Evaluation of GEM Germplasm for Resistance to Aflatoxin Contamination
Paul Williams and Gary Windham, USDA-ARS Corn Host Plant Resistance Research Unit, Mississippi State, MS
Forty-five single crosses, three-way crosses, inbred lines, and other GEM selections were evaluated in a
field test with three replications at Mississippi State in 2013. Two inbred lines, Mp313E and Mp719, and
two single crosses, Mp313E × Mp494 and Mp313E × Mp717, from the USDA-ARS breeding program
were included as resistant checks. B73 × Va35 was included as a susceptible check. Primary ears of the
plants in each plot were inoculated seven days after mid-silk with an Aspergillus flavus spore suspension
using the side-needle technique. The suspension was injected underneath the husks into the side of the ear
with a tree-marking gun. At maturity, ears were hand harvested, dried, and shelled. Grain was ground and
analyzed for aflatoxin concentration using the Vicam Aflatest procedure. The values for aflatoxin
concentration were transformed [ln(y+1), where y = concentration of aflatoxin in a sample] before
statistical analysis. Transformed means were compared by an LSD at P = 0.5. Geometric means were
calculated by converting the transformed means to the original units of measurement.
GEM germplasm evaluated for aflatoxin accumulation at Mississippi State in 2013.
Aflatoxin (ng/g)
PEDIGREE
INVENTORY# ln(y+1)
Geometric
Mean
(NEI9004:S2809-B-061-B-B/GEMS-0016)-B
CML413:N18-001-036-002-B
GEMS-0002
H8431/((GEMS-0115/Mp717)/TZAR101)
SCROGP3:N2017-172-001-B-B-SIB
((87916W/Mp717)/4676A)-B
BG070404:D27S42-B-014-B-B-SIB
((LH82/KO679Y)/GEMN-0205)-B
KUI44:N99am99bj
((794/KO679Y)/NEI9004:S2809-B-061-B-B)-B
PUER5:N99bb99bq
GEMN-0164
(((26e/GEMN-097)-SIB-SIB)/GEMN-0046)-SIB
(GEMS-0002/GEMS-0003)-B-B-062-B
PHJ40/((GEMS-0115/Mp717)/TZAR101)
CML341:S99ad99bg
Ki 14:S99ad99be
SANM126:N1299b-B-033-B-B-SIB
CML373:S99al43
B73 x Va 35
(CUBA164:S2012-459-001-B/GEMS-0002)-B-B-031-B-B-SIB
UR13085:S99g99u-B-025-B
PHJ40/(Mp717/KO679Y)
DK888:N11(95)-B-027-001-040-B-B-B
((LH61/KO679Y)/GEMN-0205)-B
(794/KO679Y)-B
794/((GEMS-0115/Mp717)/TZAR101)
(GEMS-0126/GEMS-0002)-B
(CUBA164:S2012-459-001-B/GEMS-0002)-B-B-011-B-B-SIB
12GEM03392
12GEM03405
09GEM07796
11GEM06393
09GEM07820
11GEM06410
12GEM03021
12GEM03397
12GEM80081
12GEM03394
12GEM80037
07GEM02612
12GEM09123
12GEM03060
11GEM06404
11GEM80039
11GEM80018
12GEM03026
11GEM80103
Susc. Check
11GEM06359
11GEM06074
11GEM06405
08GEM04784
12GEM03396
11GEM06419
11GEM06397
12GEM09089
11GEM06358
6.92
6.23
6.02
5.98
5.98
5.96
5.91
5.87
5.77
5.76
5.76
5.71
5.56
5.51
5.49
5.49
5.42
5.27
5.25
5.22
5.20
5.15
4.93
4.88
4.83
4.82
4.79
4.74
4.65
Aflatoxin (ng/g)
Page 1 of 2
1013
508
409
395
393
385
369
354
320
317
316
302
258
247
241
241
225
193
190
185
181
172
137
131
124
123
119
114
103
PEDIGREE
INVENTORY#
(GEMN-0196/GEMN-0205)/ANTIG03:N1242-B-007-B
DK888:N11(95)-B-027-012-009-B-B-B
CML311:S99aj99be
KUI44:S99al99bl
((KO679Y/CUBA117:S15-101-001-B-B-B-B-B-B-B)/GEMS-0016)-B
(GEMS-0030/CUBA117:S15-101-001-B-B-B-B-B-B-B)-B
DKXL380:N11-B-010-017-B-B-B
DK888:N11(95)-B-022-001-026-B-B
794/(Mp717/KO679Y)
(KO679Y/CUBA117:S15-101-001-B-B-B-B-B-B-B)-B
H8431/(Mp717/KO679Y)
DK888:N11(95)-B-003-001-001-B
DKXL370A:S11-B-028-001-B-B-Sib-B-B
DK888:N11-B-027-012-B-007-B
KO679Y
GEMS-0030/KO679Y
(GEMS-0002/GEMS-0003)-B-B-046-B
12GEM03979
08GEM04785
11GEM80017
11GEM80051
12GEM03393
12GEM09088
08GEM04769
Ames 31234
11GEM06398
11GEM06411
11GEM06396
08GEM05120
05GEM02813
11GEM00710
11GEM06555
12GEM04507
12GEM03059
Res. Check
Res. Check
Res. Check
Res. Check
Mp 313E x Mp 494
Mp 313E
Mp 719
Mp 313E x Mp 717
ln(y+1)
4.54
4.53
4.51
4.31
4.26
4.23
4.14
3.87
3.84
3.66
3.59
3.25
3.10
2.87
2.66
2.58
2.19
1.86
1.56
1.16
0.00
Geometric
Mean
93
91
90
73
70
68
62
47
45
38
35
25
21
17
13
12
8
5
4
2
0
1.65
LSD (0.05)
Plot means were transformed [ln(y+1), where y=aflatoxin concentration] before statistical analysis,
geometric means were calculated by converting transformed means transformed means to original units of
measure.
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