Additional file 1
Cellular responses induced by Cu(II) quinolinonato complexes in human tumor and
hepatic cells.
Zdeněk Trávníček1§, Ján Vančo1, Jan Hošek1, Roman Buchtík2, Zdeněk Dvořák3
1
Department of Inorganic Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of
Science, Palacký University, 17. listopadu 1192/12, CZ–771 46 Olomouc, Czech Republic,
zdenek.travnicek@upol.cz, phone +420 585 634 352. Fax +420 585 634 954
2
Department of Inorganic Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, CZ–771 46
Olomouc, Czech Republic
3
Department of Cell Biology and Genetics, Regional Centre of Advanced Technologies and Materials, Faculty of
Science, Palacký University, Šlechtitelů 11, CZ–783 71 Olomouc, Czech Republic
Table of Contents:
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Figure S1. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A549
cell line.
Figure S2. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the HeLa
cell line.
Figure S3. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the G361
cell line.
Figure S4. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A2780
cell line.
Figure S5. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the
A2780cis cell line.
Figure S6. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the LNCaP
cell line.
Figure S7. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the THP-1
cell line.
Figure S8. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the human
hepatocytes HH1.
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Figure S1. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A549
cell line. The cells were plated at 96-well dishes and cultured according manufacturer
instructions. The tested compounds were applied to the cells for 24 h in concentrations
ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The
cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and
each cytotoxicity experiment was repeated three times. The data are expressed as IC50  SD
(μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05) for 16 as
compared to cisplatin. The full symbols (■) and () indicate that the compound 5, and 6,
respectively, showed significant differences in the IC50 values (p < 0.05) to those compounds
marked with the corresponding empty symbols (□) for 2 and (○) for 14.
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Figure S2. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the HeLa
cell line. The cells were plated at 96-well dishes and cultured according manufacturer
instructions. The tested compounds were applied to the cells for 24 h in concentrations
ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The
cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and
each cytotoxicity experiment was repeated three times. The data are expressed as IC50  SD
(μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05) for 16 as
compared to cisplatin. The full symbols (■) and () indicate that the compound 5, and 6,
respectively, showed significant differences in the IC50 values (p < 0.05) to those compounds
marked with the corresponding empty symbols (□) for 13 and (○) for 13.
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Figure S3. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the G361
cell line. The cells were plated at 96-well dishes and cultured according manufacturer
instructions. The tested compounds were applied to the cells for 24 h in concentrations
ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The
cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and
each cytotoxicity experiment was repeated three times. The data are expressed as IC50  SD
(μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05) for 16 as
compared to cisplatin. The full symbols (■) and () indicate that the compound 3, and 4,
respectively, showed significant differences in the IC50 values (p < 0.05) to those compounds
marked with the corresponding empty symbols (□) for 5, and (○) for 1, 5 and 6.
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Figure S4. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the A2780
cell line. The cells were plated at 96-well dishes and cultured according manufacturer
instructions. The tested compounds were applied to the cells for 24 h in concentrations
ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The
cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and
each cytotoxicity experiment was repeated three times. The data are expressed as IC50  SD
(μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05) for 16 as
compared to cisplatin. The full symbols (■) and () indicate that the compound 3, and 4,
respectively, showed significant differences in the IC50 values (p < 0.05) to those compounds
marked with the corresponding empty symbols (□) and (○) for 5-6.
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Figure S5. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the
A2780cis cell line. The cells were plated at 96-well dishes and cultured according
manufacturer instructions. The tested compounds were applied to the cells for 24 h in
concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells
for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in
triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed
as IC50  SD (μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05)
for 16 as compared to cisplatin. The full symbol () indicates that the compound 4 showed
significant difference in the IC50 values (p < 0.05) to compound 2, marked with the
corresponding empty symbol (○).
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Figure S6. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the LNCaP
cell line. The cells were plated at 96-well dishes and cultured according manufacturer
instructions. The tested compounds were applied to the cells for 24 h in concentrations
ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The
cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and
each cytotoxicity experiment was repeated three times. The data are expressed as IC50  SD
(μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05) for 16 as
compared to cisplatin. The full symbols (), (■) and () indicate that the compound 4, 5, and
6, respectively, showed significant differences in the IC50 values (p < 0.05) to those
compounds marked with the corresponding empty symbols () for 1, (□) and (○) for 13.
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Figure S7. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the THP-1
cell line. The cells were plated at 96-well dishes and cultured according manufacturer
instructions. The tested compounds were applied to the cells for 24 h in concentrations
ranging from 0.01 to 50 M. The tested compounds were applied to the cells for 24 h. The
cytotoxicity was assessed by the MTT test, measurements were performed in triplicate, and
each cytotoxicity experiment was repeated three times. The data are expressed as IC50  SD
(μM). The symbol (*) denotes significant differences of IC50 values (p < 0.05) for 16 as
compared to cisplatin. The full symbols (#) (), (■) and () indicate that the compound 2, 4,
5, and 6, respectively, showed significant differences in the IC50 values (p < 0.05) to those
compounds marked with the corresponding empty symbols (#) and () for 1, (□) and (○) for
13.
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Figure S8. In vitro cytotoxicity of the tested complexes 16 and cisplatin against the human
hepatocytes HH1. The cells were plated at 96-well dishes and cultured according
manufacturer instructions. The tested compounds were applied to the cells for 24 h in
concentrations ranging from 0.01 to 50 M. The tested compounds were applied to the cells
for 24 h. The cytotoxicity was assessed by the MTT test, measurements were performed in
triplicate, and each cytotoxicity experiment was repeated three times. The data are expressed
as IC50  SD (μM). The symbol (*) denotes significant difference (p < 0.001) between the IC50
values for 2, 46 as compared to cisplatin. The symbol (>) shows that the measured
compounds were found non-toxic up to the concentration limit.
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