2016 Summer Research Program for DDS and DH Students Projects Supervisor(s) Project Abd-Elmeguid, Ashraf Title: Volumetric analysis of pulp space and descriptive analysis of calcified root canals. Status (Open, *Pending or Filled) Open Abd-Elmeguid, Ashraf Title: Effect of different Irrigants on the preparation of calcified root canal. Open Alsufyani, Noura Open Alsufyani, Noura Amin, Maryam Amin, Maryam Amin, Maryam Amin, Maryam; Ganatra, Seema Elahi, Shokrollah Title: Systematic review of Cone beam CT incidental findings of the cervical spine and clivus Brief summary: The aim is to systematically review the literature to document the number and nature of incidental findings noted in the cervical spine and clivus. These extragnathic sites represent challenging differential diagnosis for the expert and novice radiologists. Title: Accuracy of Upper airway segmentation using low resolution cone beam CT: A pilot study Brief summary: Using a semi-automatic segmentation program, the aim is to compare the upper airway reconstruction using low-moderate resolution cone beam CT. The results of this study may impact the CBCT protocol for upper airway analysis to reduce radiation dose. Title: Immigrant Parent's Sense of Coherence and Children's Oral Health Status and Related Behaviors. Brief summary: The aim of this community-based cross-sectional study is to explore the associations between parents/caregivers coping skills and oral health status and oral health-related behaviors of their children among recent immigrant families in Edmonton. Title: Patient's compliance and success of orthodontic treatments. Brief summary: The aim of this retrospective clinical project is to assess the association between four important behaviors related to orthodontic treatment compliance (oral hygiene, appointment punctuality, appliance wear, and appliance maintenance) and the successful outcome of the treatment (achieving promising treatment outcomes after the anticipated treatment time, stability of the treatment). Title: Partnership for Prevention: Integrating Preventive Oral Health Measures into Pediatric Primary Care for First Nations Children. Brief summary: The overall goal of this project is to establish a collegial partnership between medical and dental providers and a First Nation community in order to successfully integrate preventive oral health measures into a Pediatric Outreach Clinic in the Maskwacis Health Centre. Title: A Comprehensive Oral Health Needs Assessment among Underprivileged Communities in Edmonton. Brief summary: This cross-sectional study aims to comprehensively assess unmet dental, periodontal, and oral mucosal health needs of underprivileged communities in Edmonton, with a special focus on screening for pre-cancerous and cancerous lesions of oral mucosa. Title: Investigating the cross talk between CD71+ erythroid cells and regulatory T cells. Brief summary: In this project the student will use tissue culture assays and flow cytometry techniques to determine the cross talk between Open Open Open Open Open Open CD71+ erythroid cells and regulatory T cells using newborn mice splenocytes. Elahi, Shokrollah Eggert, Michael Febbraio, Maria Febbraio, Maria Flood, Patrick Flood, Patrick Flores-Mir, Carlos Title: Investigating the immunological factors associated with the development of inflammatory bowel disease (IBD) in newborns. Brief summary: In this project the student will use tissue culture assays, flow cytometry and PCR techniques on cells obtained from mothers with IBD compared with non-IBD mothers to determine possible predisposing mechanism of IBD induction in their offspring. Title: Clinical Demographics of Periodontal Patients attending the UofA School of Dentistry. Title: Does CD36 signaling impact fatty acid uptake and esterification? Brief summary: In this project the student will use tissue culture assays to determine whether CD36 alters fatty acid uptake & esterification. Fatty acids are critical to energy and construction of cell membranes and organelles, but we still don’t understand how they enter cells. This project will provide the student with opportunities to learn basic cell biology techniques and address a mystery in our understanding of fatty acid biology. Title: Creation of CD36 mutants by site directed mutagenesis. Brief summary: Have you ever wanted to be a genetic engineer? This is your chance-in this project you will use cutting edge molecular biology techniques to engineer specific mutations in CD36 by site-directed mutagenesis. These will then be expressed in tissue culture cells and tested for their ability to perform CD36 functions. Those that no longer take up fatty acids but can do all other CD36 functions will be used in future studies. Title: Inhibition of the oxidative stress response induced by LPS from Periodontal pathogen Porphyromonas gingivalis by B2-Adrenergic Receptor agonists. Brief Summary: B2-Adrenergic receptor agonists are used as inhalants to treat Chronic Obstructive Pulmonary Disease (COPD), and it has been found that they are strong anti-inflammatory drugs which can inhibit other chronic inflammatory conditions in patients who regularly use them. Periodontal Disease (PD) is a chronic inflammatory condition of the oral cavity, and is caused by an inflammatory response to the biofilm infection of the periodontal pocket. This aim of this project is to determine if b2-AR agonists can inhibit the oxidative stress response in inflammatory cells induced by Porphyromonas gingivalis (Pg), one of the major Periodontal pathogens thought to cause PD . Title: Conversion of a pro-inflammatory to an anti-inflammatory response by NF-kB inhibitors. Brief Summary: NF-kB inhibitors have been used to successfully treat chronic inflammatory conditions such as IBD, cardiovascular disease, Parkinson’s disease, and cancer in animal models of these human diseases. Periodontal Disease (PD) is a chronic inflammatory condition of the oral cavity caused by an inflammatory response to the pathogen bacteria. One of the most common ones identified as causing PD is Porphyromonas gingivalis (Pg), which produces an endotoxin that is highly inflammatory. The aim of this study is to determine if a small molecule pharmaceutical NF-kB inhibitor can inhibit the production of proinflammatory cytokines and enhance the production of anti-inflammatory cytokines in Pg endotoxin-stimulated macrophages. Title: “Patient’s experiences at 1 week, 1 month and 2 months using fixed class II correctors delivered through two distinct treatment protocol”. Open Pending Open Open Open Open Open Graf, Daniel Graf, Daniel