Online Supplemental Material belonging to the manuscript: Increasing dietary protein intake does not preserve lean body mass during energy intake restriction in overweight older adults Evelien M.P. Backx1,2, Michael Tieland1,2, Karin J. Borgonjen-van den Berg1, Pauline R. Claessen1, Luc J.C. van Loon2,3, Lisette C.P.G.M. de Groot1,2 1 Department of Human Nutrition, Wageningen University, Wageningen, The Netherlands; 2 Top Institute Food and Nutrition, Wageningen, The Netherlands; 3 NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre+, Maastricht, the Netherlands. Summary: Macronutrient distribution and sample meal plan. Online Supplemental Material Table 1: Example of a daily meal plan* Food product High Protein diet Normal Protein diet Food product (g) 70 16 16 141 Breakfast Bread Cheese Meat Sweet topping Milk Food product (g) 105 32 17 160 Snack Cheese Supplement Bread 60 20 - Total meal protein amount (g) 33 Total meal protein amount (g) 17 16 25 35 35 9 Lunch Bread Cheese Meat Cream cheese Dessert 70 16 34 179 70 17 17 130 Snack Dessert - 130 Dinner Rice Meat Sauce Cooked vegetables Uncooked vegetables Dressing 129 80 45 150 100 85 70 45 150 100 32 16 14 15 12 35 17 Snack Supplement Dessert 20 - 25 75 Subjects decision of time to consume Low-fat margarine 20 25 Fruit (piece/portion) Egg (per week) 1 3 2 - Points from list with allowed food products 20 20 Other** 4 7 20 3 *Based on a subject with a body weight of 90 kg and an energy intake of 8 MJ/d (1911 kcal/d) during the intervention. **‘Other’ consists of the 10% of energy that the subjects could choose themselves from a restricted list, timing of those products was chosen by the subjects. Table 2: Macronutrients per meal in the Normal Protein group* Breakfast Snack Lunch Snack Dinner Snack Other ** Energy (kJ) 1417 972 1227 305 1546 678 1666 Energy (kcal) 339 232 293 73 369 162 398 Total fat (g) 10 5 8 1 16 4 9 Total carbohydrates (g) Total protein (g) 41 37 33 4 35 25 59 17 9 16 14 17 7 3 Fiber (g/d) 7 3 8 0 10 0 0 Alcohol (g/d) 0 0 0 0 0 0 7 * Based on a subject with a body weight of 90 kg and an energy intake of 8 MJ/d (1911 kcal/d) during the intervention. **‘Other’ consists of the 10% of energy that the subjects could choose themselves from a restricted list, timing of those products was chosen by the subjects. Table 3: Macronutrients per meal in the High Protein group* Breakfast Snack Lunch Snack Dinner Snack Other** Energy (kJ) 1938 955 1477 1911 417 1427 Energy (kcal) 463 228 353 457 100 341 Total fat (g) 16 9 9 15 0 10 Total carbohydrates (g) Total protein (g) 42 5 32 41 4 43 33 35 32 35 20 4 Fiber (g/d) 6 0 5 7 0 2 Alcohol (g/d) 0 0 0 0 0 8 * Based on a subject with a body weight of 90 kg and an energy intake of 8 MJ/d (1911 kcal/d) during the intervention. **‘Other’ consists of the 10% of energy that the subjects could choose themselves from a restricted list, timing of those products was chosen by the subjects.