GUIDE TO SHAREPOINT SUBMISSIONS
Sharepoint is now used to log all samples for Mass Spec analysis.
HOW TO GET STARTED
-You must register to become a user of the sharepoint system. To do this, please e-mail Lisa Haigh
(l.haigh@imperial.ac.uk) with the following information:
Department
Supervisor
Cost Centre
Activity Code (if this is a grant please provide the start date of the grant).
-You will then be registered to use the system and sent a link to the online submission form.
ACCOUNTING
-When you are registered, you are registered to the Codes supplied above. If for any reason you
change supervisor (or move from MSc (or any other status) to PhD (or other) status) you must
inform me of the changes to allow for correct charging of the facility to PI’s. The facility will not be
held responsible for incorrect charging in this case.
LOGGING A SAMPLE
-When you receive the link to the Sharepoint site please follow the instructions below.
-Click on ‘Usage Records’
-Click ‘Make a booking’
-Complete the form that appears (Please see guidelines below).
-Click ‘Submit form’
-Your sample then appears on the ‘Usage Records’ page for analysis.
-Bring your clearly labelled sample over to Mass Spec (RCS1 Lab 112) and place the sample in the
white trays under the surname of your supervisor.
-If you fail to bring over a sample your booking will be deleted by the Manager after 2 weeks.
-You are not able to delete a booking once made. If you do need to delete your booking please
inform Lisa Haigh. Your booking will still appear on the system, but will not be run or the account
charged.
-Samples will be analysed and results emailed to the registered user.
HOW TO COMPLETE THE FORM
-Every individual sample must be logged even if you are using the Self-service (Open-access) option.
-The date is completed for you.
-Select your user name from the list of registered users.
-Add a sample reference. This must be the same as the labelled vial submitted to the lab.
-Technique. Please provide a preferred ionisation method. If none is selected, ES will be run as
default and possible resubmission required.
-Resubmission allowed? If this box is checked, it allows the facility manager to automatically
resubmit your sample for a different technique. If the box is not checked the results will be sent to
you (using the technique listed above) and the sample removed from the queue. It is then your
responsibility to make a new booking and provide a new sample.
-Return Sample? If checked, the sample will be placed in the return trays for you to collect. Samples
not collected within 2 weeks of submission will be disposed of. If not checked, samples will be
disposed of immediately (unless resubmitted for a different technique).
-Accurate Mass? Please check if you require accurate mass analysis.
-Service Type-Lab Service: The manager will run the sample on your behalf. Self-Service: If you
have been trained to use equipment in the Lab you can select this and run your own samples.
-Sample Structure: Please attach a file of the expected molecular ion. If no file is attached the
manager cannot be held responsible for the techniques run on sample. Files can be attached in
most formats (eg. .cdx, .doc, .PDF, .icl etc).
-Molecular Weight: This information must be provided.
- Formula: This information must be provided. Please note: If your sample is a salt, do not include
the salt in your formula (eg M.HCl salt, do not include the HCl in your formula,. Similarly, if your
molecule contains counter-ions do not include these in the formula (eg M+ Br-, or M+ PF6- do not
include the Br- or PF6- in the formula). Please also state if the sample is already charged. If you do
require the counter-ion data, please clearly specify this in the comments section.
-Solvent: Please select a suitable solvent for dissolvation of your sample from the list. If your
solvent is not listed then it is probably not suitable for Mass Spec analysis. If the solvent has a X next
to it (eg Chloroform (ESX)) then that solvent is not acceptable for that ionisation technique and will
not be used. In this case, chloroform is not suitable for ES analysis (chlorinated solvents can cause
ion suppression).
-Qty mg: Please provide accurate quantity of solid submitted. This is particularly important in ES
analysis for accurate dilutions.
-Qty mg/mL: If providing a solution, please ensure solvents used are LC grade, and again provide
accurate concentrations for accurate dilutions.
-Specify other solvent: You can specify an alternative solvent, but if not suitable for mass spec
analysis it will not be used.
-Hazards, please check as appropriate.
-Other Comments: Please add here any other information you feel is important. Also, please put
here if there are any buffers etc in your sample.
SAMPLE PREPARATION
Sample preparation can be very important in order for you to get the best analysis for your sample.
-Clearly label your sample.
-Make sure you have accurately specified the weight or concentration of your sample. This helps to
get the dilutions accurate for specific techniques.
-Always try to use the 2mL glass vials where possible. Vials with plastic lids can cause contamination
ions to appear in the spectra.
-If submitting a solution always use LC grade solvents. Non-LC grade solvents cause contamination
peaks to appear in your spectrum and cause contamination to the instrument.
-DO NOT use parafilm/nescofilm on your sample vials. This can cause contamination ions to appear
in your spectrum.
TURNAROUND
Samples submitted to the facility will be run as soon as possible, below I have listed typical
turnaround times (working days) for samples (please note these are only typical times and can vary
depending on quantity of samples submitted).
ES -1 day
LC (ES) 2-3 days
EI 5 days
CI 5 days
MALDI 5-6 days
LSIMS Only run when enough samples have been submitted and varies greatly.
GC (EI) Only run when enough samples have been submitted and varies greatly.
You will be informed if there is any delay/problem with the service in general by e-mail, or please
see the ‘service update’ section on the web page.