Lagravere, Manual Lagravere, Manual Lagravere, Manual Levin, Liran Levin, Liran Levin, Liran Lockwood, Sally Martinez, Ysidora Torrealba Martinez, Ysidora Torrealba Martinez, Ysidora Torrealba Pachêco-Pereira, Camila; Flores Mir, Carlos Passos, Sheila Brief Summary: Surveys have been collected as part of randomized clinical trial. Title: How do Bmp signals shape the adult tooth-alveolar bone complex Description: This project can accommodate 2 students. Bone Morphogenetic Proteins (BMP) are important regulators of tooth development. Their contribution to tissue homeostasis in adult teeth is less studied, though evidence supports their involvement in orthodontic tooth movement. In this project we investigate the tooth-alveolar bone complex in BMP mutant mice to define changes to in enamel, dentin, cementum, periodontal ligament, and roots. Techniques employed are histology, immunohistochemistry, µCT. Title: Skull formation in compound BMP2/7-deficient mice Description: Bone Morphogenetic proteins are important regulators of bone formation. Loss of Bmp2 and Bmp7 affects size, shape, and mineralization of the calvaria. We will characterize differences in shape and mineralization (Alizarin red staining, µCT) of newborn mice. We will set up calvaria explant cultures and calvaria osteoblast cultures to study differences in osteoblast differentiation and bone mineralization. Techniques involve skeletal preparations, microscopy, dissection oof calvaria, tissue culture, qPCR. Title: Analysis of Cone-beam computer tomographies (CBCT- 3D images) Title: Analysis of Three-dimensional facial scans Title: Analysis of 3D model print outs Title: Long term evaluation of implants and teeth survival and success. Title: Review of hot topics in periodontology. Title: The use of protective mouth-guards among hockey players. Title: Using video clips to support learning in preclinical dental hygiene education Description: The objective of this research is to investigate the use of video clips as a learning resource in preclinical dental hygiene education. Over the past few years, videos have been developed in an attempt to add a valuable learning resource to the preclinical program. The intent of this study is to gather feedback from the students in order to support further development of teaching materials and to discover whether the teaching video clips are a useful addition to the course content resources. Specifically, are the videos useful in helping the student achieve their learning objectives? Part of the summer project will include ongoing development of videos to be added to the learning resources for this pre-clinical Dental Hygiene course. Open Open Open Open Open Open Open Open Open Title: Accuracy evaluation of a 3-D educational software. Open Title: Comparison of the 3-D digital impression and 3-D print out model. Title: Evaluation of different CAD-CAM digital restorations. Open Title: Patient Satisfaction after orthodontic treatment. Description: This survey is in place and the data has been collected since July/2015. The student commitment will be related to data analysis, interpretation of results and article draft. Flexible schedule during summer time. Title: Understanding the microstructural bonding mechanisms between zirconia and porcelain Open Open Open Passos, Sheila Senior, Anthea; Ganatra, Seema; Alsufyani. Noura; Pachêco-Pereira, Camila Senior, Anthea; Ganatra, Seema; Alsufyani. Noura; Pachêco-Pereira, Camila Yoon, Minn Title The effect of heating procedures during fabrication of zirconiabased restorations. Title: Retake rates in oral radiology Description: This project will present frequency of the most common reasons of clinical retakes on an undergraduate dental school. Open Title: Patient safety in oral radiology, specifically promoting the use of rectangular collimator in private practices. Pending Pending Title: TBD *Projects will be marked pending when a student has informed Heather Good that a supervisor has accepted them for a project, but the paperwork is not yet complete. For more information, please contact Heather Good at 780-492-4469 ([email protected]) INFORMATION FOR SUPERVISORS For the summer of 2015, the SoD provided financial support for up to three students per supervisor. If a supervisor had more than three students they had to guarantee financial support for the extra student(s) from their own funds. We hope that the SoD will provide a similar level of support in 2016 however we are waiting for confirmation of the level of support that will be available. Each student should have their own project. A detailed description of the project will need to be provided before any students will be signed up for your project. The required information will include details about the training requirements for the student; details about the type of certification or ethics approval required; and the status of the certification (a from will be provided). If certification or ethics approval is required for your project(s), proof of this approval is required before a letter of offer will be formalized with the student. Every supervisor is expected to report annually a list of the publications, presentations, and awards related to their students’ summer research projects. INFORMATION FOR STUDENTS We anticipate that the School of Dentistry (SoD) will provide some funding for University of Alberta (UofA) DDS and DH expected to continue in their DH or DDS program in the fall of 2016. Each UofA DDS or DH student was guaranteed up to 100 hours of financial support. Note: There will be opportunities for other undergraduate students and volunteers, however, they should contact [email protected] or [email protected] for more information. Student researchers are expected to have completed the appropriate safety and/or ethics training required for their project before starting their project. The time required to complete this training will not be paid for by the School. A measureable outcome from this research program is expected from each summer research project. The outcome expected could be in the form of publication and/or a presentation at a national or international conference. The School will set aside $500.00 per student for travel funding, and students are also encouraged to apply to The Fund For Dentistry for a Student Travel Award for additional funding if required ($500.00 maximum award per student). Student researchers are expected to give an oral and written progress report at the end of the Summer Research program. Student researchers are expected to attend monthly noon-hour research meetings over the summer.