Online Supporting Information
“Single Nucleotide Polymorphisms in CETP, SLC46A1, SLC19A1, CD36, BCMO1, APOA5, and
ABCA1 are Significant Predictors of Plasma HDL in Healthy Adults.”
Authors: Andrew J. Clifford, Gonzalo Rincon, Janel E. Owens, Juan F. Medrano, Alanna J.
Moshfegh, David J. Baer, and Janet A. Novotny
Figures included:
Figure S1: Diet Folate Equivalent (DFE) histogram of Sacramento (n = 248) and
Beltsville (n = 505) study participants.
Figure S2: Red Blood Cell (RBC) folate concentrations histogram of Sacramento (n =
248) and Beltsville (n = 509) study participants.
Figure S3: Comparison of plasma vitamin B12 concentrations (pg/mL) in the
Sacramento and Beltsville populations.
Figure S4: Comparison of serum triglyceride levels in the Sacramento (n = 248) and
Beltsville (n = 505) study participants.
Figure S5: Comparison of total cholesterol (mg/dL) in the two study populations.
Figure S6: Comparison of HDL cholesterol (mg/dL) in the two study populations.
Figure S7: Comparison of LDL cholesterol (mg/dL) in the two study populations.
Figure S1
Comparison of dietary folate equivalent (DFE) intake assessed by the Block DFE Screener
(Information available at http://www.nutritionquest.com/assessment/list-of-questionnaires-andscreeners/) in the Beltsville (n = 506) and Sacramento (n = 248) populations. In the Beltsville
population, there were 257 female study participants (mean intake of 584 micrograms, mcg) and
253 male study participants (mean intake of 829 mcg). In the Sacramento population, there were
166 female study participants (mean intake of 826 mcg) and 82 male study participants (mean
intake of 765 mcg). The bimodal distribution of the Sacramento population may reflect the large
population of women of child-bearing age with high folate content in the diet.
Diet Folate Equivalent (DFE)
25
Beltsville (n=505)
Sacramento (n=248)
Percent
20
15
10
5
0
290 415 540 665 790 915 1040 1165 1290 1415 1540 1665 1790 1915 2040 More
DFE (mcg)
DFE
Beltsville
Sacramento
n
505
248
Mean
706
806
Median
628
766
Mode
618
Std Dev
352
424
Variance
123,919
179,685
Range
2852
1806
Figure S2
Comparison of red blood cell (RBC) folate concentrations (nmol/L) in the Sacramento and
Beltsville study populations that was analyzed using a previously validated LC/MS/MS method
(see ref 2 of manuscript for details). The very high levels of some of the RBC folate
concentrations may arise from high DFE intake (Figure S1) in some of the study participants. In
general, however, the RBC folate concentrations agreed well between the study populations.
RBC Folate (nmol/L)
20
18
Beltsville (n=509)
16
Sacramento (n=248)
12
10
8
6
4
2
0
385
485
585
685
785
885
985
1085
1185
1285
1385
1485
1585
1685
1785
1885
1985
2085
2185
2285
2385
2485
2585
2685
2785
2885
2985
3085
3185
3285
3385
More
Percent
14
RBC Folate (nMol/L)
RBC Folate
Beltsville
Sacramento
n
509
248
Mean
1435
1203
Median
1198
1161
Mode
854
987
Std Dev
819.1
273.7
Variance
670,932
74,928
Range
6585
1620
Figure S3
Comparison of plasma vitamin B12 concentrations (pg/mL) in the Sacramento and Beltsville
populations.
Plasma B12
25
Beltsville (n=506)
Percent
20
Sacramento (n=247)
15
10
5
0
115
230
345
460
575
690
805
920
1035
1150
1265
1380 More
Plasma B12(pg/mL)
Plasma B12
Beltsville
Sacramento
n
506
247
Mean
542
733
Median
505
661
Mode
441
397
Std Dev
244.5
293.1
Variance
59,757
85,928
Range
1733
1876
Figure S4
The mean (± standard deviation) concentration of serum triglycerides (mg/dL) was 92.7 (57.2)
for the Sacramento population and 103.4 (60.4) for the Beltsville populations. There were a few
participants with very high levels of serum triglycerides, include one study participant
(Sacramento) with a level of 461.0 mg/dL and another study participant with a level of 503.6
mg/dL (Beltsville).
Serum Triglycerides
25
Beltsville (n=510)
Sacramento (n=248)
Percent
20
15
10
5
0
40
60
80
100
120
140
160
180
200
220
240
260
280
300 More
TG (mg/dL)
TG
Beltsville
Sacramento
n
510
248
Mean
103.4
92.7
Median
88.0
77.5
Mode
45
49
Std Dev
60.4
57.2
Variance
3645
3271
Range
484
438
Figure S5
Comparison of total cholesterol values (mg/dL) in the Sacramento and Beltsville populations.
Total Cholesterol
20
18
Beltsville (n=510)
16
Sacramento (n=248)
Percent
14
12
10
8
6
4
2
0
120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 More
Total cholesterol (mg/dL)
Total Chol
Beltsville
Sacramento
n
510
248
Mean
180.6
200.2
Median
179.5
195.0
Mode
155.0
210.0
Std Dev
32.4
38.2
Variance
1045
1492
Range
203.5
233.0
Figure S6
Comparison of HDL cholesterol values (mg/dL) in the Sacramento and Beltsville populations.
HDL Cholesterol
20
18
Beltsville (n=510)
16
Sacramento (n=248)
14
Percent
12
10
8
6
4
2
0
30
35
40
45
50
55
60
65
70
75
80
85
90
95 100 More
HDL Cholesterol (mg/dL)
HDL
Beltsville
Sacramento
n
510
248
Mean
54.0
57.2
Median
52.5
55.0
Mode
55.0
55.0
Std Dev
13.0
14.1
Variance
169.0
200.2
Range
73.0
73.0
Figure S7
Comparison of LDL cholesterol values (mg/dL) in the Sacramento and Beltsville populations.
LDL Cholesterol
25
20
Beltsville (n=510)
Percent
Sacramento (n=248)
15
10
5
0
58
73
87
102 116 131 145 160 174 189 203 218 232 247 261 More
LDL Cholesterol (mg/dL
LDL
Beltsville
Sacramento
n
510
248
Mean
117.4
124.7
Median
115.8
120.0
Mode
110.5
107.0
Std Dev
28.8
33.7
Variance
827.0
1134.0
Range
197.0
218.0