Table S1. Isolation source of the 40 Roseobacter strains (data primarily from Brinkhoff et al., 2008)*. Strain Phaeobacter gallaeciensis BS107 Phaeobacter gallaeciensis 2.10 Ruegeria sp. R11 Phaeobacter sp. Y4I Roseobacter sp. SK209-2-6 Roseobacter sp. MED193 Ruegeria sp. TrichCH4B Ruegeria sp. TM1040 Ruegeria pomeroyi DSS-3 Ruegeria lacuscaerulensis ITI-1157 Ruegeria sp. TW15 Rhodobacterales bacterium KLH11 Roseobacter litoralis Och149 Roseobacter denitrificans OCh114 Oceanibulbus indolifex HEL45 Roseobacter sp. GAI101 Sulfitobacter sp. NAS-14.1 Sulfitobacter sp. EE36 Roseobacter sp. R2A57 Rhodobacterales bacterium HTCC2083 Roseovarius sp. TM1035 Roseovarius sp. 217 Roseobacter sp. AzwK-3b Roseovarius nubinhibens ISM Pelagibaca bermudensis HTCC2601 Citreicella sp. SE45 Sagittula stellata E-37 Oceanicola batsensis HTCC2597 Isolation source Seawater from larval cultures of the scallop Pecten maximus Surface of the marine algae Ulva lactuca, Sydney, Australia Surface of red macroalga Delisea pulchra, Bare Island, Sydney, Australia Coastal seawater by enrichment with lignocellulose from a pulp mill, Georgia, USA From the denitrification/oxygen minimum zone at 267 m, Arabian Sea Northwest Mediterranean seawater at 1 m Trichodesmium colonies, Caribbean Sea Within the phycosphere of dinoflagellate Pfiesteria piscicida Coastal seawater by enriching for growth with dimethylsulfoniopropionate (DMSP), Georgia, USA Silica-rich geothermal lake (Blue Lagoon, Iceland) Ark clam in the South Sea of Korea Sponge Mycale laxissima, Key Largo, Florida, USA Seaweed Surface of Green alga Enteromorpha linza, Australia North Sea water, 3 km off the coast of the Helgoland, Germany Coastal seawater, Georgia, USA Surface seawater enriched with dimethylsulfoniopropionate (DMSP), North Atlantic Ocean (33°3′N, 74°3′W) Coastal seawater by enrichment with lignocellulose from a pulp mill, Georgia, USA Seawater collected 8 km west of the mouth of Yaquina Bay, Oregon, USA Seawater at 10 m from the coastal Pacific Ocean, Newport, Oregon, USA Dinoflagellate culture Pfiesteria piscicida CCMP1830 Coastal seawater, Plymouth, England Estuary of Monterey Bay, Elkhorn Slough, California, USA Caribbean Sea surface water (22°4′N, 74°4′W) BATS station, Western Sargasso Sea, Atlantic Ocean at 10 m Spartina alterniflora detritus from salt marsh near Skidaway Island, Georgia, USA Coastal seawater by enrichment with lignocellulose from a pulp mill, Georgia, USA BATS station, Western Sargasso Sea, Atlantic Ocean at 10 m Thalassiobium sp. R2A62 Octadecabacter arcticus 238 Octadecabacter antarcticus 307 Loktanella sp. SE62 Loktanella vestfoldensis SKA53 Roseobacter sp. CCS2 Oceanicola granulosus HTCC2516 Maritimibacter alkaliphilus HTCC2654 Dinoroseobacter shibae DFL12 Jannaschia sp. CCS1 Rhodobacterales bacterium HTCC2150 Rhodobacterales bacterium HTCC2255 Subsurface water 8 km west of the mouth of Yaquina Bay, Oregon, USA (44°39′N, 124°11′W) Polar sea ice and water, 350 km offshore of Deadhorse, Alaska (73°01′N, 148°31′W) Antarctic sea ice and water, McMurdo Sound, Antarctica (77°53′S, 166°35′E) Decaying Spartina along the coast of Sapelo Island, Georgia, USA Surface seawater from the North Atlantic Ocean at 2–5 m Near Bodega Head County Park, California, USA BATS station, Western Sargasso Sea, Atlantic Ocean at 10 m BATS station, Western Sargasso Sea, Atlantic Ocean at 10 m Dinophyta culture Prorocentrum lima, Helgoland, Germany Near Bodega Head County Park, California, USA Seawater at 10 m from Coastal Pacific Ocean, Newport, Oregon, USA Seawater at 10 m from Coastal Pacific Ocean, Newport, Oregon, USA *Brinkhoff, T., H.-A. Giebel, and M. Simon. 2008. Diversity, ecology, and genomics of the Roseobacter clade: a short overview. Arch. Microbiol. 189:531-539.