Iris Hung
Bio 3B
Blood Type Lab
Report
By: Iris Hung
Block: 3B
Iris Hung
Bio 3B
Introduction:
The purpose, nature, and expected findings of the activity are to find the different types
of blood and calculate the red blood cells and white blood cells. We found the different
types of blood by observing the reaction, if agglutination appears we would know the
blood type. In the second part of the lab, we had to count the number of red and white
blood cells. There was no strategy for this, so the numbers we concluded with could be
inaccurate.
Hypothesis:
For the first part of the lab, we think that two out of three in each slide will agglutinate,
leaving one with no reaction. For the second part of the lab we predicted that the
amount of red blood cells would be much greater than the white blood cell.
Materials

4 Blood typing slides

12 Toothpicks

1 Microscope slide

1 Coverslip

1 Compound microscope (400x Magnification)

1 Marker

Mr. Smith Blood sample

Mr. Jones Blood sample

Mr. Green Blood sample

Mr. Brown Blood sample
Iris Hung
Bio 3B
Procedure Part A: ABO and Rh Blood Typing
1. Label the Blood slides: Mr. Smith, Mr. Jones. Mr. Green, and Ms. Brown.
2. Add three to four drops of each person’s blood in A,B, and Rh to every slide.
3. Add three to four drops of simulated anti-A serum in each A well for every slide.
4. Add three to four droops of simulated anti-B serum in each B well for every slide.
5. Add three to four drops of simulated anti-Rh serum in each Rh well for every
slide.
6. Stir each well with a clean toothpick for 30 seconds. Try to prevent splattering the
simulated blood.
7. Observe each slide and record your observations in Table 1. To confirm
agglutination try reading text through the mixed sample. If you cannot read the
text, assume you have a positive agglutination reaction.
8. Dispose the materials into the sink
Procedure Part B: Blood Cell Count
1. Shake one of the vials simulated blood and add one drop to a microscope
slide and then place the cover slip on. Make sure there are no air bubbles.
Iris Hung
Bio 3B
2. Find one square on the slide using lower power (10x) using the microscope.
3. Switch to high power (40x) and count the number of simulated red blood
cells inside the square. Record the number in the table 2 below.
4. Count the number of simulated while blood cell inside the square. Record the
number in the table 2 below.
5. Repeat this procedure in two other field of view.
6. Calculate the average of the three blood cell counts and the three white blood
cell counts.
7. Multiply the average number of red and white blood cell by the dilution
factor to determine the number of red and white blood cell per cubic
millimeter.
8. Dispose all materials in the sink.
Raw Data Presentation
Part 1: ABO and Rh Blood Typing
Iris Hung
Bio 3B
Part 2: Blood Cell Counts
Blood Sample: Mr. Jones
Anti – A Serum
Anti – B Serum
Mr. Smith
Agglutinated
Mr. Jones
Not
Agglutinated
Agglutinated
Not
Agglutinated
Agglutinated
Mr. Green
Ms. Brown
Not
Agglutinated
Blood Cell
Type
Cell Count
1
Red (Red)
White (Blue)
2
3
281 244 259
6
5
7
Processed Data Presentation:
Not
Agglutinated
Agglutinated
Anti – Rh
Serum
Agglutinated
Blood Type
Not
Agglutinated
B Rh-
A Rh+
A Rh+
Not
Agglutinated
0 Rh-
Total
# of
Cells
Average #
of Cells
Or Total/3
Dilution
Factor
784
261.3
150,000
Total # Blood Cells
per mm3 or Avg. #
of Cells x Dilution
Factor
1,9195,000
18
6
5,000
30,000
Iris Hung
Bio 3B
Blood Cell Count
Cell Counts
4
3
White (Blue)
2
Red (Red)
1
0
200
400
600
Number of Cells
800
1000
Conclusion:
After doing this activity my hypothesis was proven correct for the second part of the
lab. The first part of the lab we had to look at the reaction of each blood type. We
had to find the blood type of each blue by adding serum to it. If the blood had a
reaction this meant it was either Rh + or Rh -. The second part of the lab was to
count the red blood cells and the white blood cell. It turns out the number of red
blood cell is much greater compared to the white blood cell.
Evaluation:
The strengths we had in this lab were successfully identifying the different type of
blood. The weaknesses we faced were counting the number of red blood cells
because there were so many. Mistakes could’ve occurred by miscounting the
numbers. Some improvements could be would be getting a clearer image so it we
could count the number of blood cells better. We could also have also made more
observations and recounted the slides so our answers would be accurate.