Simon Fraser Animal Care Services:
Standard Operational Procedures
Table of Contents
Introduction ...................................................................................................................................... 1
a.) Simon Fraser University Animal Care Committee ................................................................ 2
b.) Protocol Forms and Procedures ............................................................................................. 4
c.) Organization and Personnel ................................................................................................... 5
d.) General Animal Husbandry at Simon Fraser University Animal Care Facility ..................... 8
e.) Facilities Management in Regards to Introduction of Equipment, Animal Movement within the
Facility and Waste Disposal ................................................................................................... 9
f.)
Simon Fraser University Animal Care Facility .................................................................... 12
A1. ANESTHETIC MACHINE
A1. APPENDIX: INDUCTION WITH ISOFLURANE
A2. GUINEA PIG ANAESTHESIA
A3. HAMSTER AND GERBIL ANAESTHESIA
A4. RABBIT ANAESTHESIA
A5. RAT ANAESTHESIA
A6. MOUSE ANAESTHESIA
A7. SHOREBIRD ANAESTHESIA
A8. PASSERINE ANAESTHESIA
A9. AMPHIBIAN ANAESTHESIA
A10. REPTILE ANAESTHESIA
A11. DOMESTIC BIRD ANAESTHESIA
A12. FISH ANAESTHESIA
C1.
C2.
RAT COLONIES
MOUSE COLONIES
D1.
D2.
D3.
D4.
D5.
D6.
D7.
D8.
CLINICAL EXAMINATIONS
OPHTHALMIC EXAMINATION
BLOOD COLLECTION
COLLECTION AND HANDLING OF SPECIMENS FOR BIOCHEMISTRY
URINE COLLECTION
FECAL FLOTATION EXAMINATION FOR INTERNAL PARASITES
EXAMINATION FOR EXTERNAL PARASITES
ANIMAL HEALTH SURVEILLANCE PROGRAM
E1.
E2.
EUTHANASIA
E1. APPENDIX: FISH EUTHANASIA
CHANGING THE CO2/O2 CYLINDERS
F1.
F2.
F3.
FACILITY MAINTENANCE
CLIMATE CONTROL
LIGHTS AND TIMERS (ACF and TASC2-ARC)
F4.
F5.
F6.
F6.1
F6.2
F6.3
F7.
F8.
F9.
F10.
F11.
F12.
F13.
F14.
F15.
F16.
F17.
F18.
F19.
F20.
F21.
F22.
F23.
G1.
G2.
G3.
G4.
G5.
G6.
G7.
G8.
G9.
G10.
G11.
G12.
H1.
H2.
H3.
H3.1
H4.
H5.
H6.
H7.
H8.
MONITORING TEMPERATURE, PRESSURE AND HUMIDITY
CLOTHING IN THE ANIMAL CARE FACILITY
CLOTHING IN THE TASC2- ANIMAL RESOURCE CENTRE (ARC)
ENTRY TO BARRIER
ENTRY INTO ANIMAL ROOMS
ENTRY INTO THE ARC SERVICE AND ISOLATION/QUARANTINE AREAS VIA
DIRTY RECEIVING 6200 AND CLEAN RECEIVING 6300 DELIVERY AND SERVICE
PERSONNEL ARRIVING FROM OUTSIDE THE BUILDING
LAUNDRY
RECEIVING SUPPLIES (FEED AND BEDDING)
MONITORING OF ANIMAL BEDDING
ACF WASTE AND CARCASS DISPOSAL
ACF FREEZERS
BIOLOGICAL WASTE COLLECTION AND INCINERATION
SURGERY (ROOM 120) AND PROCEDURES (ROOM 119) ROOM MAINTENANCE
BIOHAZARD ROOM – LEVEL 2
GENERAL RULES FOR WORKING WITH RADIOISOTOPES
OCCUPATIONAL HEALTH AND SAFETY PROGRAM RELATED TO HANDLING OF
LABORATORY ANIMALS
ACCIDENT REPORTING
FIRE REGULATIONS
FUMIGATING ROOMS (ACS)
CHEMICAL DISPOSAL
CHEMICAL SPILL RESPONSE
BIOHAZARD SPILL RESPONSE
ENTRY AND EXIT PROCEDURES
RECEIVING ANIMALS
IDENTIFICATION OF ANIMALS
HOUSING OF ANIMALS
GENERAL ANIMAL HANDLING
G4. APPENDIX: RESTRAINT OF THE RAT
CENSUS TAKING AND RECORDS
CLINICAL OBSERVATION OF ANIMALS WHILE ON TEST
PROCEDURES FOR DEAD MORIBUND ANIMALS
PROCEDURES FOR ESCAPED RODENTS
DISEASE OUTBREAK CONTAINMENT
RODENT HEALTH MONITORING (SENTINEL PROGRAM)
SHIPPING OF LIVE ANIMALS RODENT HEALTH MONITORING
QUARANTINE PROCEDURES FOR IMPORTATION OF BIRDS FROM OTHER
FACILITIES
DAILY ROUNDS
OPENING AND CLOSING PROCEDURES (ACF)
SMALL RODENT HUSBANDRY
RODENT HUSBANDRY-DIRTY QUARANTINE
H3.1-1: RODENT HUSBANDRY-DIRTY QUARANTINE MUREIN NOROVIRUS+
RABBIT HUSBANDRY
RABBIT NAILS AND TEETH
GUINEA PIG HUSBANDRY
HAMSTER HUSBANDRY
SMALL PASSERINE BIRD HUSBANDRY
H14.
