Supplementary data
Table S1 The property of sea water at sampling site
Cu(II)
DO
(mg/L)
(mg/L)
6.85
7.12
pH
ORP
Temperature
Conductivity
Salinity
Total nitrogen
(mV)
(℃)
(us/cm)
(‰)
(mg/L)
-60
18.1
18
31.2
1.405
Sea
7.80
water
Table S2 The sediment characteristics of sampling site
Cu(II)
Total phosphorus
Total nitrogen
Organic nitrogen
Organic
Organic
(mg/kg)
(mg/kg)
(mg/kg)
(N%)
carbon
matter
(C%)
(%)
1.02
5.10
Sediment
32.73
522.06
1780
0.18
Table S3 Closest phylogenetic affiliations of partial 16S rDNA sequences from the bands excised
from DGGE gel a
Band
Similarity
Phylogenetic
(%)
affiliation
Organism, Accession No.
No.
Isolation source
1-1
Thioalkalivibrio sp. ALMg14, EU709869
100
γ-Proteobacteria
Soda lake sediment
1-2
Tetrasphaera australiensis (T), AF125091
92
Actinobacteria
Activated sludge
2-1
Rubrobacter xylanophilus (T), X87135
94
Actinobacteria
Thermally polluted industrial runoff
2-2
Nitrospira enrichment culture Ns4a,
93
Nitrospira
Kamchatka hot spring
HM485590
3-1
Bilophila wadsworthia, AB117562
95
δ-Proteobacteria
Human feces
3-2
Magnetobacterium bavaricum, X71838
96
δ-Proteobacteria
Lake Chiemsee freshwater sediment
4-1
Alpha- proteobacterium HTB015, AB010864
97
α-Proteobacteria
South Japan deep marine sediment
4-2
Acidiphilium sp. DX1-13, EF556231
96
α-Proteobacteria
-b
5-1
Thiobacillus prosperus, EU653290
98
γ-Proteobacteria
Acidic, marine sediments
5-2
Desulfocapsa sulfexigens(T), Y13672
92
δ-Proteobacteria
Arcachon marine surface sediment , France
6-1
Alpha - proteobacterium PWB3, AB106120
96
α-Proteobacteria
Kamaishi Bay sea water
6-2
Magnetospira thiophila strain MMS-1,
95
α-Proteobacteria
Salt marsh in Woods Hole, Massachusetts
88
δ-Proteobacteria
Sediments of the hypersaline Retba Lake,
EU861390
7-1
Desulfohalobium retbaense DSM 5692,
CP001734
7-2
Desulfomicrobium thermophilum (T),
Senegal
89
δ-Proteobacteria
Terrestrial hot spring in Colombia
AY464939
8-1
Saccharospirillum impatiens(T), AJ315983
91
γ-Proteobacteria
Hypersaline Ekho Lake, East Antarctica
8-2
Pelobacter sp. A3b, AJ271656
96
δ-Proteobacteria
Black Sea shelf sediments
9-1
Olavius loisae endosymbiont 2, AF104473
95
α-Proteobacteria
Gutless marine oligochete Olavius loisae,
Annelida
9-2
Alkalilimnicola ehrlichii (T), AF406554
99
γ-Proteobacteria
Mono Lake, California
10-1
Dehalogenimonas lykanthroporepellens BL-
94
Chloroflexi
Chlorinated solvent-contaminated
DC-9, EU679419
groundwater near Baton Rouge, LA, USA
10-2
Defluvibacter lusatiensis (T), AJ132378
100
α-Proteobacteria
Activated sludge
11-1
Oceanibaculum indicum (T), EU656113
98
α-Proteobacteria
Deep seawater of the Indian Ocean
11-2
Uncultured Nitrospirae bacterium, EU780676
99
δ--Proteobacteria
Lake Miyun, Northern China
12-1
Novosphingobium sp. MG35, AJ746092
97
α-Proteobacteria
Haemodialysis water and fluid
12-2
Uncultured bacterium CM115, EF580967
94
Acidobacteria
Freshwater Calcareous Mats
13-1
Desulfocurvus vexinensis (T), DQ841177
86
δ--Proteobacteria
Deep subsurface aquifer
13-2
