ROLE OF EDUCATION IN IDENTIFYING ENVIRONMENTAL OPPORTUNITIES AND THREATS OF BIOTECHNOLOGY (GMO, LMO) (CASE STUDY: STUDENTS IN ENVIRONMENT) Seyedeh Maryam Mashalchi1, Ali Reza Ahmadi2* 1 Department of Environment Management and Education Planning: Environmental Educaion, Faculty of Environment and Energy, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2 Woman Research Centre, Department of Biomedical Sciences, Alzahra University, Tehran, Iran. Abstract Emergence of new sciences and their development and impact on environment cannot be denied. Biotechnology and technology of production of genetically modified products and specifically GMO and LMO is one of these sciences which have interaction and overlap with environmental issues in different parts. Such overlap and interaction have caused worry among supporters of the environment and sometimes there have been biases in this regards leading to confuse and sometimes losing opportunities for utilization of this science. The reason for worries is somehow lack of appropriate education in this regards to the students and supporters of the environment. Current research was conducted as field study using questionnaire and investigated role of education in identifying environmental opportunities and threats of biotechnology among students of biotechnology in some universities of Tehran. Statistical population was considered as 1000 and sample size was considered as 278. Data obtained from the sample were classified according to the age and educational level. Also, 70 people were considered as case group which included students of bioscience in Tehran universities. Following collection and analysis of data, results of pair-wise t test showed there is significant relationship between education and knowledge of people. Also, results showed there is significant relationship between age and education and educational level and education. Finally it was specified that with education, knowledge of people toward identifying environmental opportunities and threats of biotechnology is increased. In addition, it is increased also with increasing age and educational level and it is in line with case group. Findings show face-to-face education has higher impact on increasing knowledge compared to online education. Keywords: environmental education, education, environmental opportunities of biotechnology (GMO and LMO), environmental of threats of biotechnology (GMO and LMO), knowledge. Introduction The term biotechnology was firstly applied by Karl Earky in 1919 in the meaning of application of bioscience and its interaction in man-made technologies. Overall, any smart action of human in creation, improvement and supply of various products using creatures, especially through manipulation at molecular level is considered in the scope of technology of current century (Tabandeh, 2006). Considering rapid progress of biotechnology science, some worries have been developed because of adverse impacts of products of this science, because some institutes and supporters of environment oppose to this science because of its harm to the environment and they view some benefits and characteristics of biotechnology as myth and believe that reality is something else (Claire, 2011; Antoniu, 2012). Given rapid growth of the world population and increased demand for food and health, undoubtedly in future years it would lead to social, political, and economic threats and environmental sustainability would be threatened (Salehi Jouzani et al., 2010). Some scholars and academic communities argue that GMO or transgenic 0or manipulated crops can save many people of the world from hunger caused by famine and drought and solve many problems resulting from population growth. They consider biotechnology as powerful tool for sustainable development which can help developing and developed countries. Considering history of biotechnology evolution it is found that the human used this science since 600 years before Christ birth in Egypt and utilized microbial process for protection of his interests in the era of biotechnology era and he used its products unconsciously and this wave of development cannot be easily stopped (Balali et al., 2009). Significance of this study is due to special attention to biotechnology in Iran and many other countries, as Environmental Protection Agency believes protection of biodiversity elements is necessary for achievement of sustainable development and such protection depends on preservation of gene for future generations and transfer of maximum biodiversity to them (Mirderikvand et al., 2010). Genetics engineering is mentions such cases as increase in grain production, reforming food quality, use of transgenic plants (Salehi Jouzani et al., 2010), and biological fertilizers and energy and environment management using GM crops (Brown, 2007), as benefits of biotechnology. Importance and high ability of genetic engineering and biotechnology is elimination of limitations in developing new features and quality and quantity of food, pharmaceutical, agricultural, medical products have caused that this technology quickly gains special status in production cycle of many countries including USA, Canada, India and Iran. current research attempts to investigate role of education in identifying environmental opportunities and threats in groups which are trained, and the results are compared with results of trained group so that significance and impact of education on decision making by these people in this field and need for such education is specified. Theoretical Foundation Education in Moeen Dictionary means learning and teaching (Moeen Dictionary, 2009), and in more academic terms, according to Bloom it means realization of learning in knowledge process, insight, understanding, perception, application, skill, analysis and purposeful