Supplementary material
Table S1. Sequence of primers used for Genotyping. The position of the primers along
the Stra6 locus are indicated in Fig. 1.
Figure S1. Three-dimensional OCT (3-D OCT) rendering of retinal and ocular
structures. A series of 100 b-scans were collected, then stacked and spatially aligned to
form a registered three-dimensional (SD) rendering of retinal volume. A: asteroid-like
structure. B: blood vessels. C: optic nerve head. D: posterior surface of lens. E:
Shadow cast by asteroid-like structure. Notice the close proximity of this structure with
respect to the lens.
Figure S2. Western blot analysis showing the comparison between the three Stra6
genotypes Stra6 +/+, Stra6 +/-, and Stra6 -/- for the expression of relevant proteins from
the visual cycle namely RPE65, LRAT and RGR. For all three genotypes, no difference
was observed in the level of expression of each protein analyzed. No Stra6 protein was
detected in the Stra6 -/- extracts. The size of immunodetected proteins is indicated in
parentheses.
Figure S3
Fundus and SD-OCT images from 6 week old Stra6 mice. A and B are fundus images
of a Stra6 -/- null mouse showing the presence of a PHPV in the right eye (B). C and D
are OCT images of both eyes showing the presence of a dense structure only in the
1
right eye (D). E and F show the comparison of visual responses between WT with Stra6
-/- null mice. Visual responses for OD and OS of null mice were similar (F), but reduced
when compared to WT (E).
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Table S1
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Figure S1
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Figure S2
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Figure S3
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