. The disease of the dog was parvoviral infection as revealed by the kit used. (Intern: Maria Cristina Beltran) November 16, 2011 Log Number: 11-060 Client: Armando Punzalan Patient: Hillary; Female; 7 weeks old old; 1.7 kgs Fig. 26 – 28. The admitted patient being infused with intravenous fluid and its semi-solid feces. The complaint of the owner were the following: inappetence for two days, vomiting (watery) for two days, and reddish brown loose stool. Not yet dewormed and without any vaccination. According to the daughter of the owner, she gaved about 50 grams of Cadbury chocolate three days before they brought the puppy at the animal hospital. During physical examination the following observations were taken: weak, shivering, pale mucous membrane, salivation, dry nasal plane, empty stomach, and slow CRT (3sec). Fecalysis result revealed that the puppy was positive for Ancylostoma caninum (0-2) under HPO. The puppy was also highly positive for parvoviral infection. Vitamin supplementation (Biodyl - 0.17ml, IV, SID) and prophylactic drugs (metronidazole – 2.6ml, slow IV, BID; Canglob P – 0.7ml, IV, SID; metoclopramide 0.14ml, IV, as needed) were given to the puppy via the IV line (0.9% saline) and atropine (0.2ml, SC, once) during day 1. On the 2nd day, the puppy still didn’t like to eat and still vomiting (frothy) so same medications were given to her except the atropine. On the 3rd day, I gave her anti-viral drug (Canglob P) 0.7ml via the SC route and she can eat already so oral medications were given to her (Flagex/metronidazole - 0.18ml, BID; Gembifer - 0.5ml, SID). The puppy was ready for discharge on the 4th day. The following are the take home medicines: a. Flagex/metronidazole (1 bottle) - 0.18ml, BID; an anti-biotic, anti-amoeba, and anti-protozoal drug. b. Mondex/oral dextrose (1 bottle) - To be mix in drinking water (2tbs/glass of water) daily to rehydrate the animal c. Gembifer (1 bottle) - 0.5ml, SID; to continuously strenghen the animal’s body condition. e. Pyramid (1 can) - Soft diet The owner was adviced to returned his puppy after a week for deworming and follow-up check up. The disease of the dog was parvoviral infection as revealed by the kit used. (Intern: Maria Cristina Beltran) November 18, 2011 Log Number: 11-073 Client: Julius Caliguing Patient: Chloe; Female; 1 year and 1 month old; 3.8 kgs Fig. 29 – 34. The patient showing the injured eye. According to the owner, his dog’s eye was scratched by the other dog 3 days before he brought Chloe at the animal hospital. During physical examination on the eye, it was observed that the affected eye still responsed/moved to the light (penlight) used to checked the vision. So Dr. J. Runas instructed me to just clean the affected eye with clean cloth soaked in luke warm water to removed the dirt and to cut the hairs surrounding the affected eye to avoid contamination. Before cleaning the eye, we injected Tolfine (0.5ml, IM) and Amoxicillin LA (0.5ml, IM). After cleaning the eye, we put 1 drop of eye dropper (Timolol). Take home medicines are the following: a. Vitamin supplementation (LC-vit) - 1ml, SID; to continuously strenghen the animal’s body condition. b. Rimadyl - 1 tab daily for 5 days; an anti-inflammatory drug. c. Amoxicillin capsule (250mg) - 1 cap daily for 5 days; an anti-biotic. d. Timolol - 1 drop twice a day after cleaning the eye with cloth soaked in luke warm water. The owner was adviced to returned his dog 1 week after for follow-up check up. (Intern: Maria Cristina Beltran) November 19, 2011 Log Number: 11-078; OPD Client: Aprylle Kate Munoz Patient: Hatchiko; Male; 5 months old; 4.9 kgs Fig. 35 – 40. Showing the yellowing gum, conjunctiva, and paw. The complaint of the owner are the following: inappetence for 5 days and reddish brown loose stool. Not yet dewormed and without any vaccination. They just got the puppy on the garbage 2 months ago. During physical examination, it was observed the yellowish discoloration of the gums, paws, inguinal part and the eye. When Dr. Melly saw the puppy and the PCV, she told the owner that her dog will be subjected to euthanasia because the dog might have Canine Infectious Hepatitis based on the color of the puppy during physical examination and it can be transferable to human. The owner was also advice to disinfect their house and the sorroundings of their house. (Intern: Maria Cristina Beltran) B. Non-infectious Patient: Yankee Procedure/s