Supplementary Material
Investigation of SN2 [11C]cyanation for base-sensitive substrates: an improved
radiosynthesis of L-[5-11C]-glutamine
Tassilo Gleede†, ‡, Barbara Riehl†, ‡, Colleen Shea†, Lena Kersting†, ‡, Aylin Sibel Cankaya†, ‡,
David Alexoff†, Michael Schueller†, Joanna S. Fowler†, Wenchao Qu*, †
†
Biological, Environmental and Climate Sciences Department, Brookhaven National Laboratory,
Upton, NY 11973, USA
‡
Institut für Kernchemie, Johannes Gutenberg-Universität, D-55128, Mainz, Germany
Email: wqu@bnl.gov
Table of Contents
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Contents
Scheme 1. Diagram of [ C]HCN production procedure
Figure 1. Diagram of detailed Radiosynthesis process of L-[5-11C]-Glutamine
Figure 2. Representative HPLC analysis result of synthesized radiotracer L-[511
C]-Glutamine
Figure 3. Representative HPLC analysis result of Fmoc-[11C]Gln
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Scheme 1: Diagram of [11C]HCN production procedure.
Figure 1: Diagram of detailed Radiosynthesis process of L-[5-11C]-Glutamine.
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Quality analysis of L-[5-11C]-Glutamine
Figure 2. Representative HPLC analysis result of synthesized radiotracer L-[5-11C]-Glutamine.
Top: UV profile, L/D-Glutamine sample was co-injected with radiotracer sample for showing the
retention time of both L- and D-glutamine. Bottom: Radio-HPLC profile, it shows that the
synthesized radiotracer, L-[5-11C]-Glutamine, has high radiochemical and enantiomeric purities.
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Figure 3. Representative HPLC analysis result of Fmoc-[11C]Gln. Top: UV profile of Fmocderivatized sample. Bottom: Radio-HPLC profile.
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