H15.
H16.
H17.
H18.
H8. APPENDIX: SMALL PASSERINE BIRD HUSBANDRY ACF AND WORKSTUDY
INFORMATION
WATERFOWL HUSBANDRY
HUSBANDRY PROCEDURES – CAGE WASHING
AMPHIBIAN HUSBANDRY
H11. APPENDIX: LAB SPECIFIC XENOPUS FROG HUSBANDRY
FISH HUSBANDRY
H12. APPENDIX: LAB SPECIFIC FISH HUSBANDRY
SERVICING A RODENT ROOM AFFECTED WITH PINWORM INCLUDING THE
REGIME FOR REMOVAL OF DIRTY CAGING AND EQUIPMENT FROM THAT ROOM
TECHNICAL CHANGING A RODENT CAGE (STATIC CAGES)
CHANGING A RODENT CAGE (IVC) – BARRIER
CONVENTIONAL RODENT ROOM
MODIFIED BARRIER RAT ROOM TASC2-ARC
TRANSGENIC MICE HUSBANDRY
I1.
I2.
I3.
IMMUNIZATION WITH FREUND’S ADJUVANT
IMMUNIZATION WITH TITERMAX
IMMUNIZATION WITH RIBI ADJUVANT SYSTEM
H9.
H10.
H11.
H12.
H13.
M1. HOBART C44 CAGE WASHER
M2(A). AUTOCLAVES PELTON AND CRANE LABORATORY STERILIZER
M2(B). AUTOCLAVES PEL TUTTNAUER AUTOCLAVE – MODEL #3870-E
M2(C). AUTOCLAVES STERIS AUTOCLAVE –AMSOCO® CENTURY TM MEDIUM STEAM
STERILIZER
M3. BLOWDOWN OF STEAM GENERATOR
M4. ELECTRONIC SCALE – PROCEDURES FROOM (ACF ROOM 119)
M5. PHARMACAL POWER SPRAYER – MODEL #99940
M6. “BIOBUBBLE” CHANGING STATION – TRADEMARK SERIAL #764, 137
M7. CHEMICAL APPLICATION FOR FOGGING ROOM – “DYNA-FOR CYCLONE” MODEL
NO 2730
M8. IN-HOUSE VACUUM SYSTEM
M9. CRYOSTAT CRYOTOME® SME
M10. ULTRASONIC CLEANING SYSTEM (ULTRONICS ULI)
M11. TIAC CENTRIFUGE – MODEL NO 0206
M12. MOBILE POWER SPRAYER
M13. THE INOTECH DRY STERILIZER – STERI 350
M14. ERHRET BIOLOGICAL AIR SYSTEM – BIO A.S. AIR SUPPLY UNIT
M15. CASTEL BIOSIGN INCUBATOR – MODEL #61301600055; 55 (STEAM)
M16. EYEWASH BOTTLE (ACF)
M17. EQUIPMENT DECONTAMINATION – BIOQELL CLARUS C IN VHP ROOM 6400.1
N1.
N2.
N3.
N4.
NECROPSY EXAMINATION – SMALL ANIMAL (RABBITS)
NECROPSY EXAMINATION - RODENT
STORAGE AND DISPOSABLE OF FIXED TISSUE
SPECIMEN COLLECTION FOR HISTOLOGY
S1.
S2.
S3.
S4.
MINIMUM STANDARDS FOR PERFORMING ASEPTIC SURGERY
ANIMAL PREPRARATION FOR SURGERY
SURGEON PREPARATION FOR SURGERY
POST OPERATIVE CARE
S5.
S6.
S7.
S8.
S9.
S10.
OVARIECTOMY IN RODENTS
ORCHIDECTOMY IN RATS
TFM ORCHIDECTOMY IN RATS
OOCYTE HARVEST IN FROGS
RADIO TRANSMITTER IMPLANTATION IN THE COELOMIC CAVITY OF BIRDS
GUIDELINES FOR RODENT SURVIVAL SURGERY
T1.
T2.
T3.
T4.
T5.
T6.
T7.
T8.
ADMINISTRATION OF MEDICATIONS
TREATMENT OF COMMON RABBIT AILMENTS
TREATMENT OF COMMON MOUSE AILMENTS
TREATMENT OF COMMON RAT AILMENTS
TREATMENT OF COMMON HAMSTER AILMENTS
TREATMENT OF COMMON GUINEA PIG AILMENTS
EMERGENCY CARE PROCEDURES
HANDLING MEDICATIONS AND CONTROLLED DRUGS
W1.
W2.
W3.
W4.
W5.
W6.
W7.
W8.
AQUARIUM FOOM DESCRIPTIONS
AQUARIUM WATER QUALITY
FISH HEALTH
AQUATIC DISEASE CONTROL
ANIMAL CARE REQUIREMENTS
SAFETY AND SECURITY PROCEDURES OF THE AQUATIC FACILITY
POLICIES FOR THE AQUATIC FACILITY
AQUATIC FACILITIES EQUIPMENT AND MAINTENANCE