Subject name Physiological Aspects of Plant Resistance Subject code ENG.O.008 Department Department of Botany and Plant Physiology Faculty Faculty of Horticulture Subject supervisor/Lecturer Professor Maria Leja General information Objective and general description Lectures 5 x 2 hours Lab practicals 5 x 4 hours References Teaching period 1 semester / winter or summer semester ECTS credit 6 Lectures total 10 h Lab practical’s 10 h The subject comprises biochemical and physiological aspects of plant resistance to environmental (abiotic and biotic) factors. The special attention is paid to water, salinity, radiation, temperature and contamination stresses. The defence mechanism of plants against pathogens and pests (particularly insects) is presented as well as some problems of allelopathy. 1. Definition of stress, general reactions of plants to abiotic and biotic stresses, mechanisms of defense and tolerance to osmotic (draught & salinity), thermal, radiation, oxidative and environmental contamination stresses 2 hrs 2. General characteristics of plant resistance to pathogens. Passive and active resistance, induced mechanism of resistance, specific resistance, hypersensitivity reaction, structural barriers, phytoalexins, PR proteins. 4 hrs 3. Physiological aspects of allelopathy 2 hrs 4. Plant resistance to pests, biochemical interaction between plant and insects 2 hrs 1. Determination of proline in plant tissue, subjected to osmotic stress 3 hrs 2. Determination of phytoalexin (isocoumarine) in carrot roots infested by carrot fly. 4 hrs intopic 3 3. Measurement of lipid-protein membrane permeability in leaves of various plant species resulted from low temperature treatment 3 hrs 1.J.B. Harborne. Ekologia biochemiczna 1997 PWN 2.M.Kozłowska, G.Konieczny Biologia odporności roślin na patogeny i szkodniki. 2003. Wyd. AR w Poznaniu 3. A. Płażek. Patofizjologia roślin . 2011. Wyd. UR w Krakowie 4. J.B. Harborne. Introduction to Ecological Biochemistry. 1993. Academic Press Ltd. 5. Ashwani K.Rai & Teruhiro Takabe.Abiotic stress tolerance in plants. 2006. Springer.