participation so that education process is designed accordingly (Lahijanian, 2011). Education and informing is important because it refers to health of the society and human beings. But people should not be intimidated during education. Giving wrong and undue and excessive information creates feat in people. Informing should be in the direction this people are informed and aware of advantages and disadvantages of cases and education (Nohadani Marzi, 2011). Environment is made of two terms: environment and living. Environment means surrounding and it is a segment enclosing the space (Moeen Dictionairy, 2009) and living means life. Environment means a collection of external physical factors and living creatures which interact each other. Environmental education seeks for nurturing citizens which are aware of biophysical environment and its respective issues and can help in solving them and are eager to work for implementing the solutions (Azimi et al., 2012). Overall, protection of environment is necessary for achieving goals of sustainable development. Various studies have been done regarding protection of environment and paying attention to education and educational promotion for encouraging people to protect the environment at any form is very important (Rafiee and Amirnejad, 2009). Environmental education structure is composed of formal education, informal education and implicit education. On the other hand, biotechnology includes the technology of using living creatures with peaceful and humanitarian purposes for human welfare and environmental protection. Agricultural biotechnology is the science which provides purposeful production of plants and animals with optimal attributes for the human through techniques of Recombinant DNA and special biotechnological products. This science has considerably increased agricultural products in recent years. Application of biotechnology in the industry, which leads to production of various products at low cost and less energy, less waste and less harmful effects on the environment, have caused that this technology is famous as the cleanest and most profitable sectors of the industry. Production of products which was not possible already or their production was very difficult now is possible using biotechnology. Biotechnology education means explanation of concepts about biotechnology including history, general techniques in biotechnology, definition of terms of environmental issues, global figures for progress of this science, applications, and its environmental advantages and disadvantages for people under education which is done in two ways: face-to-face education or holding seminars, general workshops, lectures and online education through email. Educational materials are prepared from academic sources and the presentation is done as Power Point. In face-to-face education, an environmental specialist and a specialist in biotechnology offer the education. GMO and LMO are discussed in this research because they are main biotechnological products and they are actually genetically modified products. Review of Literature Some domestic and foreign research works in this regards include as follows: Gavrilescu in his paper entitled Environmental Biotechnology, Achievements, Opportunities and Challenges mentioned four keys of environmental biotechnology entry to environment in terms of pollution as pollution control, waste management, pollution prevention and removal of production pollution and interference in the industrial process (Gavrilescu, 2010). Hoseini viewed role of education at different levels of society as important and priority and these educations may have positive impacts on environmental behaviors of citizens at society level, and considering importance of citizen role, it can be considered as a social subject in line with improvement of worrying situation of environment. Shirani stated that urban environmental educations influence behavior and value making of students. Factors such as educational level and income of individuals influence education. The study by Rafiee on 400 people of Mazandaran province using contingent valuation method and function Logit Regression showed although functions like educational level and income level influence this increase, paying attention to education and educational promotion for encouraging people to protect the environment is very important (Rafiee and Amirnejad, 2009). In the study on six groups from three cities, gender and education and age of people was consider and it was found most of subjects do not pay attention to the production way, though they care for the price and features of food products. Also, it was stated there is close relationship between low awareness level and understanding materials related to GM foods. In this research, variables of gender, age, and education were considered in investigation of attention of individuals to biotechnology (Theis et al., 2002; Claire, 2011). In the paper published by Dawson, findings showed perception of students was increased following completion of a biotechnology education course, but their attitude toward human use of genetic technology stayed intact except their opinions. Research Model and Hypotheses Figure 1 indicates research conceptual model. Independent variable Biotechnology educational Plan Dependent variable Knowledge Fig 1. Research conceptual model Considering conceptual model, research hypotheses are as follows: 1. H1a: Implementation of education program promotes knowledge of students toward environmental opportunities and threats of biotechnology (GMO, LMO) 2. H2a: Age of trained students influences improvement of students toward environmental opportunities and threats of biotechnology (GMO, LMO) 3. H3a: Educational level of trained students influences improvement of students toward environmental opportunities and threats of biotechnology (GMO, LMO). 