AF5
BEST BASIC SCIENCE POSTER AWARD FINALIST
Fabrication, Characterization And Human Mesenchymal Stromal Cell
Attachment Property Of A Novel Tricalcium Phosphate-Chitosan-Fucoidan
(TCP-Ch-Fu) Biocomposite Scaffold For Bone Tissue Engineering Application
Subramaniam Puvaneswary, Sepehr Talebian, Hanumantha Rao Balaji Raghavendran, Malliga
Raman Murali
Background: Biocomposites have been widely used to create scaffolds that
regenerate damaged tissue. A tricalcium phosphate-chitosan-fucoidan biocomposite
scaffold (TCP-Ch-Fu) using cost-effective freeze-drying technique is described for
potential bone regeneration. Methods: The functional groups, elemental
composition, phase purity, surface morphology, porosity, and in vitro human-derived
mesenchymal stromal cell attachment were examined using Fourier transform
infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDS), X-ray
diffraction (XRD), Brunauer–Emmett–Teller (BET), field emission scanning electron
microscopy (FESEM), and atomic force microscopy (AFM) respectively. Results:
The FTIR, EDS, and XRD peaks confirmed the phase purity and specific functional
groups of TCP-Ch-Fu. FESEM revealed that the cross-section pattern of the scaffold
surface was honey-comb-like structure with pore size of 26–40 µm, and surface area
of 82.65 m²/g. BET and AFM confirmed the pore pattern and surface roughness of
the material. Furthermore, FESEM also established the cytocompatibility of the
material under cell culture conditions. Conclusion: Altogether, the characterization
and cytocompatibility studies revealed that this novel scaffold may be suitable for
bone tissue engineering applications.