COMPARATIVE VERTEBRATE PHYSIOLOGY LAB, WWU BIOL 468, SPRING 2014 Lab Section: Instructor: Office Hours: Lecture Text: Recommended Text: CRN 23084, in BI 354, at 0900-1250, Fridays Dr. Roger Anderson In BI 311: M, T, W 0900-0945 open door Principles of Animal Physiology, 3rd Ed by Hill, Wyse, & Anderson Vertebrates: Comparative Anatomy, Function, & Evolution, 3rd Ed by Kardong Course Description: Design and implement laboratory research on some aspect of whole-animal performance physiology. Lab Objectives: You will participate in experiential learning, with respect to learning how to think and perform as a scientist, within the context of whole-animal physiology. You will perform non-invasive, non-injurious experiments with vertebrates. You may study ecologically relevant performance of animals in one of many potential performance criteria, such as running (acceleration, velocity, endurance), jumping, biting, and thermoregulating. You also may study the temperature-dependent performances of predator-evasion, predator-avoidance, bite force, bite velocity, learning, and cognition. You will be guided by Dr Roger Anderson in your (1) pursuit of a physiological question of interest on an ectothermic vertebrate of interest, (2) transformation of the question into testable, alternative hypotheses about cause & effect, (3) creation of a proposal to conduct experiments with available instrumentation and available animals to test assumptions and predictions of the hypotheses, (4) effort to develop reliable, systematic methods of analysis (5) pursuit of statistically analyzable data that either refutes or supports these hypotheses. (6) presentation of results audio-visually and in scientific paper format LAB SCHEDULE Introduction, choose project, set-up cages, stands, runways. Begin literature search to develop individual expertise that will contribute to team expertise. April 11 Begin to develop proposal (i.e., complete preliminary drafts of Introduction & Methods sections), 18 Complete first draft of proposal. Begin research set-up, learn to use instrumentation, submit proposal to another research team. 21 Submit review of another research team’s proposal. 25 Revise proposal and submit proposal (due after first hour of lab), solidify methods, write first complete draft of Introduction &Methods for team research paper (using student expertise from individual papers). 25 Obtain preliminary subject animals & perform & complete preliminary trials. May 2 Make final major changes in methods, obtain subject animals, perhaps begin data collection, entering & practice editing data, refine methods, begin re-writing Methods section of team research paper. 9 Begin or continue data collection, enter data, complete writing Methods section, Revise Introduction. 16 Continue or complete data collection, begin making tables, figures, and perform data analyses. 23 Complete data collection, perform or complete data analyses, complete Results section, revise Methods; make good progress on writing Discussion. May 27 Submit penultimate draft to another research team to review, begin developing power point presentation. May 30 Return edited draft; begin work on final draft of research paper and develop power point presentation. June 6 Give power point presentation, make final revisions, submit final draft (no later than noon June 2). April 4 Lab Assessment (150 points): 10 pts First Draft Team Research Proposal, due Friday, April 18 10 10 20 20 15 15 40 10 Team Proposal Review, due Wednesday April 21 at 1700 hours (individual & team assessment) Final Draft of Team Research Proposal, April 25 in lab (team assessment) Individual Paper, Physiological Research Techniques or Animal Subject Review, due Friday, April 18 Team Research Paper: penultimate draft, due noon Tuesday, May 27 (individual writing efforts assessed) Team Research Paper Review: due noon Thursday May 29 (with individual review efforts assessed) Team power point presentation, on Friday 1030, June 6 (individual & team assessments) Team research paper, Final Draft, due noon on Saturday, June 7 (team assessment) Individual assessed by other team members and Dr A for effort, comportment, and teamwork Individual performance and writing requirements: The physiological research “techniques paper”: 4-6 pages, 6 original references, minimum Team lab performance and writing requirements: The team laboratory research projects will result in team reports, both as papers and posters, written in scientific paper format. Explication of scientific paper writing and live presentations are well reviewed in Jan Pechenik's book titled A Short Guide to Writing About Biology. Copies are available to borrow from Dr. Anderson. The standards expected for the scientific paper are high, so a significant effort by all group members will be necessary. All data are to be entered into a spreadsheet, analyzed in a statistical package, and depicted in a graphics package, as well as written on a standard word-processing program. 2