SYLLABUS COURSE TITLE FACULTY/INSTITUTE COURSE CODE DEGREE PROGRAMME FIELD OF STUDY ENVIRONMENTAL PROTECION COURSE FORMAT YEAR AND SEMESTER NAME OF THE TEACHER MOLECULAR MARKERS BIOTECHNOLOGY BIOLOGY AND AGRICULTURE IN ENVIRONMENTAL DEGREE LEVEL FORMA STUDIÓW/STUDY MODE I FULL-TIME LECTURES/PRACTICAL EXCERSISES/SEMINARS SUMMER LUKASZ JURCZYK PH.D COURSE OBJECTIVES THE AIM OF THIS COURSE IS AN INTRODUCTION TO MOLECULAR MARKERS TECHNIQUES, IDENTIFICATION, AND UTILIZATION IN ENVIRONMENTAL BIOTECHNOLOGY. THE COURSE PROVIDES AN OVERVIEW OF: TERMS, METHODS, AND PRACTICAL USE OF MOLECULAR MARKERS IN ENVIRONMENTAL PROTECTION. STUDENT WILL ACQUIRE KNOWLEDGE THAT CAN BE USEFUL BOTH IN FURTHER SCIENTIFIC CAREER DEVELOPMENT AND COMMERCIAL APPLICATIONS. PREREQUISITES LEARNING OUTCOMES NO. HOWEVER, AS IT IS A HIGHER DEGREE COURSE IN THE FIELD OF ENVIRONMENTAL PROTECTION, SO IT IS EXPECTED THAT STUDENTS IS KNOWLEDGEABLE ABOUT BASICS OF WASTEWATER TREATMENT, MICROBIOLOGY, ECOLOGY, GENETICS AND STATISTICS. KNOWLEDGE: AFTER COMPLETION OF THE COURSE STUDENT WILL BE ABLE TO: MENTION AND EXPLAIN DIFFERENCES BETWEEN MOLECULAR MARKERS DESCRIBE CURRENT TRENDS IN APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL SCIENCES PROPOSE AND DRAW A SCHEME OF RESEARCH METHODOLOGY IMPLEMETING MOLECULAR MARKER TO SOLVE SPECIFIC PROBLEM SKILLS: DETERMINE THE MOST SIUTABLE MOLECULAR TECHNIQUE FOR PERFORM LABORATORY ANALYSIS USING A CHOSEN MOLECULAR MARKER MEASURE, DETERMINE THE VALUES AND ASSESS ITS CREDIBILITY CHOOSE SOFTWARE AND METHOD TO PERFORM STATISTICAL ANALYSIS OF MOLECULAR DATA FINAL COURSE OUTPUT - SOCIAL COMPETENCES UNDERSTAND NEEDS OF APPLICATION MODERN METHODS IN ENVIRONMENTAL SCIENCES UNDERSTAND ENVIRONMENTAL THREATS CONNECTED WITH BIOTECHNOLOGICAL PRODUCTS AND TECHIQUES WORK INDEPENDENTLY AND IN A RESEARCH TEAM BE RESPONSIBLE FOR THE SAFETY DURING LABORATORY WORK RESPECTS THE PRINCIPLES OF SCIENTIFIC ETHICS IN COLLECTING AND DESCRIBING DATA COURSE ORGANISATION –LEARNING FORMAT AND NUMBER OF HOURS LECTURES – 30 HOURS LABORATORY EXERCISES - 25, FIELDWORK - 5 COURSE DESCRIPTION LECTURES INTORODUCTION, COURSE ORGANISATION, RULES OF COURSE ASSESSMENT, SEMESTER WORKS TOPICS REVIEW OF CHOOSEN GENETIC ISSUES OVERVIEW OF MOLECULAR BIOLOGY TECHNIQUES WITH A FOCUS ON PCR AS A KEY TOOL THE AREA OF INTEREST OF ENVIRONMENTAL BIOTECHNOLOGY THE CONCEPT OF MOLECULAR MARKER AND ITS APPLICATION, DETAILED OVERVIEW OF THE MAIN TYPES OF MOLECULAR MARKERS: CLASSES, TYPES, METHODS OF ANALYSIS PRACTICAL APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL BIOTECHNOLOGY: LAND BIOREMEDIATION PRACTICAL APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL BIOTECHNOLOGY: WATER RESERVOIRS REHABILITATION MONITORING PRACTICAL APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL