TABLE 5A-1
Sample Tumor Scoring Sheet
Calendar days
SECTION LESION/TUMOR
A
CHARACTERISTICS
Dry scab/crust forming
Score
0
coherent covering with skin
Acute burst releasing
1
fluid/pus OR acute split at
border
Chronically wet/weeping
2
scab/crust OR solid yellow
matter exposed
Bleeding or raw tissue
3
exposed or white basal layer
exposed
BEHAVIORAL CHANGES
No changes (i.e., normal)
0
Repeated grooming (tumor
1
may not be easily visible or
quite small)
Abnormal gait (tumor may
not be easily visible or quite
1
MOUSE
Study
Group
ID
No.
No.
Calendar days
SECTION LESION/TUMOR
A
CHARACTERISTICS
Score
MOUSE
Study
Group
ID
No.
No.
small)
Locomotion impeded (tumor
2
is pronounced)
Recurrent scratching/biting
3
of tumor
Nociception
3
(struggling/sqeaking) on
touching tumor
IF YOU HAVE SCORED ANY 3s IN SECTION A, CULL TODAY.
IF YOU HAVE SCORED ANY 2s IN SECTION A, REFER TO SECTION B, ELSE GO TO SECTION C.
SECTION SIZE/PROGRESSION OF LESION
B
(expressed in orthogonal
#
D1/D2**
diameters**)
Shrinking
0
Static
1
2–3 mm growing
2
3–5 mm growing
3
5+ mm growing
4
Calendar days
SECTION LESION/TUMOR
A
CHARACTERISTICS
Score
MOUSE
Study
Group
ID
No.
No.
SIZE/PROGRESSION OF
TUMOR
Shrinking
0
Static
1
10–12 mm growing
2
12–14 mm growing
4
14+ mm growing (mean
6
D1/D2 or D1 or either > 17
mm)
TOTAL FOR SECTION A +
SECTION B
**# D1 and D2 are orthogonal
diameters
IF THE TOTAL FOR SECTIONS A + B IS 6, CULL WITHIN 1 DAY. IF 4–5 MONITOR DAILY, CULL WITHIN 1 WEEK IF
NO IMPROVEMENT.
ELSE GO TO SECTION C
SECTION KEEP MONITORING AS
C
REQUIRED (DAILY)
Calendar days
SECTION LESION/TUMOR
A
CHARACTERISTICS
Score
MOUSE
Study
Group
ID
No.
No.
Date
Adapted with permission from a scoring sheet developed by Fraser Darling, The Institute of Cancer Research,
London, UK.
Recognition and Alleviation of Pain in Laboratory Animals
National Research Council (US) Committee on Recognition and Alleviation of Pain in Laboratory Animals
Washington (DC): National Academies Press (US); 2009.
ISBN: 978-0-309-12834-6
Copyright © 2009, National Academy of Sciences