4. H40: There is no mean difference between education group of posttest and control group in terms of knowledge toward environmental opportunities and threats of biotechnology (GMO, LMO). 5. H5a: Type of education (face-to-face and online) in trained students influences improvement of students toward environmental opportunities and threats of biotechnology (GMO, LMO). Nature, Method and Variables of Research Current research is fundamental research and semi-experimental in terms of purpose. Since provided educations would increase knowledge of subjects in case of effectiveness. It is an applied research due to nature of its purpose and investigation of education effectiveness role and use of education as a method for solving environmental and biotechnological issues. In this research, for investigation of education role and its impact on knowledge and attitude of individuals and thus its impact on ability to identify and diagnose environmental opportunities and threats of biotechnology, knowledge and attitude of individuals before and after education would be evaluated. To this end, firstly pretest is done and then education is provided and posttest is done. Also, for judgment about posttest results and education results, the obtained results are compared to control group. In the current research, considering that relationship between education and knowledge and attitude is identified and described, it is descriptive in terms of data collection method. Given that effect of a phenomenon is investigated in two statuses, before and after education, effect of education is studied through investigation of difference before and after education. Due to presence of control group, it is an action research. In this research, dependent variable of knowledge is measured in the research model. Biotechnological plan is considered as independent variable. Data Collection Methods Library studies and referring to documents and field study were used for data collection in this research. The questionnaire was also used. The questionnaire was prepared considering educational concepts and was written with approval of advisor. Firstly the questionnaire was prepared and secondary questionnaire was written and distributed among statistical sample following approval of advisor and other experts. Pretest and posttest questionnaires were used in this research. Pretest questionnaire items were given in individual and general items and specialized items. Posttest questionnaire was given in two parts including individual items and specialized items with 10 knowledge items and 29 attitude items with five-point Likert scale. Following designing initial questionnaire, validity and reliability of questionnaire was determined as follows: content reliability of this questionnaire was confirmed by informed and expert people, and it has acceptable reliability. Cronbach's alpha method was used for determining reliability. Cronbach's alpha was measured as 0.890 for total items and values were obtained for each of criteria. These values indicate that the questionnaire has acceptable reliability or in other words, it is reliable. Statistical Population, Sample and Sampling Method Statistical population in this research includes all students of environment and bioscience (n = 1000). In order to provide data needed in this research easily, nonprobability sampling simple method was used. Simple sampling method is a kind of sapling which uses individuals and units in the sample under study which are accessible for ease of work. In this research, Morgan table was used for estimation of sample size. Considering Morgan table for population of 1000, selective sample size was 278. Data Analysis Method In this research, descriptive statistics and inferential statistics were used for analysis of data obtained from samples. In fact, firstly research variables were tested using descriptive statistical methods. In this research, descriptive statistics are used for determining mean and SD of indexes. Statistical methods for data analysis are described in the following. Research Demographics Investigation of demographic characteristics of sample showed 119 (43%) participants received face-to-face education and 159 (57%) received online education. Investigation of the sample demographics in terms of educational level revealed that 120 participants (43%) had Ba degree, 124 ones (45%) had MA degree, and 35 ones (12%) had PhD degree. Investigation of demographic features in terms of age showed 29 percent of participants had below 25 years and 11 percent were above 45 years old, 35 percent were between 25 – 35 years, and 25 percent were between 35 – 45 years. Descriptive Statistics of Research Hypotheses In this section, descriptive statistics techniques such as frequency tables and statistical diagrams are used for data analysis and investigation of statistical sample distribution in terms of research hypotheses. Following diagrams were designed according to answers of respondents in five-point Likert scale from totally disagrees to totally agree. It should be noted that type of answer of respondents was as follows. Variable of students’ knowledge towards environmental opportunities and threats of biotechnology (GMO, LMO) was made of sum of means of Items 1- 10. Variable of students’ attitude toward environmental opportunities and threats of biotechnology (GMO, LMO) was considered from sum of Items 11 – 39. Report of this mean is evident in following diagrams. Since mean of answers for two variables is obtained as decimal, it is observed that scale level is not exactly between 1 to 4 points of Likert in the following diagrams. SPSS software introduces the points where mean of values are more accumulated and shows percentage of respondents. 