BIOTECHNOLOGY: MUNICIPAL WASTEWATER TREATMENT PRACTICAL APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL BIOTECHNOLOGY: INDUSTRIAL WASTEWATER TREATMENT PRACTICAL APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL BIOTECHNOLOGY: MOLECULAR MARKERS IN NATURAL RESOURCES PROTECION PRACTICAL APPLICATION OF MOLECULAR MARKERS IN ENVIRONMENTAL BIOTECHNOLOGY: MOLECULAR MARKERS IN ECOTOXICOLOGY SAMPLING – PRACTICAL AND STATISTICAL ISSUES STORAGE AND PRESERVATION OF DIFFERENT TYPES OF RESEARCH SAMPLES STATISTICAL ANALYSIS OF MOLECULAR MARKERS DATA TOUR OF THE SEWAGE TREATMENT PLANT OR MUNICIPAL LANDFILL EXCERCISES THE EXTRACTION OF NUCLEID ACIDS FROM DIFFERENT TYPES OF SAMPLES PRACTICAL APPLICATION OF CHOSEN MOLECULAR MARKER: RAPD PRACTICAL APPLICATION OF CHOSEN MOLECULAR MARKER STR’S PRACTICAL APPLICATION OF CHOSEN MOLECULAR MARKER: DNA SEQUENCE MARKERS OVERVIEW OF AVAILABLE SOFTWARE ON FREEWARE PERFORMING STATISTICAL ANALYSIS BASED ON RECEIVED DATA METHODS OF INSTRUCTION REQUIREMENTS AND ASSESSMENTS Lecture supported with multimedia presentation, discussion, practical exercises, The written examination: test with open questions Excersises: continuous assessment of student’s activity, evaluation of semester paper. GRADING SYSTEM The prerequisite of the completion is positive assess of semester work and positive result of written exam according to evaluation table mentioned below. 93-100 % 85-92 % 77-84 % 69-76 % 60-68 % >60 % TOTAL STUDENT WORKLOAD NEEDED TO ACHIEVE EXPECTED LEARNING OUTCOMES EXPRESSED IN TIME AND ECTS CREDIT POINTS 93-100 % LECTURES EXCERSISES FIELDWORKS 5 4,5 4 3,5 3 2 HOURS 25 30 5 ECTS 1 1 - LANGUAGE OF INSTRUCTION INTERNSHIP MATERIALS PROJECT PREPARING PREPARING EXAMS TOTAL ENGLISH NO 15 0,5 FOR 15 0,5 90 3 PRIMARY OR REQUIRED BOOKS/READINGS: AVISE J., C. MOLECULAR MARKERS, NATURAL HISTORY AND EVOLUTION. KLUWER ACADEMIC PUBLISHERS 2001. FLETCHER H., HICKEY I., WINTER P., INSTANT NOTES IN GENETICS. TAYLOR & FRANCIS, 2012. EVANS G.,G., FURLONG J.,C,. ENVIRONMENTAL BIOTECHNOLOGY: THEORY AND APPLICATION JOHN WILEY & SONS, 2011 TCHOBANOGLOUS G., BURTON F.,L.,STENSEL H.,D. WASTEWATER ENGINEERING: TREATMENT AND REUSE. MCGRAW-HILL EDUCATION, 2003. SUPPLEMENTAL OR OPTIONAL BOOKS/READINGS: BALI G, RALLAPALLI, R., SUILLIA S.,B., SHIRALIPOUR A. KASUTRY S. ENVIRONMENTAL BIOTECHNOLOGY, APH PUBLISHING, 2002 DEWOODY A.,J. , BICKHAM J.,W., MICHLER C.,H. MOLECULAR APPROACHES IN NATURAL RESOURCE CONSERVATION AND MANAGEMENT. CAMBRIDGE UNIVERSITY PRESS, 2010 RUSSELL D., L. PRACTICAL WASTEWATER TREATMENT, JOHN WILEY & SONS, 2006. SINGH D., P., DWIVEDI S., K. ENVIRONMENTAL MICROBIOLOGY AND BIOTECHNOLOGY. NEW AGE INTERNATIONAL, 2004 SINGH A., WARD O.,P. APPLIED BIOREMEDIATION AND PHYTOREMEDIATION, SPRINGER, 2004