SCI, Tissue culture, CSCR order form Aug 2013
Tissue Culture
WTCSCR
Tennis Court Road
Cambridge
01223 760293
PO Number
Date
Name
Tel number
e-mail
Group leader
Institute
Building
Collection/delivery
Feeder cells
Feeder
Cells/vial
Cost/vial
Active WT MEF
Active DS red MEF
Active DR4 MEF
Inactivated DS red MEF
Inactivated homozygous
DR4 MEF
~2-4x106
~2-4x106
~2-4x106
~2-4x106
~2-4x106
£4.50
£4.50
£42.00
£30.00
£30.00
Number
required
cost
Total
Growth factors
Growth factor
Cost £
bFGF2
Activin A
Actavin A-2
BMP2
BMP4
TAT-Cre
Ms LIF
Hu LIF
Ms FGF4
TAT-Dre
Chiron 1ml
Pd03 1ml
100
250
1000
200
250
50
400
500
160
50
8.39
8.67
500ug
250ug
2mg
250ug
250ug
50 ul at 0.5 mM
1mg
1mg
400ug
100ul at 0.2mM
Number
required
Aliquot size/composition
requested
Cost
Total
If you would like your GF making up and aliquoting please let us know. If you wish to buy less
than 1 stock item you can buy a little as one aliquot of our made up solutions- below
Reagent
Activin A
FGF2
Stock
250ug/vial
500ug
Aliquot
conc
0.5mg/ml
50ug/ml
Murine LIF
1mg/ml
10ug/ml
Human LIF
BMP 4
1mg/ml
250ug
10ug/ml
10ug/ml
Method
500ul 10mM HCL per 250ug vial
1vial into 10ml PBS
1mg vial to 100ml PBS (with
0.1%BSA)
1mg vial to 100ml PBS (with
0.1%BSA)
1 vial in 25ml 4mm HCL +0.1%BSA
Aliquot volume
5, 10, 15ul
25, 50, 80, 100ul
550ul
550ul
100ul
Serum
Serum
ES serum
ES serum 50ml
Cost each
£36.25
£3.70
Number required
cost
Total
N2B27
Reagent
Volume Cost each £
In house N2
5ml
33.50
Batch tested B27
Batch tested Neural
basal
10ml
500ml
40
31
Number
required
Total cost
total
Delivery
Addenbrooks
£11
Babraham
£15
Sanger
£20
For other locations please enquire about the cost
Please fill in this form and e-mail to Tissue-culture@cscr.cam.ac.uk
CSCR use
Total charges
Date
received
Date
Samples
set up
Samples set
up by
collection
date
Collection
signature
Recharge
raised