Budget Justification Sample
(None of the names used here is real; please do not infer them to anyone in reality.)
PERSONNEL: $$$$/yr for 2 yr
Senior/Key Personnel
Scott Smart, Ph.D. (Principal Investigator, 1.2 months/yr. Dr. Smart is a DoD employee, and no salary is
required) is responsible for directing the research of the laboratory personnel, for editing and submitting
progress reports to the DoD, for editing and submitting research papers to scientific journals, and for ensuring
compliance with all relevant regulations. He has a strong history of funding and awards in cancer research,
including a Leukemia and Lymphoma Society Special Fellowship and a Kimmel Scholar Award from the
Sidney Kimmel Foundation for Cancer Research. He has also obtained grants from the Program in Brain and
Immuno-Imaging from the Dana Foundation, and an R01 from the National Institutes of Health. He has
published his work in well-respected journals, including Immunity, PNAS, Molecular Biology of the Cell,
Journal of Biological Chemistry, and the Journal of Immunology. Dr. Smart has over 20 yr experience with
molecular biology, promoter analyses, and mammalian expression vector design. His research is focused on
lymphocyte biology and NF-κB signal transduction, and he has been studying the Bcl10 protein and its
interacting partners for over a decade. His group identified the DOBERMAN protein and characterized its
activity. Therefore, the PI clearly has ideal qualifications to supervise the studies described in this proposal.
*Noel Wright, Ph.D. (Research Associate, 6 months/yr) will perform the experiments in this proposal, with
assistance from Mr. Help. She received her Ph.D. in Biology from the Bulgarian Academy of Sciences and
post-doctoral training in Biophysics at the Bulgarian Academy of Sciences, the Roswell Park Cancer Institute,
and at Duke University. Dr. Wright has a strong publication record and training in diverse areas of
biochemistry and cell biology. In her time with the PI, she has developed substantial expertise in the
maintenance, transfection, and flow cytometry analysis of lymphocyte cell lines, as required for this proposal.
Dr. Wright is employed through the Henry M. Jackson Foundation. She has worked for the PI for 6 years, and
has contributed substantially to many of the PI's ongoing and published studies.
Other personnel
*Alan Okay (Research Assistant, 6 months/yr) will perform DNA cloning, sequencing, and mutagenesis to
support Dr. Wright’s experiments. He will also provide general technical support, performing routine tissue
culture, ordering reagents, and maintaining databases of cell lines, plasmids, and oligonucleotides, as
required for the project. Mr. Okay is employed through the Henry M. Jackson Foundation. He has worked
for the PI for 5 years, and is very skilled with molecular biology techniques and tissue culture.
TBD, Postdoctoral Fellow (12.0 calendar months/yr), will be responsible for cultivation of recombinant
HHV-8, development of cell culture assays involving immunofluorescence (IFA) as well as transcriptional
profiling of gene expression using real-time and quantitative PCR, generating new cell lines expressing a
variety of cellular molecules predicted to play a role in the inflammatory axis in order to study them in
isolation. The fellow will be encouraged to write manuscripts based on novel findings from the proposed
work. This individual will be hired as a Henry M. Jackson Foundation employee.
EQUIPMENT:
Xxxxxxxx (please see attached quote.)
TRAVEL: $SSSS/YR
One person the PI will to attend a DoD research-related meeting at xxx location, budget will cover flight, hotel,
per diem, conference registration cost.
OTHER DIRECT COSTS
Materials and Supplies:
The supply budget reflects the requirements for the proposed research. These estimates are based on current
prices and projected quantities of the materials requested in the supply budget.
Cell culture media and sera: $4,000/yr.
We will require endotoxin-free fetal calf serum, currently priced at $150/bottle, and we expect to use an
average of 15 bottles/yr, which gives a total for serum of $2,250/yr.
Tissue culture media: RPMI is obtained at a price of $10 per 500 mL bottle, and we will use 150
bottles/yr at a cost of $1500/yr
Other miscellaneous tissue culture supplements will cost approximately $250/yr.
Total for cell culture ($2,250 + $1,500 + $250) = $4,000
Disposable plastics and glassware: $9,500/yr.
We will require supplies for tissue culture, including disposable tissue culture pipets, tissue culture
flasks and multi-well plates. ELISA plates will be needed for cytokine ELISAs. In addition, we
will need petri dishes for bacterial growth, syringes and needles, 50 mL, 15 mL, 4 mL and 1.5 mL
disposable tubes, PCR tubes, FACS tubes, polystyrene tubes with snap caps, disposable pipet tips
(with and without aerosol barriers), filter units for sterilization of tissue culture solutions—these are
essential supplies for molecular biology, bacterial growth, centrifugations, storage of solutions,
plasmid and protein purifications, mouse immunizations, etc.
Disposable tissue culture pipets (25 mL, 10 mL, 5 mL, 2 mL, 1 mL): $1,500/yr Disposable tissue
culture flasks (162 cm2, 75 cm2, 25 cm2): $3,000/yr Multi-well plates (24-well, 96-well): $1,500/yr
Sterile, disposable tubes (1.5 mL, 4 mL, 15 mL, 50 mL, 1 mL Titertubes): $2,000/yr Sterile aerosol
barrier pipet tips: $500/yr Miscellaneous (Multi-channel pipet basins, Petri dishes, sterile filtration
units, syringes, needles):
$700/yr Miscellaneous Glassware: $300/yr Total for Disposable plastics and glassware ($1500 + $3000
+ $1,500 + $2000 + $500 + $700 + $300) = $9,500/yr
Publication Costs: $1,000/yr.
We estimate average publication costs of $1,000/yr to report the data produced via execution of the proposed
research plan.
Consultant Services Costs: $$$$/yr
Two consultants will be hired at $xxx/hour rate and total xxx hours for the entire project. Their
responsibilities will be xxxxx.
FY 2014 Indirect Costs and Fringe Benefit Rates:
* The fringe rate used is 33.43% for Tier 1 employees and 3.60% for Tier 2 employees (all employees in
this proposal are Tier 1).
HJF indirect cost (IDC) is calculated based on the value-added cost base overhead rates. The 36.48% of USU
on-site rate is applied on for all allowable direct cost, less sub-award costs; additional 14.30% of
Company-wide G&A rate is applied on the total direct cost less sub-award. For proposals including
subawards, additional 1.23% is applied on total sub-award costs. These fringe benefit and indirect cost rates
were proposed to U.S. Army Medical Research Acquisition Activity as provisional rates on 9/16/2013.
Please see attached quote for equipment.