Optimizing cultivation strategies for robust algal
growth and consequent removal of inorganic
nutrients in pretreated livestock effluent
Hyun-Chul Kima, Wook Jin Choib, c, Jun Hee Ryub, Sung Kyu Maengd, Han Soo Kime,
Byung-Chan Leef, and Kyung Guen Songb,*
a
Water Resources Research Institute, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-
747, South Korea
b
Center for Water Resources Cycle Research, Korea Institute of Science and Technology,
Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, South Korea
c
Department of Civil and Environmental Engineering, Yonsei University, 262 Seongsanno,
Seodaemun-gu, Seoul 120-749, South Korea
d
Department of Civil and Environmental Engineering, Sejong University, 98 Gunja-dong, Gwangjin-gu,
Seoul 143-747, South Korea
e
ENR Solution Co. Ltd., 60-15 Gasan-dong, Keumcheon-gu, Seoul 153-801, South Korea
f
Department of Civil Engineering, Suncheon First College, San 9-3 Dokwol-dong, Suncheon-si,
Cheonnam 540-744, South Korea
* Corresponding author (K.G. Song): Center for Water Resources Cycle Research, Korea Institute of
Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, South Korea; Tel.: +82 2
958 5842; fax: +82 2 958 6854; e-mail address: kgsong@kist.re.kr.
E-mail addresses: animaplus@hanmail.net (H.-C Kim); 213506@kist.re.kr (W.J. Choi);
carrit@naver.com (J.H. Ryu); andrew.maeng@gmail.com (S.K. Maeng); enrshs@hanmail.net (H.S.
Kim); biolee99@naver.com (B.-C. Lee)
Supplementary Information
1
SI-1. Biomass productivity and the maximum specific nutrient consumption rate (qmax) for
five different candidates incubated on pretreated piggery wastewater. Separate cultivation
experiments were conducted to select the best algal species among the examined algal species
using the optimal dilution factor. The initial color of growth medium (i.e., diluted SMR
effluent) was 588±6 mg Pt-Co L-1. The solution pH was adjusted to 8 prior to microalgae
cultivation, resulting in a decrease of pH level from 8 to 6.4±0.2 for all experimental
variations. For all statistical analyses, differences in the variables are considered significant at
the P <0.05 level of confidence.
Microalgae
species
a
Biomass productivity
(mg cell L-1 d-1) a
qmax
qmax
-1 -1 b
(mg-N g-cell d )
(mg-P g-cell-1 d-1) c
S. quadricauda
820±3
S. accuminatus
810±2
B. braunii
225±3
A. gracilis
370±1
C. vulgaris
480±1
P <0.05 (The P-value calculated by considering both
82±0.7
5.0±0.01
82±0.6
4.8±0.03
29±0.7
2.8±0.04
68±0.8
5.4±0.01
70±0.2
4.2±0.02
S. quadricauda and S. accuminatus is
0.109)
b
P <0.05 (The P-value calculated by considering both S. quadricauda and S. accuminatus is
0.129)
c
P <0.05 (The P-value calculated by considering both S. quadricauda and S. accuminatus is
0.010)
2