Raw experimental data for transformation experiments. For all data sets the
rows with grey background indicate data captured in Figure 3 of the manuscript.
Method A.
The following data were gathered using transformation method A detailed in the
manuscript. Control experiments omitted plasmid (column 2), the pulse of electric
current (column 5) or antibiotic selection (column 3).
Date set 1.
1
Strain
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
R. denitrificans
R. denitrificans
R. denitrificans
R. denitrificans
R. denitrificans
R. denitrificans
R. denitrificans
R. denitrificans
R. denitrificans
O. indolifex
O. indolifex
O. indolifex
O. indolifex
O. indolifex
O. indolifex
O. indolifex
O. indolifex
O. indolifex
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
2
Plasmid
mass (ng)
50
250
750
1000
500
1000
0
0
0
50
250
750
1000
500
1000
0
0
0
50
250
750
1000
500
1000
0
0
0
50
250
750
1000
500
1000
0
0
0
3
Chloramphenicol
(μg / mL)
7, 17
7, 17
7, 17
7, 17
0
0
0
0
7, 17
10, 17
10, 17
10, 17
10, 17
0
0
0
0
10, 17
10, 17
10, 17
10, 17
10, 17
0
0
0
0
10, 17
34
34
34
34
0
0
0
0
34
4
Colony
count
0, 0
0, 0
0, 0
0, 0
250
200
200
150
0, 0
0, 0
0, 0
0, 0
0, 0
50
20
20
20
0, 0
0, 0
0, 0
0, 0
0, 0
Lawn
Lawn
Lawn
Lawn
0, 0
0
0
0
20
150
500
lawn
500
0
5
Voltage
(kV)
2.5
2.5
2.5
2.5
0.0
0.0
0.0
2.5
0.0
2.5
2.5
2.5
2.5
0.0
0.0
0.0
2.5
0.0
2.5
2.5
2.5
2.5
0.0
0.0
0.0
2.5
0.0
2.5
2.5
2.5
2.5
0.0
0.0
0.0
2.5
0.0
6
Plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
No plasmid
No plasmid
No plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
No plasmid
No plasmid
No plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
No plasmid
No plasmid
No plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
No plasmid
No plasmid
No plasmid
1
Method A.
The following data were gathered using transformation method A detailed in the
manuscript. Control experiments omitted antibiotic selection (column 3).
Date set 2.
1
Strain
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
2
Plasmid
mass (ng)
250
250
250
250
1000
1000
1000
1000
250
250
250
250
1000
1000
1000
1000
3
Chloramphenicol
(μg / mL)
17
17
17
17
17
17
17
17
0
0
0
0
0
0
0
0
4
Colony
count
0
0
0
0
0
0
0
0
lawn
lawn
lawn
lawn
lawn
lawn
lawn
lawn
5
Voltage
(kV)
0.5
1.0
1.5
3.0
0.5
1.0
1.5
3.0
0.5
1.0
1.5
3.0
0.5
1.0
1.5
3.0
6
Plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
2
Method B.
The following data were gathered using transformation method B detailed in the
manuscript. Control experiments omitted plasmid (column 2) or antibiotic selection
(column 4).
1
Strain
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
2
Plasmid
mass (ng)
50
50
50
50
50
50
50
50
0
50
50
50
50
50
50
50
50
0
3
Ampicilin
(μg / mL)
100
100
100
100
0
0
0
0
0
100
100
100
100
0
0
0
0
0
4
Colony
count
0
0
0
0
lawn
lawn
lawn
lawn
lawn
20
100
140
200
lawn
lawn
lawn
lawn
lawn
5
Voltage
(kV)
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
6
Plasmid
pAO815
pHD1313
pRPGFPSIR2rp3
pUBeK
pAO815
pHD1313
pRPGFPSIR2rp3
pUBeK
No plasmid
pAO815
pHD1313
pRPGFPSIR2rp3
pUBeK
pAO815
pHD1313
pRPGFPSIR2rp3
pUBeK
No plasmid
3
Method C.
The following data were gathered using transformation method C detailed in the
manuscript. Control experiments omitted the pulse of electric current (column 5) or
antibiotic selection (column 3).
1
Strain
1
2
3
4
5
6
7
8
9
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
2
Plasmid
mass (ng)
500
500
500
500
500
500
500
500
500
3
Ampicilin
(μg / mL)
100
100
100
100
0
0
0
0
0
4
Colony
count
0
0
0
0
180
190
150
140
170
5
Voltage
(kV)
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
2.5
6
Plasmid
pAO815
pHD1313
pRPGFPSIR2rp3
pUBeK
pAO815
pHD1313
pRPGFPSIR2rp3
pUBeK
No plasmid
4
Heat Shock Method.
The following data were gathered using heat shock for transformation as detailed
in the manuscript. Control experiments omitted plasmid (column 2) or antibiotic
selection (column 3).
1
Strain
1
2
3
4
5
6
7
8
9
10
11
12
13
14
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
D. shibae
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
E. coli
2
Plasmid
mass (ng)
100
250
500
100
500
0
0
100
250
500
100
500
0
0
3
Chloramphenicol
(μg / mL)
17
17
17
0
0
0
17
17
17
17
0
0
0
17
4
Colony
count
0
0
0
lawn
lawn
lawn
0
200
lawn
lawn
lawn
lawn
lawn
0
5
Plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
No plasmid
No plasmid
pSB3C5
pSB3C5
pSB3C5
pSB3C5
pSB3C5
No plasmid
No plasmid
5