Harry Bartlett, David Brazel, Josie Thiele
Results
Figure 1: 6% polyacrylamide gel (stained with SYBER-Gold) showing the samples PCR products of
the nuclear DNA of the victim and suspected canines saliva and hair run with the PEZ 2 primer and
PEZ 15 primer. Unfortunately, the suspected student’s canine saliva and hair samples did not amplify
properly in the PCR reaction. The examined bands are those that fall within the range of the PEZ 2
and PEZ 15 primer (PEZ 2 is 109-141 bp and PEZ 15 is 183-249 bp).
Figure 2: 6% polyacrylamide gel (stained with SYBR-Gold) of the victim, suspected student and Hedd
canine PCR products with PEZ -15 and VWF.X primers. The numbers on the ladder refer to the base
pair length of the aligned band and the numbers in the wells are the distance traveled in cm for the
bands within the primers range. (PEZ 15 is 183-249 bp and VWF.X is 151.187 bp).
Figure 3: 6 % polyacrylamide gel (stained with SYBR-Gold) for the PCR products of the victim,
suspected student and Hedd canine mitochondrial DNA. The numbers on the ladder refer to the base
pairs of each band at the corresponding distance.
Figure 4: 1.5% Agarose gel of the PCR products of the hair and blood samples of the victim Millstone,
as well as the hair and salivia samples of the suspected canines owned by Hedd and the student. The
gels show the PCR product of the amplified sex chromosomes locus in reference to two control male
and females; setting the control base pairs for the X chromsome to 168.5 bp, and 119.7 bp for the Y
chromosomes
Table 1: The exact base pairs of the PCR product fragments collected in their respective gels for each
primer (PEZ 2, 15, and VWF.X) as well as the two other products of sex chromosomes and
mitochondrial dna. n/a denotes values that were unobtainable or indefinable from the gels of either
the Thursday or Tuesday lab.
Sex Chromosomes
(bp)
PEZ 2 (bp)
PEZ 15 (bp)
VWF.X (bp)
Mitochondrial(bp
)
Millstone Hair
168.5/107.7
150/120
n/a
140
459
Millstone
Blood
164.2/116.6
150/120
200/150
140
459
Student Hair
116.6
150/120
n/a
n/a
463
Student Blood
164.2/114.7
150/120
190
150
457
Hedd Hair
164.2
150/120
n/a
150
436
Hedd Blood
164.2/113.5
150/120
190
155
444