70.00% 50.00% 40.00% 30.00% 20.00% 10.00% 0.00% Percent of answers to items of questionnaire 60.00% 61.00% 24.00% 8.00% 4% 2.00% 3.3 3.6 3.9 4.2 4.5 Frequency of options based on Likert scale with change from SPSS Fig 2. Knowledge level of students toward environmental opportunities and threats of biotechnology 40.00% 35.00% 30.00% 25.00% 20.00% 15.00% 10.00% 5.00% 0.00% and threats of biotechnology (GMO, LMO) 45.00% 48.00% Percent of answers to items of questionnaire regarding environmental opportunities 50.00% 26.00% 14% 10.00% 2.00% 4.6 4.7 4.8 4.9 5 Frequency of options based on Likert scale with change from SPSS Fig 3. Knowledge level of students toward environmental opportunities and threats of biotechnology after education Pairwise Mean Test (Pairwise) Pairwise comparison test or dependent samples is based on t distribution of a nonparametric test in which mean of the population in two situations is investigated. In order to achieve research goal, five hypotheses were considered which are provided as follows. Hypothesis 1 In this test, if p=value < α = 0.05, equality of means is rejected and the hypothesis is rejected and assumption of presence of difference is accepted. Table 1: pairwise t test for investigating equality of mean of students’ knowledge toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Title Group No Mean students’ knowledge toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Before education 278 3.63 After education 278 4.06 Degree of Mean Freedom Increase 267 0.43 P-Value 0.00 Considering Table 1 and results of test, it is observed since p-value = 0.00, thus assumption of equality of means in two stages is rejected. That is, collected data before and after education have different means and mean difference is 0.43. It is a positive value which denotes data have mean increase in the stage after education. That is, mean environmental knowledge of students towards environmental opportunities and threats of biotechnology (GMO, LMO) after education is better and higher than mean of students before education. Hypothesis 2 In this test, if p=value < α = 0.05, equality of means is rejected and the hypothesis is rejected and assumption of presence of difference is accepted. Table 2: pairwise t test for investigating equality of mean of trained students’ age in knowledge improvement toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Title Group trained Before students’ age in education knowledge improvement toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education After education Below 25 No Mean 278 3.61 Degree of Mean Freedom Increase 276 Below 25 25 35 – 3.63 0.45 25 – 35 35 45 – 3.66 0.44 35-45 Above 45 Below 278 25 25 – 35 35 – 45 Above 45 3.61 0.38 Above 45 4.06 0.46 P-Value 0.00 4.07 4.04 4.07 Considering Table 2 and results of test, it is observed since p-value = 0.00, thus assumption of equality of means in two stages is rejected. That is, collected data before and after education have different means. It is a positive value which denotes data have mean increase in the stage after education. That is, with increasing age of trained students, trend of students’ knowledge improvement towards environmental opportunities and threats of biotechnology (GMO, LMO) was better after education compared to before education. It firstly showed reduction and then it was increased. Hypothesis 3 In this test, if p=value < α = 0.05, equality of means is rejected and the hypothesis is rejected and assumption of presence of difference is accepted. Table 3: pairwise t test for investigating equality of mean of trained students’ educational level in knowledge improvement toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Title Group trained students’ educational level in knowledge improvement toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Before education After education educational level BA No Mean 278 3.64 Degree of Mean Freedom Increase BA BA MA 3.64 139 0.43 PhD 3.58 MA MA 168 0.41 PhD PhD 41 0.47 BA 278 P-Value 0.00 0.00 4.07 MA 4.05 PhD 4.06 0.00 Considering Table 3 and results of test, it is observed since p-value = 0.00, thus assumption of equality of means in two stages is rejected. That is, collected data before and after education have different means. It is a positive value which denotes data have mean increase in the stage after education. That is, mean of educational level in trained students was better after education in students’ knowledge improvement towards environmental opportunities and threats of biotechnology (GMO, LMO). Hypothesis 4 In this test, if p=value < α = 0.05, equality of means is rejected and the hypothesis is rejected and assumption of presence of difference is accepted. Table 4: pairwise t test for investigating equality of mean of educational group in posttest and control group in terms of knowledge and attitude toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Title Group No Mean Degree of Mean Freedom Increase P-Value mean of educational group in posttest and control group in terms of knowledge and attitude toward environmental opportunities and threats of biotechnology (GMO, LMO) before and after education Posttest group data Control group 278 3.74 278 4.27 267 0.52 0.00 Considering above table and results of test, it is observed since p-value = 0.00, thus assumption of equality of means in two stages is rejected. That is, collected data before and after education have different means. It is a positive value which denotes data have mean increase in the stage after education. That is, data of control group is higher in terms of mean test compared to posttest group. Thus, mean of educational level in trained students was less than mean of control group students. Hypothesis 5 In this test, if p=value < α = 0.05, equality of means is rejected and the hypothesis is rejected and assumption of presence of difference is accepted. Table 