Standard Operating Procedure
Settlement Class: Particularly Hazardous Substances
Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Department:
Chemistry
Date SOP was written:
1/8/2013
Date SOP was approved by PI/lab supervisor:
Principal Investigator:
Richmond Sarpong
Internal Lab Safety Coordinator/Lab Manager:
Lab Phone:
1/13/2013
Rebecca Murphy
510-643-2485
Office Phone:
510-643-6312
Emergency Contact:
Richmond Sarpong, 626-644-2407
Location(s) covered by this SOP:
Latimer Hall: 834, 836, 837, 838, 839, 842, 847,
849, 907
(Name and Phone Number)
(Building/Room Number)
Type of SOP:
Process
Hazardous Chemical
Hazardous Class
Purpose
This document describes the baseline requirements necessary for safe storage, use, handling and
disposal of chemicals that have been designated as “Particularly Hazardous Substances” (PHS) by
Cal/OSHA. This includes acute toxins, reproductive toxins, and select carcinogens.
Careful handling and stringent controls of these chemicals are essential in order to protect workers and
the environment. Requirements include designating a specific location for working with these substances
including labeling and warning signs for those who may enter or work in the designated areas.
Additional requirements may apply, depending on the specific chemical. Examples include particularly
hazardous substances that are also highly flammable and/or reactive.
Physical & Chemical Properties/Definition of Chemical Group
Particularly Hazardous Substances
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Particularly Hazardous Substances fall into three major categories: reproductive toxins, acute toxins,
and select carcinogens.
Reproductive Toxins are chemicals that affect the reproductive capabilities including causing
chromosomal damage (mutations) and adverse effects on fetal development (teratogenesis).
For a list of reproductive toxins, please consult the current List of Carcinogens and Reproductive Toxins
per Proposition 65 http://oehha.ca.gov/prop65/prop65_list/Newlist.html.
Acute Toxins are chemicals that pose a high level of immediate health risk to individuals. They can be
defined as:
1. A chemical with a median lethal dose (LD50) of 50 mg or less per kg of body weight when
administered orally to albino rats weighing between 200 and 300 g each.
2. A chemical with a median lethal dose (LD50) of 200 mg or less per kg of body weight when
administered by continuous contact for 24 hours (or less if death occurs within 24 hours) with the
bare skin of albino rabbits weighing between 2-3 kg each.
3. A chemical that has a median lethal concentration (LC50) in air of 5000 ppm by volume or less of
gas or vapor, or 50 mg per liter or less of mist, fume, or dust, when administered by continuous
inhalation for 1 hour (or less if death occurs within 1 hour) to albino rats weighing between 200
and 300 g each.
Select Carcinogens are a category of chemicals where the available evidence strongly indicates that the
substances cause human carcinogenicity. A “select carcinogen” meets one of the following criteria.
1. It is regulated by Cal/OSHA as a carcinogen.
2. It is listed under the category “known to be carcinogens” in the annual report by the National
Toxicology Program (NTP).
3. It is listed under Group 1 – “carcinogenic to humans” – by the International Agency for Research
on Cancer (IARC)
4. It is listed in either Group 2A or Group 2B by the IARC or under the category “reasonably
anticipated to be carcinogens” by the NTP, and causes statistically significant tumor incidence in
experimental animals in accordance with any of the following criteria:
a. After inhalation exposure of 6-7 hours per day, 5 days per week, for a significant portion
of a lifetime to dosages of less than 10 mg/m 3
b. After repeated skin application of less than 300 mg/kg of body weight per week; or
c. After oral dosages of less than 50 mg/kg of body weight per day.
Cal/OSHA Regulated Carcinogens fall into a separate hazard class and have extensive additional
requirements associated with them that are not covered under this procedure. The use of these agents
may require personal exposure sampling based on usage. The specific Cal/OSHA regulations that
govern the use of regulated carcinogens can be found at
http://www.dir.ca.gov/Title8/sb7g16a110.html
http://www.dir.ca.gov/Title8/5209.html
Some exemptions from these requirements are made for laboratory scale use; these can be found at:
http://www.dir.ca.gov/Title8/5191.html
Contact the EH&S department if there are any questions about the use of these materials.
Engineering Controls
Particularly Hazardous Substances
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
Work with particularly hazardous substances should be avoided and is not permitted if there is a
reasonable likelihood of workers exceeding regulatory exposure limits.

Chemical fume hoods used as containment areas for particularly hazardous substances must have a
face velocity of 100 lfm, averaged over the face of the hood and must be certified annually.