done: Its weight and temperature were taken prior to vaccination and deworming. (Intern: Crista Iguid) Patient: Curvy Procedure/s done: Its weight and temperature were taken prior to vaccination and deworming. (Intern: Crista Iguid) Patient: Boycha Procedure/s done: Its weight and temperature were taken prior to vaccination and deworming. (Intern: Crista Iguid) Patient: Nike, a one-year-old Bassethound Anamnesis: Being a first timer bitch, Nike had a hard-time whelping. It whelped its first pup independently but the gap for the birth of its second pup alarmed its owner bringing it to the animal hospital for assistance. Procedure/s done: Oxcytocin was administered to help Nike induce loosening of its pelvis and dilatation of cervix. Whelping was over at around 4:30pm. Eight pups were delivered. Its last pup seemed mummified. (Intern: Crista Iguid) Patient: Twinkle, a seven-month-old tricolor female mongrel Anmanesis: It was admitted for the chief complaint of having been eaten a plastic and became anorexic. Fecal sample was brownish red. Procedure/s done: The line of treatment was as follows: Metronidazole – 10ml, slow IVb.i.d. Septotryl® – 1ml, slow IV Vitamin K – 0.1ml, IV Biodyl® – 1ml, IV A wound was seen on its medial right hindlimb. It was dabbed with iodine. It was discharged after its appetite got okay. (Intern: Crista Iguid) Patient: Sharky, a three-month-old tuxedo male mongrel Procedure/s done: Its vaccination was updated. 6-in-1/CPV-DHLP-CV (Canine ParvovirusDistemper-Hepatitis-Leptospirosis-Parainfluenza-Coronavirus) and antirabies shots were given. (Intern: Crista Iguid) Patient: Holie, one-year-seven-month-old mongrel Procedure/s done: Antirabies was given. (Intern: Crista Iguid) Patient: Justin, a six-month-old brown male mongrel weighing 6kg Fig. 41 – 42. Justin admitted for the first day before the abdominocentesis (right photo) and the aspirated fluid (left). Anmanesis: It was admitted for its distended abdomen. Procedure/s done: Charting: 09 November. It was confined in the non-infectious area, infused with 5% dextrose lactated Ringer’s solution and medicines were as follows: Furosemide – 2cc, IV Ornipural® – 0.6cc, IV Biodyl® – 0.6cc, IV s.i.d. 10 November. 500ml was aspirated intra-abdominally. Urine was continuous dripping from its penis and also from the puncture area. It was walked outside for a while and was tried to be fed with mashed Pyramid Hill®. Since it had a good appetite, Sambong® was given and also dewormed, Liv-52®, good for the liver, was also given PO. It was fed with a little of Pyramid Hill® at around 04:00pm. 11 November. It was fed with mashed Pyramid Hill®. It was medicated with Sambong® and Liv-52®. 12 November. It was brought outside for sun bathing and exercise. While it was tied, I tidied up its cage. I noticed numerous drippings of urine that dried up and the poop was seemed to be hard. Justin was fed with Pyramid Hill® mashed in lukewarm water. It was medicated with each tablet of Sambong® and Liv-52®. Sambong®, Blumea balsamifera, is categorized as diuretic or anti-urolithiasis. It is indicated for urinary tract pain and burning sensations, to increase urinary output in condition characterized by fluid retention or edema and for kidney stones. Liv-52® contains herbs indicated for hepatic damage and dysfunction, ascites, jaundice and cirrhosis of liver: adjuvant in the management of canine and feline viral hepatitis, distemper and parvovirus infection; anorexia or hepatic origin, convalescence following debilitation; supportive therapy in antibiotic and antiparasitic drug therapy, hematoprotective in toxicity associated with drugs, pesticides and other chemicals; as growth promoter and metabolic stimulant and as supportive therapy in post-operative care. At 1:00pm, it defecated. Feces was loose and unformed. Catheter was removed after no resistance was sensed upon insertion. Abdominocentesis was done after whereon same color of fluid of about 20ml was aspirated from almost same site of puncture as of November 09. DL-methionine® was opted over Sambong®. DLmethionine® is a urinary acidifier that assists in the treatment of cystitis and alkaline susceptible urinary calculi in dogs and cats. It also aids in the prevention of fatty liver in dogs. For the afternoon, medications included Rimadyl® which is a carprofen. 