5: pairwise t test for investigating equality of mean of educational group in posttest and pretest among face-to-face students and online students in terms of knowledge and attitude toward environmental opportunities and threats of biotechnology (GMO, LMO) Title Education Type Type of Face-toeducation of face trained students in improvement of knowledge toward environmental Online opportunities and threats of biotechnology (GMO, LMO) before and after education Group No Mean Before education 278 3.25 After education Before education After education Degree of Mean freedom Increase 276 0.49 P-Value 0.00 3.75 278 3.29 0.46 3.75 Considering Table 5 and results of test, it is observed since p-value = 0.00, thus assumption of equality of means in two stages is rejected. That is, collected data before and after education have different means. It is a positive value which denotes data in online group have lower mean increase compared to face-to-face group. Conclusion Considering statistical results and investigation of research hypotheses and research data analysis, in Hypothesis 1 it is assumed that with provision of education, knowledge level of students toward environmental opportunities and threats of biotechnology is increased. It is observed that mean scores of subjects in posttest is increased compared to mean scores in pretest which suggests positive impact of education on knowledge level of students. Thus, education can be considered as a factor in increasing knowledge level related to environmental opportunities and threats of biotechnology in students. Overall, it can be concluded that education is effective on increasing ability to identify environmental opportunities and threats of biotechnology among students and learners, since adopting decision and identifying opportunities and threats require awareness. If the people are aware and informed, they would be able to make decision. Investigation of results for data analysis and result of pairwise comparison tests in second hypotheses showed that age is also effective in increasing knowledge level resulting from education and knowledge level of subjects was increased after education. In fact, with increasing age, the sensitivity toward environmental issues and impact of biotechnology on environment is also increased. Overall it is concluded educational level influences increasing knowledge resulting from education in students toward impact of biotechnology on environment, and this impact and diagnosis is increased by increasing educational level from BA to MA and PhD. Considering results of investigation of second and third hypotheses it is found that increasing age and educational level increases sensitivity toward environmental issues and its challenges with new sciences. Investigation of results related to mean of control group and posttest from case group indicates that mean of control group has higher positive mean compared to mean of posttest group. However, considering mean of posttest group has also positive growth and it is in line with mean of control group, it can be conclude direction of education and educational content is appropriate and provided education was suitable and effective. Investigation of third hypotheses and results for comparison of face-to-face education and online education show that face-to-face education is more effective and increasing knowledge level for identifying environmental opportunities and threats of biotechnology is higher. Thus, it can be concluded that face-to-face education can be more effective in increasing education impact due to attracting attention of learners and responding possible questions. References Balali, G., Taheri, R. & Mallory, G. (2009). Biotechnology and its application in modern agriculture, Journal of Agricultural Jihad Organization of Isfahan Province, Agricultural Extension Coordination Management and Implementation of Promotional Media Planning Tabandeh, F. (2006). Iran's national document of biotechnology / national biotechnology strategy planning project, Tehran: Ahar Rafiee, H. & Amirnejad, H. (2009). Role of education in increasing people's willingness to protect the environment (Case Study: Caspian Sea). Environmental Science, pp. 95-108. Salehi Jouzani, G., Tohidfar, M. Sadeghi, A. (2010). Bio-safety of GM products, Karaj: Agricultural Biotechnology Research Center. Azimi, M., Karami, A. & Gholami, M. (2012). Increasing public environmental awareness with an emphasis on environmental education, Fourth National Conference on Education Mirderikvand, M., Rahaee, M., Marvi, M. & Lotfi, A. (2010). Status of strategic technologies in Fifth Development Plan with emphasis on biotechnology, Tehran Lahijanian, A. (2011). Environmental education, Tehran, Islamic Azad University, Science and Research Branch Nohadani Marzi, M. (2011). Environmental protection and biotechnology in national and international law, MS Thesis in Environmental Law (School of Environment and Energy), Islamic Azad University, Science and Research Branch Antoniu, Michael. And Robinson, Claire. And Fagan, John (2012). GMO Myths and Truths. Published by Earthopensource.9. Brown, Sheldon. S (2007). Opportunities in Biotechnology Careers. ISBN: 0-07197605. Published by McCarow-Hill. Claire, James (2011).ISAAA, International Service for the Acquisition of AgriBiotech Applications (2011). Value Data are explicitly from seeds and licensing revenue rather than from crops themselves. http://www.isaaa.org/ Gavrilescu, Maria (2010). Environmental Biotechnology, Achievements, Opportunities and Challenges. Dynamic Biochemistry, Process biotechnology and Molecular Biology. Global Science Books. Vol.4, no.1. 1- 36. ISSN 1749-0626. Teis, Mario f. and Halverson, Lynn and O’Brein, Brian roe and Ross, nancy and vayda, Mike (2002). Focus group Reactions to Genetically Modified Food Lables.