Laboratories and rooms where particularly hazardous substances are used shall have general room
ventilation that is at negative pressure with respect to the corridors and external environment. The
laboratory/room door must be kept closed at all times.
Personal Protective Equipment (PPE)
NOTE: Lab-specific information on PPE selection may be included in the Protocol/Procedure section.
Respiratory protection
NOTE: Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S.
This is a regulatory requirement.
Respirators should be used only under any of the following circumstances:
 As a last line of defense (i.e., after engineering and administrative controls have been
exhausted).
 When Permissible Exposure Limit (PEL) has been exceeded or when there is a possibility that
PEL will be exceeded.
 Regulations require the use of a respirator.
 An employer requires the use of a respirator.
 There is potential for harmful exposure due to an atmospheric contaminant (in the absence of
PEL)
 As PPE in the event of a chemical spill clean-up process
Hand Protection
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without
touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves
after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.
NOTE: Lab-specific and chemical-specific information on glove selection may be included in the
Protocol/Procedure section. Refer to glove selection from the link below:
For glove selection, go to: http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection-andusage.html
Particularly Hazardous Substances
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Eye Protection
Tightly fitting safety goggles. Use face shield (8-inch minimum) when appropriate (not protected by fume
hood sash for example). Use equipment for eye protection tested and approved under appropriate
government standards such as NIOSH (US) or EN 166(EU) or ANSI Z87.1.
Skin and Body Protection
Long pants, closed-toed and closed-heeled shoes, cotton-based clothing/attire, and flame resistant lab
coat must be worn for protecting against chemical hazards. Consider using a NOMEX garment if there is
a danger of fire.
NOTE: A NOMEX lab coat is recommended in the UC Berkeley Office of Environmental Health and
Safety document “Safe Use of Pyrophoric and Water Reactive Reagents”.
http://www.ehs.berkeley.edu/hs/126-standard-operating-procedures-sop.html
Hygiene Measures
Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at
the end of workday.
First Aid Procedures
Notify supervisor and EH&S immediately. Follow up with a call to 510-642-9090 to report the incident.
If Inhaled
Move person into fresh air. If not breathing, give artificial respiration. Immediately consult a physician.
In Case of Skin Contact
Take off contaminated clothing immediately. Wash off with soap and plenty of water for 15 minutes. Take
victim immediately to hospital. Consult a physician.
In Case of Eye Contact
Rinse thoroughly using an eyewash station with plenty of water for at least 15 minutes, occasionally lifting
the upper and lower eyelids. Get medical aid immediately.
If Swallowed
Do not induce vomiting unless the SDS recommends otherwise. Never give anything by mouth to an
unconscious person. Rinse mouth with water. Consult a physician.
Special Handling and Storage Requirements
NOTE: Lab-specific information on handling and storage may be included in the Protocol/Procedure
section.
Working Alone
Certain extremely hazardous operations should not be performed if the PI or Lab Safety Contact(s) are
not present. Never work alone with extremely hazardous materials/operations. See the
Protocol/Procedure section below for specific prohibitions (if any) on working alone.
Areas in the laboratory where PHS are used must be identified as a “Designated Area” for use. PHS
may only be used and stored in the designated areas. Additionally, be sure to label this area using
appropriate signage – e.g., CAUTION, DESIGNATED AREA – REPRODUCTIVE TOXINS, ACUTE
TOXINS, OR SELECT CARCINOGENS MAY BE PRESENT.
Particularly Hazardous Substances
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



Label waste containers containing PHS with warning labels.
Label storage space with warning labels.
Store non-flammable PHS within secondary containment.
Store flammable PHS within flammable storage cabinet and designate a bottom shelf or
secondary container.
Spill and Accident Procedure
Chemical Spill Dial 911 and 510-642-9090
Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors.
Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small area
using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution
tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic
bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 and 510-642-9090 for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S immediately. Follow
up with a call to 510-642-9090 to report the incident.
Chemical Splash Into Eyes – Immediately using an eyewash station rinse eyeballs and inner
surfaces of eyelids with water for 15 minutes by forcibly holding the eyes open. Seek medical attention.
Notify supervisor and EH&S immediately. Follow up with a call to 510-642-9090 to report the incident.
Medical Emergency Dial 911
Life Threatening Emergency, After Hours, Weekends and Holidays – Dial 911 or go
the nearest Occupational Health Center (Tang Health Center) or emergency room if it is after hours. Note:
All serious injuries must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to
report the incident.
Non-Life Threatening Emergency – Go to the nearest Occupational Health Center or emergency
room if it is after hours. Note: All serious injuries must be reported to EH&S within 8 hours. Follow up with
a call to 510-642-9090 to report the incident.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure,
flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse and then
enter your extension or go the nearest Occupational Health Center or emergency room if it is after hours.
Note: All needle stick/puncture exposures must be reported to EH&S within 8 hours. Follow up with a call
to 510-642-9090 to report the incident.
Particularly Hazardous Substances
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Decontamination/Waste Disposal Procedure
NOTE: Lab-specific information on decontamination/waste disposal may be included in the
Protocol/Procedure section.
Wearing proper PPE, decontaminate equipment and bench tops using procedures made in cooperation
with EHS&S. Please dispose of the water reactive and disposables contaminated with other water
reactives as hazardous waste.
Laboratory work surfaces shall be decontaminated at the conclusion of each procedure and at the end of
each day.
Decontaminate all equipment before removing them from the designated area.
Decontamination should be carried out in a glove box or fume hood.
Contaminated PPE must not be removed from the designated area until properly decontaminated.
Immediately after working with chemicals classified as PHS, remove gloves and wash hands/arms with
soap and water.
General hazardous waste disposal guidelines:
Label Waste
Label all containers with the label provided at http://ehs.berkeley.edu/hm/279-new-hazardous-wasteprogram-hwp.html. See the EH&S Fact Sheet, “Hazardous Waste Management” for general
instructions on procedures for disposing of hazardous waste.
Dispose of Waste


Dispose of regularly generated chemical waste within 6 months
Call EH&S for questions
Safety Data Sheet (SDS) Location
SDS can be accessed online at http://ucmsds.com
Particularly Hazardous Substances
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Particularly Hazardous Substances
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Documentation of Training (signature of all users is required)

Prior to conducting any work with particularly hazardous substances, designated personnel must
provide training to his/her laboratory personnel specific to the hazards involved in working with the
substance(s), work area decontamination, and emergency procedures.

The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a
copy of the SDS provided by the manufacturer.
I have read and understand the content of this SOP:
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01/02/2013