13 November. Justin was fed with same diet. After about thirty minutes after eating, it vomited what it ate then defecated. Water was provided. DL-methionine® was given since Sambong®, B-complex and Liv-52® were vomited. Molasses was also given per orem. 14 November. It was fed and medicated then brought outside. Feces seen were unformed so metronidazole was added to the usual medicines. The owner was notified for its possible release for the day so bills were prepared and so with the take-home medicines comprising of Sambong® good for 7 days, 1 bottle of molasses, B-com good for 5 days for continued neural repair. It was weighed as 5.1kg that had decreased from 6.6kg from its first day. (Intern: Crista Iguid) Patient: Whity, a white female mongrel pup Procedure/s done: Deticking, de-flea-ing and deworming (Intern: Crista Iguid) Patient: Saki, a five-month-old brownish mixed-bred dog Procedure/s done: It was castrated. (Intern: Crista Iguid) Patient: Shamcey Procedure/s done: It was vaccinated with 5-in-1/CPV-DHLP (Canine ParvovirusDistemper-Hepatitis-Leptospirosis-Parainfluenza). (Intern: Crista Iguid) #11-038 – Zannah, a six-month-old female tri-color terrier weighing 15.2kg Complaint: Pus dripping from genitalia Anamnesis: Pus was seen the day before; inappetence then set in Diet: Table scraps Last Vaccination: Antirabies (Rabisin®) Diagnosis: Pyometra Charting: 11 November. Vaginal lavage; administered B-complex vitamins 12 November. Uterine lavage; administered B-complex vitamins, vitamin C, Uterex® and amoxicillin LA 19 November. Follow-up check-up; uterine lavage; administered B-complex vitamins, vitamin C and Uterex®; take-home medicines of #4 amoxicillin LA ( 1 tablet b.i.d.), #7 Pet-tabs® ( 1 tablet s.i.d.) and #4 mefenamic acid ( 1 tablet b.i.d.) (Intern: Venus Casao) #6041: Aki, brown Chow-chow Laboratory: negative for fecalysis (Intern: Venus Casao) November 7, 2011 1. Log Number: 11-012 Client: Jonathan Villagracia Patient: Kobi; Male; 1 ½ months old - Deworming (1/2 tab Proxantel); Vaccination (5 in 1) 2. Log Number: Client: Benito Beniking - 3 sacks of Strike 3. Log Number: Client: June Sab-it - Semen; AI catheter (Intern: Maria Cristina Beltran) November 8, 2011 1. Log Number: 11-016 Client: Florian Leygo Patient: Chacha; Female; 7 weeks old - Vaccination (5 in 1) 2. Log Number: 10-174 Client: Jane Ferrer Patient: Bambi; Male; 2 months old - Deworming (1/2 tab Proxantel); Bought Bronchure (Intern: Maria Cristina Beltran) November 9, 2011 1. Log Number: 11-020 Client: Jhun-jhun Cosme Patient: Kobi; Male; 2 ½ months old - Deworming (Ivermectin); Vaccination (B-com supplementation) 2. Log Number: 11-021 Client: Junella Meman Patient: Lily; Female; 2 years old - Vaginal Smear (Pro-estrus); Bought Vitamin(LC-vit) 3. Log Number: 11-025 Client: Ronnie Bassit Patient: Holie; Female; 3 years and 8 months old - Deworming (3 ½ tab Proxantel); Vaccination (Anti-rabies) Log Number: 11-031 Patient: Lust; Male; 5 years old - Deworming (4tab Proxantel); Vaccination (Anti-rabies) Log Number: 11-032 Patient: Lassy; Male; 6 years old - Deworming (4½ tab Proxantel); Vaccination (Anti-rabies) (Intern: Maria Cristina Beltran) 4. Log Number: 11-033 Client: Montgomery Mang-osan Patient: Bruno; Male; 2 months old - Ear suturing (Left ear has been torn/bitten by the mother of the puppy) (Intern: Maria Cristina Beltran) 5. Log Number: Client: John Masiong - Semen; AI Catheter (Intern: Maria Cristina Beltran) November 11, 2011 1. Log Number: 11-040 Client: Elizabeth Salasa - Bought Ivermectin (.5ml) and 3ml syringe 2. Log Number: 10-028 Client: Ann Btinay Patient: Shamcey; Female; 3 months old - Vaccination (5 in 1; Anti-rabies) 3. Log Number: Client: Evelyn Ngilangil Patient: Pups; Female; 4 months old - Vaccination (Bronchicine) (Intern: Maria Cristina Beltran) November 14, 2011 1. Log Number: 11-052 Client: Julia Agayo Patient: Whity; Female; 5 months old - Deworming (1/2 tab Proxantel); Bought vitamin (LC-vit) 2. Log Number: 11-007 Client: Junella Meman Patient: 2 puppies; Female and Male; 2 weeks old - Tail docking (Intern: Maria Cristina Beltran) November 15, 2011 1. Log Number: 11-053 Client: Joann Tip-ac Patient: Trisha; Female; 3 years old - X-ray (Referral – Pregnancy diagnosis) (Intern: Maria Cristina Beltran) November 17, 2011 1. Log Number: 11-065, 066, 067, 068, 069 Client: Dominga Cachero Patient: Puppy 1, 2, 3, 4, 5; Male, Male, Female, Female, Female; 6 wks. old all - Deworming (1.3ml Wormban each); Vaccination (5 in 1) Log Number: 04-146 Patient: Kidor - Vaccination (Bronchicine) (Intern: Maria Cristina Beltran) November 18, 2011 1. Log Number: 11-077 Client: Vianney Basikinas Patient: Puppy; Male; 2 months old - Deworming (1/2 Tab Proxantel); Bought vitamin (LC-vit) (Intern: Maria Cristina Beltran) November 19, 2011 1. Log Number: 11-078 Client: Apryl Kate Munoz Patient: Hachiko; Male; 5 months old - For check-up (Positive for infectious canine hepatitis) - Eutahnasia 2. Log Number: 11-079 Client: Roanne Rubang Patient: Hatchy; Male; 2 months old Deworming (1/2 tab Canex); Bought vitamin (Liv 52 – 7 tabs; LC-vit) and Pyramid Hill 3. Log Number: 10-079 Client: Montgomery Mang-osan Patient: Bubbles; Female; 3 weeks old - For check-up (ehrlichia kit); Negative result 4. Log Number: 11-080 Client: Sheila Rose Basilio Patient: Russele; Male; 8 weeks old - Vaccination (6 in 1); Bought vitamin (LC-vit) 5. Log Number: 11-081 Client: Janelle Marzan Patient: Troy; Male; 7 weeks old - Vaccination (5 in 1); Bought vitamin (LC-vit) (Intern: Maria Cristina Beltran) - Aquatic Station: Bureau of Fisheries and Aquatic Resources Regional Mariculture Technodemo Center Lucap, Alaminos City, Pangasinan and Fish Health Laboratory Dagupan City, Pangasinan On 21 November-03 December 2011 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE ESTABLISHMENT The BFAR is the government agency responsible for the development, management and conservation of the country’s fisheries and aquatic resources. It was reconstituted as a line bureau by virtue of Republic Act No. 8550 (Philippine Fisheries Code of 1998). The bureau is under the Department of Agriculture. The entire Philippines was divided into ten fishery districts, each with a District Fishery Officer as head. The headquarters of the ten fishery districts were located in strategic places in the different fishinf regions: Fishery District No. 1-Aparri, Cagayan; 2-Dagupan City; 3-Manila; 4-Naga City; 5-Catbalogan, Samar; 6-Iloilo City; 7-Coron, Palawan; 8-Cebu City; and 10Zamboanga City. The facilities, located at the Regional Mariculture Technology Demonstration Center (RMaTDeC), include a land-based mariculture holding facility, plastic liner covered pond, abalone hatchery, siganid hatchery, aqua zoo, and an administration training and dormitory building. II. A. DUTY STATIONS Food Preparation Activities: Culture of Rotifer species. These are food for the Chlorella species. Culture of Chlorella species. These are food for the juvenile aquatic species. B. Production Abalone: Cleaning of ponds. After the juvenile abalones are fished out from the tanks, the tanks are drained for refilling of clean water. Feeding the breeders with seaweeds. After the tanks are refilled with water, seaweeds good for 24-hour consumption are provided. Checking for gravid abalone for breeding. Gravid abalones are contained in a separate net for breeding. Sizing and counting of juvenile abalones. Juvenile abalones are fished out from the tanks and are sorted. Tilapia: Feeding. Since tilapia are surface feeders, floating feeds are provided. Sea Cucumber: Spawning. Batches of 30-45 individuals (average weight 500g) are needed to induce spawning. When relying on wild broodstock, ripe and healthy broodstock must be collected during the spawning season. Transport wild broodstock individually in oxygen-filled bags with seawater, using insulated containers at 27-30ºC. Avoid changes in temperature and other shocks during transport. Broodstock can be conditioned by keeping them in: (a) tanks (15-30animals/1000l) with a sand or mud substratum, with flow-through seawater and a supply of food; (b) sea pens of 800m2 at densities of <200g/ m2; and (c) earthen ponds at densities of <250g/m2 with continuous water exchange. Ponds used to hold broodstock require management to avoid high temperatures and low dissolved oxygen caused by stratification due to heavy rain. Salinity must be maintained within the range of 28-36ppt. Seabass: Feeding. Sinking feeds are provided for these fishes. Sizing. The juvenile fishes are fished out from the pond to be sorted. Bigger ones are released into the tanks labelled for such size. Smaller ones are released back into the pond. C. Water Quality Monitoring. Shellfish collection and labelling of samples for toxin analysis. A cluster of green mussels (Perna viridis) set in a basket should be hanged at the intermediate depth from a bamboo pole or a raft. The depth of the settlement of the net is depending on the total depth of each station established in the sampling areas. The green mussels in the basket nets will be regularly samples for paralytic shellfish poisoning (PSP) toxin monitoring. If the green mussels are not available, oysters are also used for PSP toxin monitoring the same in green mussels. Methods of Collection: 1. Collect about 20-30specimens of green mussels (the number depends on the size, as long as it will give a tissue of about 100g) weekly from pre-established sampling stations in affected waters. 