Electronic Supplementary Material
Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a
photometric enzymatic glucose assay
Caiping Dinga, Yinghan Yana, Dongshan Xiangb, Cuiling Zhanga,*, Yuezhong Xian a,*
a
Department of Chemistry, School of Chemistry and Molecular Engineering, East China
Normal University, Shanghai 200241, China
b
School of Chemical and Environmental Engineering, Hubei University for Nationalities,
Enshi 445000, China
*Corresponding
authors:
E-mail:
yzxian@chem.ecnu.edu.cn
(Y.Z.
Xian),
clzhang@chem.ecnu.edu.cn (C.L. Zhang) Tel.: +86-21-54340046; Fax: +86-21-54340046
Fig. S1 The photos of Fe3S4-MNPs dispersed in aqeous solution with concentrations
of 1 mg·mL-1 and 20μg·mL-1, respectively.
Fig. S2 The UV-vis absorbance responses for H2O2 detection using Fe3S4-MNPs as
enzyme mimetics.
Fig. S3 The UV-vis absorbance responses for glucose detection using Fe3S4-MNPs as
enzyme mimetics.
Fig. S4 UV-vis spectra of buffer solution (a) and 200-fold serial dilution of serum
sample (b) using Fe3S4-MNPs as enzyme mimetics. Inset: visualization photos of
colored product for buffer solution (a) and serum sample (b).
Table S1 Comparison of the apparent Michaelis–Menten constant (Km) and
maximum reaction rate (Vm)
Vm (10-8M s-1)
Km (mM)
Catalyst
Fe3S4
HRP[1]
Fe3O4[1]
Fe1−xMnxFe2O4[2]
ZnFe2O4[3]
TMB
H2O2
TMB
H2O2
0.160
0.434
0.098
0.139
0.85
1.158
3.7
154
310
1.66
1.146
10
3.44
4.5
13.31
2.168
8.71
9.78
3.639
7.74
Table S2 Comparison of various approaches for detection of glucose
Method
Electrochemistry
Electrochemistry
Fluorometry
Fluorometry
Colorimetry
Colorimetry
Colorimetry
Colorimetry
Detection limit of
Linear range of
Glucose (µM)
Gluocose (µM)
0.97
0.2
0.018
1.0
30
2.54
0.3
0.16
up to 2.04 103
0.4 - 1.2
103
0.05-50
3-100
50-1000
5-25
1.25-18.75
2-100
System
Co3O4
CuO/MWCNTs
Fe3O4/SiO2
CuO
Fe3O4 MNPs
H2TCPP-γ-Fe2O3
ZnFe2O4
Fe3S4 MNPs
Reference
[5]
[6]
[7]
[9]
[4]
[8]
[3]
This work
Table S3 Comparison of colorimetric detection of glucose based on the
peroxidase-like activity of different nanomaterials
Glucose sensing
system
MoS2 nanosheets,
GOx, TMB
WS2 nanosheets,
GOx, TMB
Graphene oxide,
GOx, TMB
Graphene oxideFe3O4, GOx, TMB
ZnFe2O4,
GOx, TMB
Bare Fe3S4
GOx,TMB
Reaction
time
Detection
limit (µM)
Linear
range (µM)
Reference
60 min
1.2
5-150
[10]
60 min
2.9
5-300
[11]
60 min
1
1-20
[12]
45 min
0.74
2-200
[13]
40 min
0.3
1.25-18.75
[3]
45 min
0.16
2-100
This work
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