Title: (Re)Making connections: linking migratory fish and tributary ecosystems in the Great Lakes basin Dr. Peter Mcintyre, UW-Madison, Center for Limnology Abstract: Migratory fishes are capable of generating large nutrient subsidies to their spawning grounds, but these effects are poorly understood for iteroparous species and at large spatial scales. Our research uses suckers as focal species for understanding how breeding migrations by native fishes connect ecosystem dynamics in the Great Lakes with their tributaries. We find that sucker migrations generate substantial inputs of nitrogen and phosphorus to small tributaries of Lake Michigan, and can be important even against a backdrop of moderate agricultural nutrient loading. These subsidies enhance nutrient availability, primary productivity, and insect growth in tributaries. Fish migrations are frequently blocked by dams and impassable road culverts, raising questions about the scale at which barrier removals would be necessary to restore ecological connectivity in the Great Lakes basin. Using an optimization model to maximize potential gains in spawning habitat from barrier removals, we find that restoration investments are most efficient when prioritized at large spatial scales and allocated in large pulses rather than sustained trickles of funding. Both dams and road culverts must be addressed to achieve efficient restoration. Together, these field studies and basin-wide analyses show that relatively modest restoration investments could accomplish major gains in ecological connectivity, thereby benefiting both native migratory species and the ecosystems in which they breed.