2. Clean the fresh shellfish by scrubbing the mud, silt and other adhering materials. 3. Keep the shellfish samples in a net bag or plastic container with proper label and store in a cooler while on board. Plankton sampling. Plankton samples can be collected by hauling the plankton net (20um mesh size) vertically at least 1.5m above the sea bottom to the surface water to qualitatively observe/determine the population of toxic dinoflagellate species. By the same technique, a large amount of cells of the toxic species can be collected by repeating the vertical hauling for toxin quantification to confirm the toxicity of natural cells. In order to compare the toxicity in planktonic cells with that in the shellfish, it is necessary that this collection must be done exactly where the shellfish samples are collected. Operation of plankton net: 1. Determine the depth of the sampling station. 2. Tie the plankton net with a calibrated rope (1-meter interval) and tie a 1kg weight to the narrow end of the net to make it sink in the water. 3. Lower the plankton net to the desired depth with at least 1m clearance from the bucket and sea bottom. Once the total sampling depth is known, a 1m depth should be subtracted from the sea bottom. This 1m is the total of the clearance and length of the plankton net. For instance, given a total depth, which means an allowance of only 1 m from the sea bottom should be made. The length from mouth of the net to the cod end is 0.5m, therefore plankton is collected 1.5m above the bottom of the ocean to the surface of water. 4. Haul the net slowly (0.5m/sec) and vertically up the surface. 5. Raise and wash the net and its cod-end several times by shaking the net in the water. 6. Transfer the concentrated samples in a 500ml polyethylene bottle and keep under cool condition. Water Depth. Measurement of water depth is also important to know the effect of tidal change of each station and to determine the vertical depth of plankton net hauling. In order to measure the depth of the established stations, lower a weight of 5001000g that is tied at the one end of a calibrated rope. The depth can be obtained at the level where the sounding line stops. Transparency. A Secchi disk is slowly lowered at the sheltered side of the boat, and the exact depth where the disk is no longer visible is recorded. Determine the water transparency by getting the length of which the white disk is last visible from above. Water temperature. Using the alcohol type thermometer, determine the temperature of surface water. Salinity. Data on salinity provides information on the effect of fresh water run-off to the monitoring area. But this important data is optional for economic reason because this entails the use of expensive chemical agents and apparatus during titration analysis. Fig. 43 – 46. Water quality monitoring and sizing of fingerlings. Fig. 47. A sea cucumber ejaculating. Wildlife Station: Department of Environment and Natural Resources Protected Areas and Wildlife Bureau Wildlife Rescue Center and Mini-zoo Quezon Avenue, Diliman, Quezon City On 05 - 17 December 2011 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE FARM The PAWB-WRCM is established as a temporary shelter for donated, rescued, abandoned and confiscated endemic, indigenous and exotic wildlife from Metro Manila and nearby provinces. All wild animals brought into the Center undergo the necessary health quarantine and rehabilitation period. Endemic and indigenous wild animals found physically are eventually released back into their natural habitats following standard protocol. Those unfit individuals as well as exotic ones are displayed in the Mini-zoo to promote public awareness, appreciation and support to the conservation of the country’s wildlife resources and their habitats. The Center is also a source of display animals for legitimate zoological parks