1. FST 1201 BIOCHEMISTRY II
2. INSTRUCTOR
Mr. Abel Atukwase (BSc. Food Science and Technology, MSc Food Technology, PhD Candidate)
3. COURSE TYPE
Core for year 1 BSc. Food Science and Technology
4. COURSE STRUCTURE
Course credits (CU):
3 CU i.e. 3 contact hours per week per semester
Course duration: 15 weeks (45 h) – 30 lecture hours; 30 practical hours
5. COURSE DESCRIPTION
Brief introduction of biochemistry, Enzymology: overview of enzymes, properties of enzymes, classification and
nomenclature of enzymes, factors affecting enzyme activity, enzyme kinetics, enzyme inhibition; Metabolism and
bioenergetics: Introduction and definitions, laws of thermodynamics (1st and second law), metabolism and metabolic
control mechanisms of carbohydrates, lipids, proteins and amino acids, nucleic acids, vitamins and minerals.
6. COURSE OBJECTIVES
General objective
The course is designed to equip students with knowledge on the biochemical reactions that take place in the body
and the importance of such biochemical reactions in life
The specific objectives
i. To equip students with knowledge on enzymes mechanisms and how such mechanisms affect
metabolism
ii. To equip students with knowledge on how energy is produced, utilized and stored in the body
7. RECOMMENDED READING LIST
1. Bell, G.H, Smith D. E and Paterson, C. R (1976). Text book of Physiology and Biochemistry. 9 th Edition.
Churchill Livingstone, London
2. Conn, E.E and Stumpf, P.K (1976). 45th Edition. John Wiley & sons Inc.
3. Lehninger, A. L (1982). Principles of Biochemistry. Worth publishers, Inc. New York
4. Stryer, L. (1981). Biochemistry. 2nd Edition. W. H. Freeman and Company. New York
5. Christopher, K.M and van Holde, K.E, (1990). Biochemistry. The Benjamin/Cummings Publishing Company,
Inc. New York
8. 4. COURSE CONTENT, METHODS OF INSTRUCTION, TOOLS AND EQUIPMENT REQUIRED
TOPIC
1. Overview of enzymes
CONTENT





Definitions
Properties of enzymes
Classification and nomenclature of enzymes
Factors affecting enzyme activity
Effect of substrate concentration on enzyme
METHOD OF
INSTRUCTION /
Time allocated
Interactive lecture
(2 hrs)
TOOLS /
EQUIPMENT
NEEDED
Chalk /Markers
Blackboard/Whit
e board
LCD projector,
Computer
activity
Practical (3 hrs)
Functional lab,
glucose,
enzymes,
chemical
reagents,
practical handout
2. Enzyme Kinetics
 Definition of terms
 Derivation of the Mitchealis and Menten
equation
 Effect of temperature on enzyme activity
Interactive lecture Chalk /Markers
(2 hrs)
Blackboard/Whit
e board
Practical (3 hrs)
Functional lab,
potatoes,
practical handout
Enzyme Kinetics
 Importance of the Mitchealis and Menten
equation in enzyme kinetics
Interactive lecture Markers
(2 hrs)
Graph papers,
 Application of enzyme kinetics
Exercise (3 hrs)
 Effect of pH on enzyme activity
Practical (3 hrs)
3. Regulation of
enzymes
4. Bioenergetics and
Metabolism
 Enzyme inhibition
 Types of inhibitors (Reversible and
irreversible)
 Non protein biocatalysis
Pencils, pens,
graph papers
Fully functional
lab, liver,
practical
handout,
chemicals
Interactive lecture Chalk /Markers
(2 hrs)
Blackboard/Whit
e board
 Measuring the rates of enzyme catalyzed
reactions
Practical (3hrs)
 Comparison of different inhibition
mechanisms
Exercise (3 hrs)







Interactive lecture LCD projector,
(2 hrs)
Computer
Definitions
Importance of bioenergetics
Law of thermodynamics (1st and 2nd law)
Overview of thermodynamics
Overview of catabolism
Over view of anabolism
Free energy and chemical reactions
Exercise (3 hrs)
Fully functional
chemistry lab
Chemicals,
enzymes, starch,
practical handout
5. Carbohydrate
metabolism
 Definitions
 Glycolysis (aerobic and anaerobic)
 Tricarboxylic acid/Krebs cycle
 The Pentose phosphate pathway
Interactive lecture Chalk /Markers
(2 hrs)
Blackboard/Whit
e board
Practical (3hrs)
Fully functional
lab, chemical
reagents,
enzymes,
glucose, practical
handout
Lecture (2 hrs)
LCD projector,
Computer
 Gluconeogenesis
 Regulation of carbohydrate metabolism
Lecture (2 hrs)
Assignment
 Definitions
 Beta oxidation pathway
 Alternative oxidation pathways for lipids
 In vitro oxidation of fats
Interactive lecture Chalk /Markers
(2 hrs)
Blackboard/Whit
e board
LCD projector,
Computer, Flip
charts
Practical (3 hrs)
Fully functional
lab, fat, lipase
enzymes,
chemical
reagents &
practical
handouts
 Formation of ketone bodies and their
importance
Mid semester test
 Amino acid catabolism
 Urea cycle
Assignment (2
hrs)
2 hrs
Interactive lecture
(2 hrs)
 In vitro digestion of proteins
Practical (3 hrs)
 Fate of amino acid carbon skeletons
Assignment (2
hrs)
Interactive Lecture Chalk /Markers
(2 hr)
Blackboard/Whit
e board
LCD projector,
 In vitro glycolysis
6. Lipid metabolism
7. Protein and amino
acid metabolism
8. Nucleotide
metabolism
Metabolism of minerals
and vitamins
 Introduction
 Pathways in nucleotide metabolism
 Nucleotide biosynthetic routes
LCD projector,
Computer
Handouts
Question papers
Chalk /Markers
Blackboard/Whit
e board
LCD projector,
Computer, Flip
charts
Fully functional
lab, chemical
reagents,
enzymes,
practical
handouts
Handout
 Nucleic acid degradation
 Importance of nucleotide salvage
 Important vitamins in metabolism (vit A, B2,
Niacin)
 Important minerals in metabolism (calcium,
phosphorous, sodium, potassium )
9. SUMMARY OF TIME NEEDED
Lectures and assignments covering theory
Laboratory -based practicals
Class based exercises
30 hrs
21 hrs
9 hrs
10. OVERALL COURSE EVALUATION
Assignments
Class exercises
Practicals
Mid semester test
University examination
5%
5%
20%
10%
60%
Computer, Flip
charts
Lecture (2 hrs)
LCD projector,
Computer, Flip
charts
Interactive Lecture Chalk /Markers
(2 hrs)
Blackboard/Whit
e board
LCD projector,
Computer, Flip
charts