Patrick A. Kudyba
Home: 6065 Tiger Trail Fort Worth, TX 76126
Email: Pak5193@psu.edu
Phone: +1-817-888-1543
EDUCATION
THE P ENNSYLVANIA STATE UNIVERSITY, UNIVERSITY P ARK, PA
THE EBERLY COLLEGE OF SCIENCE
B.S., expected May 2016
GPA: 3.21 (4.00 grading scale)
Major Specific GPA: 3.22 (4.00 grading scale)
Major: Biotechnology
Minor: Microbiology
EXPERIENCE
THE P ENNSYLVANIA STATE UNIVERSITY, S TATE COLLEGE, PA
Teaching Assistant
2015 Fall Semester
Department of Biochemistry and Molecular Biology
 Biotechnology 489 Animal Cell Culture Laboratory
 Equipped the laboratory for class-time procedures by setting up work stations, preparing chemical
compositions, and sterilizing surfaces and tools
 Supported students in carrying out the procedures and answered questions pertaining to the
laboratory steps and resulting data
Research Assistant
2014 Fall & Spring Semesters
The Animal Diagnostic Laboratory under S. Kariyawasam, BVSc, PhD, Diplomate ACVM, Diplomate ACPV
 Lab specialized in E. coli and Salmonella research
 Individual project involved the isolation and genotyping of E.coli found in White tail deer utilizing
both PCR and agarose gel electrophoresis methods to find specific genes within the E. coli samples
 Assisted in an additional project determining when antibiotic resistance first appears in Human
virulent E. coli using Kirby-Bauer disk diffusion assays
Orientation Leader for the International Student Orientation
2015 Fall Semester Program
The Undergraduate Office of Global Programs
 Lead three teams of 15-30 students in a week long orientation process involving engagement
activities and guiding information sessions regarding immigration and campus life
 Forge multicultural connections between students of varying backgrounds through directing teambuilding exercises
Orientation Assistant for the International Student Orientation
2015 Spring Semester Program
The Undergraduate Office of Global Programs
 Responsibilities included assisting international students with sign in, acquiring student IDs, setting
up bank accounts, providing education about the university and getting around campus
 Assisted in the execution of social and educational events to assist students with transitioning to the
culture of the United States and Penn State University
Examination Proctor
2014 Fall Semester
Department of Chemistry
 Proctored chemistry examinations for two different classes (CHEM 110 & 112)
 Responsibilities included presenting and collecting the exams from students and as well as upholding
academic integrity during the exam times
SKILLS
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Informed in animal cell culture techniques including:
Cell harvesting, counting and subculturing
T-antigen staining
Transformation by SV40 using the modified
calcium phosphate procedure
Primary cell culture using chicken embryo
fibroblasts
Proficient in plant cell culture methods comprising of:
Agrobacterium-mediated genetic
transformation
The use of the model system of Arabidopsis
Isolation and analysis of total plant genomic
data
RNA extraction and rtPCR from transgenic
plants
Trained in biofermentation processes such as:
Stock culture preparation
Protein expression and analysis
Measurement of cell growth rate
Media design
Cell growth in both BioFlo and RAMbio benchtop fermenters
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Skilled in microbiological applications for example:
Bacterial Growth Factors
Detection of rDNA
Semisynthetic Synthesis
Microarray Analysis
Biofilm Formation
Quorum Sensing in Bacteria
Attended Eppendorf clinic on detection of contamination in DNA and protein samples by
photometric measurements
Well-versed in polymerase chain reaction and gel electrophoresis techniques
Works well independently, as well as, in a team environment (e.g., collaborated with a team of 4 to
design experiments for an unknown microbe that we were charged with keeping alive for the
semester and identifying its species from a list of possible known bacteria)