User
Office
Doc : SCI-USO-DOC-0067.doc
Issue : 1
Date: 8 May 2013
Page: 1 of 3
Report for beamtime received at Diamond funded by
BIOSTRUCT-X
Proposal ID and title
Project proposal no* and title
Principal Investigator for project (PI)
Principle scientific area
Secondary scientific area
Diamond visit reference number
Diamond visit date
Beamline used
Beamtime scheduled (hours)
Team members on visit
MX8492, Promoting human health by basic
structural and chemical biology research.
MX8492 is part of BIOSTRUCTX-6377.
BIOSTRUCTX-6377.1: Structural biology of
poly-ADP ribose polymerases
BIOSTRUCTX-6377.8: Egg-Sperm Interaction
at Fertilization
BIOSTRUCTX-6377.16: Structural studies on
arthritogenic autoantibodies
BIOSTRUCTX-6377.1 – Herwig Schüler
BIOSTRUCTX-6377.8 – Luca Jovine
BIOSTRUCTX-6377.16 – Doreen Dobritzsch
Protein crystallography
MX8492-20
6 March 2014
I03
5
Dr. Martin Moche (team leader)
Dr. Takako Saito
Elisa Dioguardi
Project Objectives
Short term:
Assess diffraction of crystals made from antibody-ligand complexes and membrane proteins involved
in egg-sperm interactions. Collect diffraction data on ligand complexes of poly-ADP ribose polymerase
proteins.
Medium term:
Determine the 3D structure of autoantibodies and poly-ADP ribose polymerases in complex with their
ligands and membrane protein structures involved in human fertilization.
Long term:
Improved understanding of autoimmune diseases, human fertilization and cancer.
User
Office
Doc : SCI-USO-DOC-0048.doc
Issue : 4
Date: 10/02/2016
Page: 2 of 3
*A biostructX Project proposal no. takes the form of the biostructx proposal id followed by the
project no. i.e.. BiostructX_proposal id: biostructx_1234 with two associated projects are
identified as 1234.1 and 1234.2 etc
Doc : SCI-USO-DOC-0048.doc
Issue : 4
Date: 10/02/2016
Page: 3 of 3
User
Office
Project Achievements for this Visit
6377.1
Tested several crystals and collected several datasets of various ligands - unfortunately only to around
3Å resolution. Refinement in progress.
6377.8
Confirmed membrane protein crystals however crystals diffracted to around 10Å at best.
6377.16
Test crystals resulted in a 4.2Å resolution dataset when auto-processed by Diamond. Crystals was
reprocessed with optimal settings and fewer frames since the later part of the data suffered from
radiation damage and structure of a mouse antibody in complex with a peptide has recently been
solved to 3.1Å resolution.
Beamtime received (in hours)
Signed by PI:
5
Print Name of PI:
Pär Nordlund (PI of MX8492) / Gunter Schneider (Lead-PI of BioStructX-6377)
Scan and email your completed form to useroffice@diamond.ac.uk