CharFac/UIC workshop
Specimen Preparation for Biological TEM and Correlative Light and
Electron Microscopy (CLEM)
Time: 8:30 AM – 4:00 PM (Tentative), Thursday, April 23, 2015
Places: Morning lectures: Mayo 3-100
Afternoon practical session: NHH and Moos Tower
Registration Deadline: April 15, 2015
Description:
The workshop focuses on recent advances for TEM sample preparation and for correlating what
we image in the light microscope with the very same specimen in the electron microscope. We
will show how to prepare cells and tissue samples by plunge or high pressure freezing, fix and
dehydrate them at low temperature, then have them in polymerized resin using freezesubstitution using an automatic device, or rapid methods that can take place in one working day.
These procedures are also well-suited for preserving fluorescence in resins for correlative
studies and on-section immunolabeling. CLEM also can be useful to the research of synthetic
materials such as complex polymeric/organic systems that contain a hierarchy of length scales
relevant to a material’s processing, properties and/or performance, or materials that utilize
fluorescent tagging for biomedical applications. During the workshop we will demonstrate the
sample preparation modalities at UIC and CharFac. We will also highlight the FEI iCorr, a
fluorescence microscope integrated with the Tecnai Spirit TEM at CharFac in its Moos Tower
location. Participants should leave the workshop with practical knowledge of the latest
procedures and equipment for CLEM.
Speakers:
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Kent McDonald, Director of Electron Microscope Lab, University of California, Berkeley.
Talk title: Mythbusters: the electron microscopy episode
Matthia Winter-Karrenman, Post-doctoral researcher, EMBL Heidelberg, Germany
Talk title: Lights will guide you: aample preparation and applications of integrated
correlative fluorescence and electron microscopy
Mark Sanders, Director of UIC, University of Minnesota
Talk title: Correlative imaging through multiple dimensions at the UIC
Gregor Heiss, Product Marketing Engineer for Correlative Microscopy, FEI company
Talk title: CLEM: from living cells to 3D electron microscopy
Levi Felts, Leica Microsystems
Talk title: TBD
Fee:
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Morning lectures are free.
Paid attendees participate hands-on/demo in the afternoon. Limited to 20 participants.
o Academic: $100 (UMN students, post-doc, staff and professor)
$200 (non-UMN researchers)
o Industry: $250 (IPrime members discount rate)
$300 (Non-IPrime members)
Registration:
https://umn.qualtrics.com/SE/?SID=SV_3Wtq0bQ3FLgrrMN
Contacts:
About workshop: Wei Zhang (zhangwei@umn.edu, 612-624-1522)
About registration/payment: Joel Overlander (jgo@umn.edu, 612-625-0776)
Sponsors:
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FEI company
Mager Scientific, Inc.
IPrime's Nanostructural Materials and Processes Program, Leader Alon McCormick
(College of Science and Engineering)
Characterization Facility, Director Greg Haugstad (College of Science and Engineering)
University Imaging Centers, Director Mark Sanders (College of Biological Sciences and
Academic Health Center)