and research specimens for academic and scientific institutions. It is also a living laboratory for veterinary and biology students and other wildlife enthusiasts. *Endemic wildlife – species or subspecies which is naturally occurring and found only within specific areas in the country *Indigenous wildlife – species or subspecies of wildlife naturally established population in the country *Exotic wildlife – species or subspecies which do not occur naturally in the country II. PAWB-WRCM DUTY STATIONS A. Clinic and Quarantined Animals Activities: Cleaning the aquariums and changing the water for the turtles Dispose excreta from under the animals’ cages Sweep dried leaves around the premises of station Provide food and water for the caged birds Wrapping with proper labelling of expired patients Medication of patients Accepting and properly recording of donated, rescued or animals B. Primates and Snakes Activities: Cleaning the cages/terrariums Provision of drinking water Feeding Dispose excreta from under the primates’ cages Sweep dried leaves around the premises of station C. Lizards and Other Mammals Activities: Cleaning the cages Provision of drinking water confiscated Feeding Dispose excreta from under the animals’ cages Sweep dried leaves around the premises of station D. Raptors Activities: Flushing off of excreta on the cages Provision of drinking water Feeding Sweep dried leaves around the premises of station E. Avian and Turtles Activities: Changing the aquarium’s water; flushing off of the avian excreta Feeding Sweep dried leaves around the premises of station F. Food Preparation Activities: Washing and peeling fruits and crops readied for chopping Preparing the food supplements for animals that need them most Thawing the frozen goods such as the beef or other meat stored in for the carnivorous animals Slicing the meat into equal proportion G. Necropsy Activities: Performing the necropsies on expired animals with proper documentation Recording of documentation Fig. 48. Feeding the Brahminy kites. Fig. 50. Dressing a wound of a raptor. Fig. 49. Doing the weekly necropsy. Fig. 51. Staining a blood smear. Laboratory Station: Regional Animal Disease Diagnostic LaboratoryCordillera Administrative Region, Santo Tomas Road, Green Valley, Baguio City On 09 - 13 January 2012 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE ESTABLISHMENT Mission: To improve farmers’ income by increasing animal productivity thru provision of efficient and affordable disease diagnostic services Objectives: General: To provide laboratory diagnostic services that supports the research, development and regulatory functions of the Department of Agriculture. Specific: 1. Perform and conduct various laboratory tests which are very necessary in the diagnosis of animal diseases. 2. Conduct research study on animal health and animal diseases. 3. Perform collaborative functions with the Bureau of Animal Industry and other government institutions on animal disease control programs. 4. Serve as training ground for veterinary and agriculture students. II. INTERN ACTIVITIES A. Necropsy B. Rabies Examination 1. Direct Microscopic examination 2. Fluorescent antibody technique C. Veterinary Clinical Parasitology *Microscopic Examination of Feces Simple/direct smear Sugar floatation technique Sedimentation technique Fig. 52 – 53. The making of a sugar solution for the fecal examination. The group staining his/her sample of a tissue swab. Fig. 54. Geared up for the preparation of the sample. Large Animal Practice Ruminant Station: Philippine Carabao Center-CLSU National Bull Farm, Digdig, Carranglan Nueva Ecija On 29 January – 18 February 2012 Group Members: Beltran, Maria Cristina C. Casao, Venus O. Iguid, Crista M. Panawan, Clarence N. I. DESCRIPTION OF THE ESTABLISHMENT In 1992, after PCARRD-coordinated UNDP-FAO project, the Philippine government realized the need to institutionalize the Carabao Development Program. Through Republic Act 7307 or better known as the Philippine Carabao Act of 1992, the Philippine Carabao Center was born. The law was signed on March 27, 1992 and operationalized on April 1, 1993. Mandate: The Philippine Carabao Center is an attached agency of the Department of Agriculture. PCC is mandated to conserve, propagate and promote the carabao as a source of milk, meat, draft and hide to benefit the rural farmers. Vision: A premier institution promoting profitable and