Desulfocurvus vexinensis (T), DQ841177
89
δ--Proteobacteria
Deep subsurface aquifer
14-1
Tepidamorphus gemmatus, GU187912
97
α-Proteobacteria
Hot spring in São Miguel, Azores
14-2
Bacteriovorax sp. DB1, EF092438
91
δ--Proteobacteria
Seawater shrimp ponds, adjacent coastal
waters
15-1
Desulfocurvus vexinensis (T), DQ841177
89
δ--Proteobacteria
Deep subsurface aquifer
15-2
Sulfate-reducing bacterium BKA11,
87
δ--Proteobacteria
Rice field soil
AJ012597
16-1
Dehalococcoides sp. BHI80-52, AJ431247
91
Chloroflexi
East Pacific Rise
16-2
Dehalococcoides sp. BHI80-15, AJ431246
90
Chloroflexi
East Pacific Rise
17-1
Uncultured delta proteobacterium, GQ850566
99
δ--Proteobacteria
Northern Bering Sea
17-2
Sulfate-reducing bacterium EZ-2C2,
90
δ--Proteobacteria
Rice field soil
AJ012598
18-1
Hirschia baltica, X52909
94
α-Proteobacteria
Phycosphere of the toxin-producing
dinoflagellate Prorocentrum lima
18-2
Amaricoccus veronensis (T), U88043
97
α-Proteobacteria
Activated sludge biomass
19-1
Clostridium bifermentans, DQ680018
98
Firmicutes
Sugarcane crop soil
19-2
Fodinicurvata fenggangensis (T), FJ357427
96
α-Proteobacteria
Salt mine in Yunnan, Southwest China
20-1
Amaricoccus veronensis (T), U88043
97
α-Proteobacteria
Activated sludge biomass
20-2
Albidovulum sp. S1K1, FJ222605
97
α-Proteobacteria
Seafloor hydrothermal chimney
21-1
Tepidamorphus gemmatus CB-27, GU187912
97
α-Proteobacteria
Hot spring in São Miguel, Azores
21-2
Rhodococcus rhodochrous (T) DSM 43241,
95
Actinobacteria
-b
99
δ--Proteobacteria
Pearl River Estuary sediments, Guangdong,
X79288
22-1
Uncultured bacterium TopBa68, FJ748800
China
22-2
Uncultured bacterium ELSC-TVG13-B68,
99
Planctomycetes
GU220761
23-1
Rhodovibrio sodomensis strain SG3105,
Low temperature hydrothermal precipitates,
East Lau Spreading Center
98
α-Proteobacteria
Mediterranean salterns
98
α-Proteobacteria
Mediterranean salterns
98
α-Proteobacteria
Mediterranean salterns
AJ318524
23-2
Rhodovibrio sodomensis strain SG3105,
AJ318524
24-1
Rhodovibrio sodomensis strain SG3105,
AJ318524
24-2
Uncultured bacterium MSB-1C5, EF125397
92
δ--Proteobacteria
Naked tidal flat sediment near mangrove
25-1
Uncultured bacterium P9X2b8D07,
99
δ--Proteobacteria
Seafloor lavas from the Loi'hi Seamount
EU491240
Pisces Peak X2
25-2
Anaerostipes sp. AIP 183.04, AY833660
91
Firmicutes
Human blood culture
26-1
Schlegelella aquatica (T)wcf1, DQ417336
91
β-Proteobacteria
Hot spring
26-2
Rhodobacter sp. 2002-65602, AY244771
96
α-Proteobacteria
Hlood culture
27-1
Microbulbifer sp. GB02-4, GQ118701
96
γ-Proteobacteria
Guaymas Basin deep-sea hydrothermal
plume
27-2
a
Flexithrix dorotheae IFO 15974, AB078038
95
Bacteroidetes
Brown sand
Two sequences of each excised band were compared to nucleotide sequences in GenBank. The closest
phylogenetic affiliation for the sequence is showed by the strain name, GenBank accession number,
similarity, phylum or class, and the isolating source. Refer to Fig.2 for band positions.
b
the isolating source not known.