Electronic Supplementary Material
A Cu2(OH)3Cl-CeO2 nanocomposite with peroxidase-like activity, and its application to
the determination of hydrogen peroxide, glucose and cholesterol
Nan Wang, Jianchao Sun, Lijian Chen, Hai Fan*, Shiyun Ai*
College of Chemistry and Material Science, Shandong Agricultural University, Taian,
Shandong 271018, China
*Corresponding authors, Tel: +86 538 8247660, Fax: +86 538 8242251, E-mail address:
fanhai@sdau.edu.cn (H. Fan), ashy@sdau.edu.cn (S.Y. Ai)
Fig. S1 (A) pH and (B) temperature. 40 μg·mL-1 catalysts were used in 400 μL of 25 mM
phosphate buffer with 100 mM and 8 mM TMB as solution in this experiment.
Figure S1 showed that optimization pH and temperature are 3.0 and 40 °C, respectively.
1
Fig. S2 Steady-state kinetic assay. Double reciprocal plots of activity of material with the
concentration of one substrate (TMB or H2O2) fixed and the other changed. The velocity (V) of
the reaction was tested using 40 mg·mL-1 material in 400 μL of 25 mM phosphate buffer (pH
3.0) at room temperature.
Figure S2 showed that the slopes are similar and conform to the Ping-Pong mechanism.
Table S1 The data of linear equations and R2
TMB (mM)
H2O2 (mM)
equation
R2
2
Y=16.821x-1.520
0.965
4
Y=8.746x-0.052
0.973
8
Y=11.529x+37.264
0.909
8
Y=12.034x-9.098
0.999
10
Y=11.925x-12.180
0.991
12
Y=10.886x-12.184
0.985
2
Fig. S3 (A) Absorbance spectra of TMB reaction solutions catalyzed by material in the
presence of different concentration of glucose in 25 mM phosphate buffer (pH 3.0) at the room
temperature. (B) A dose-response curve for glucose detection. Inset: linear calibration plot for
glucose. (C) Absorbance spectra of TMB reaction solutions catalyzed by material in the
presence of different concentration of cholesterol in 25 mM phosphate buffer (pH 3.0) at the
room temperature. (D) A dose-response curve for cholesterol detection. Inset: linear calibration
plot for cholesterol. The error bars shown are the standard errors derived from three
measurements.
Figure S3 showed that glucose can be detected as low as 0.05 mM and the linear range was from
0.1 mM to 2 mM and cholesterol can be detected as low as 0.05 mM and the linear range was
from 0.1 mM to 2 mM.
Table S2 The data of equation and R2 of H2O2, glucose and cholesterol.
equation
R2
H2O2
Y=0.00797x-0.0137
0.996
Glucose
Y=0.00248x+0.00943
0.991
Cholesterol
Y=0.00243x+0.0016
0.990
3
Table S3 Figures of merit of comparable nanomaterials with peroxidase like activity for
H2O2.
Materials
Effects
of pH
Analytical
ranges
(mM)
LODs
(μmol L-1)
Methods
Reference
Cu2(OH)3Cl-CeO2
3.0
0.02-0.05
0.11
photochemical
-
magnetoferritin
8.6
5.8-88.2
9.63
electrochemical
[1]
AgNPs/PQ11/graphene
7.0
0.1-4
-
electrochemical
[2]
Table S4 Comparison of the kinetic parameters of Cu2(OH)3Cl-CeO2 and HRP
catalyst
Substrate
Km (mM)
Vm (10-8M/s)
Cu2(OH)3Cl-CeO2
H2O2
11.61
8.15
TMB
12.36
10.62
H2O2
3.99
8.71
TMB
0.434
10
HRP[3]
References
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