sustainable carabao-based enterprises designed to improve the income and nutrition of rural farming communities. Mission: To improve the general well-being of rural farming communities through carabao genetic improvement, technology development and dissemination and establishment of carabao-based enterprises thus ensuring higher income and better nutrition. II. INTERN ACTIVITIES A. Animal Health Section Activities: Vaccination. Vaccination for the year was completed so the group was not able to administer shots for the carabao. Medication. With two groups in 2s in a rotation manner, Clarence-Crista group was able to help in the medication of three cara-bulls suspected of schistosomiasis. At a dose of 40mg/kg, the computed doses in tablets of praziquantel were powdered and mixed in feeds to mask the drug. Another cara-bull with limping on the right hindlimb on the hock was medicated with dexamethasone and norfloxacin. 40ml each of the drugs were given intramuscularly for two days b.i.d. Blood collection. On February 12, all the cara-bulls were subjected for blood sampling. Together with two sixth year student clinicians from Central Luzon State University, Clarence-Crista group assisted in the process. Samples were divided into two, blood samples for heparinised testtubes for whole blood sampling and the ones for plain testtubes for serum sampling. Bull preparation prior to semen collection. As part of the artificial insemination bull preparation is very important. A power spray is used to bathe the bulls before collection. Lugol’s solution added to warm water is used to wash the prepuce of the cara-bulls to be collected from through the prepuce washer. Barn cleaning. While the cara-bulls are brought out for sun basking, the barns are cleaned. The excreta are disposed with the use of spade and wheelbarrow. The floors and walls are flushed with water. Feeding and Watering. After cleaning, the waterers are filled with water for ready access of water. First things first, feeds are given in the morning. After which, hays are given dressed with molasses believed to make the hay more palatable. Napier grasses are given later. Later in the afternoon, hays are given again. B. Semen Processing Laboratory Activities: Semen Collection. Semen is collected by the use of artificial vagina twice a week at 6:00 to 7:00 in the morning. Complete bath are given to the water buffalos before actual collection. The parts of an artificial vagina (casing, inner lining, rubber funnel and collecting tube) are first sterilized under ultra violet rays before use. The AV is prepared by filling the interior cavity of inner liner rubber with warm water (4052ºC) and is pumped with an optimum pressure of air. Semen Evaluation. The samples’ macroscopic volume, color, consistency, pH, motility and sperm concentration are recorded. Dilution. Extender and buffer are added to the semen. Extender consists of 20% egg yolk, distilled water, 7% glycerol as cryoprotectant, streptomycin and penicillin. Buffer solution consists of trisodium hydroxymethyl aminomethane, citric acid monohydrate, fructose and raffinose. Filling and Sealing. Straw is filled with semen using vacuum pump. Polyvinyl chloride powder is used for sealing. Color coding is used, yellow for the first ejaculate, red for the second, green for the third and black for the native cara-bull’s. The straws then are placed in the water bath to complete the sealing. The concentration of semen per straw is approximately 50 million. Glycerolization. It starts after the addition of extender with glycerol for at least four hours wherein the glycerol and other components of extender to equilibrate with the spermatozoa. Post Thaw Motility Evaluation. Samples are taken from the sealed straws of semen for evaluation before storage. Storage/Distribution. Straws of frozen semen that passed the evaluation are stored for future use. *A new machine automated for printing, filling and sealing of the straws was just delivered on February 14 and is now on use. So with the new equipment for sterilizing and heating of artificial vaginas is now on use. The new equipments were donated by Korea International Assistance Agency (KOICA). Fig. 55. Bathing a bull from a power spray. Fig. 56. A preputial wash with a Lugol’s solution done everyday. Fig. 57. Performing a rectal examination on a non-pregnant cara-cow. Fig. 58. Administering the norfloxacin and dexamethasone.