-80 Freezer
Ashing Furnace

Frostbite

Laceration

Fire

Frost burns to skin and eyes

Fire




Hot surface or materials

Explosion by overheating




Burns

Injuries
Severity Likelihood Risk
Level
3 3
Measures to reduce the risks posed by hazards
( Lab coat and gloves must be worn at all times when working in lab )
9

Dry cold protection gloves must be worn

Do not put glassware in the freezer

Do not store flammable liquids in the
 freezer
The freezer is locked and is accessible only to authorized lab staff.
2 2 4



3 3 9

Only to be operated by trained lab staff.

Thermal gloves must be worn

Tongs must be used when removing
 materials from the furnace

Do not exceed the max heating limit
No flammable materials are allowed for ashing

Atomic Absorption
Spectrophotometer

Hazardous radiation from flame/hollow cathode/D2 lamp

Acetylene explode

Heat from burner head

Eye damage

Burns

Gas leak
3
Atomic Force
Microscope
Autoclave


3

Heat/Steam

Contamination with bio-hazardous substances

Explosion

Burns

Infections

Eye/body injuries
3
3
3
3
9

Students must be guided by trained lab staff

Never directly view the flame/hollow cathode/D2 lamp.

Acetylene gas pressure should not exceed 15psi

Perform periodic gas leak test

If removing burner head ,allow sufficient time for the burner head to cool

Preventive maintenance should be done
9 yearly


9

Only to be operated by trained lab staff

Thermal gloves must be worn

The chamber lid must be properly closed
 before operation.
Never use autoclave for explosive/combustible/oxidizing

/flammable substances.
Daily maintenance is essential.

Preventive maintenance should be done annually

Biosafety Cabinet

Exposure to UV light

Exposure to
Microorganisms

Burns

Infections
Drilling Machine

Flying Chips

Eye/body injuries
3
Bunsen burner

Heat/Flame

Gas leak

Burns

Fire
Centrifuge

Samples leaks

Samples imbalance


Aerosols, chemicals and microorganisms contamination

Machine malfunction

3
3
3
3
3
3
2
3
3
9

Users must be trained and strictly follow safety laboratory practices

Do not stand near when the UV is on.

Turn off UV before use

Do not use flammable/combustible/ explosive substances in the unit.

Preventive maintenance should be done annually
9

Wear thermal gloves if necessary

If gas is smelt, turn off tap and report
 immediately
Do not have or use flammable/volatile/explosive substances near the flame.
6

Users must be trained

Never fill the centrifuge tubes above the
 max limit
Never exceed the max speed

Centrifuge tubes must be balanced
9


9

Always secure the workpiece on the vice

Wear goggles

Student must be guided by trained lab staff

Electrophoresis System

Electric shock

Short-circuiting

Toxic chemicals used
Fractional
Distillation/Reflux
System/Rotary
Evaporator
Fume Hood

Release of evaporating content

Flask falling off or cracking of glassware

Injuries

Protein gel is toxic

Inhalation of toxic fume.

Injuries
3
3
 flammable liquids
 hazardous fumes/substances

Fire/Explosion

Exposure to hazardous substances
3
2
3
3
6

Polyacrylamide gel can only be casted by trained lab staff or to purchase commercially.

Metal components should not be touched.

Electrodes must be connected to their respective sockets.

Student must be guided by trained lab staff
9

Student must be guided by trained lab staff

Do not operate near flammable vapour or exposed flame.

To be done in fume hood with the front door down

Heating mantle is used instead of naked
 flame.
The equipment should not be left unattended when the process is going
 on.
Greater care should be exercised when handling ignitable or organic substances.

Check setup before starting the process.
9

Ignition sources to be kept out of fume hood

Containers of flammable liquids to be kept sealed except during decanting.

Preventive maintenance should be done annually

Gas Cylinder
(Flammable)

Gas leak

Falling Gas cylinder

Fire

Body injuries
3
Heating Mantle
High Voltage DC
Power Supply for
Electro-spinning

High temperature

High voltage

Risk of burns due to touching hot surface

Low risk of electrocution due to spillage of water or solvents on heating mantle

Electric Shock
2
3
3
3
9

Store outside the laboratory and well ventilated place

Must always be firmly secured with clamp and never left unsupported.

No chemicals are allowed to store together with the gas cylinders

Proper pressure limits for the compressed gas system must be observed to prevent leaks and explosions

Correct pressure regulator to be used

Check gas leak regularly

Always use smaller cylinder if possible
6

Do not leave heating mantle switch on
 when unattended.
Do not place near flammable/volatile/explosive
 substances.
Ensured the apparatus is electrically checked on regularly.
2 6

Student must be guided by trained lab
 staff

Always discharge the load after each operation

Homogenizer

Contact with rotating shaft, homogenizer head

Irritating noise

High power

Cuts/Injuries

Elevated noise affect hearing

Exposure to hazardous materials
3
Hot Bead Sterilizers

Heat
Hot plate

Burns 2

High temperature

Risk of burns due to touching of hot plate

Low risk of fire due to ignition of oil or chemicals being heated

Low risk of electrocution due to spillage of water or solvents on hot plate.
3
3
2
3
9

Training in safe operation and guided by trained lab staff

Protected goggles must be worn.

Chamber must be fully closed when in use.

Switch off when cleaning.

Hearing protection is required for longer time or higher speed.

Reduce operation time and speed if possible.
4

Metal ware must not be left too long in the hot bead sterilizer.
9

Thermal gloves must be worn if necessary

Do not leave hot plate switch on when unattended.

Do not place the hot plate near flammable/volatile/explosive
 substances.
Avoid heating oil bath or chemicals to
 too high temp
Ensured the apparatus is electrically checked on regularly.

HPLC
Incubator
Infrared Lamp
Laminar Flow

Hazardous substances including flammable, irritant solvents used and testing materials

Exposure to hazardous substances

Fire can occur in the present of ignition sources
3

Heat

Danger of fire with low flash points chemicals


Burns
Fire

Heat

Explosive if it contacts with water as the lamp tends to be very hot when in use.

Exposure to UV light

Microorganisms

Burns

Injuries

Burns

Infections
2
3
3
3
2
3
3
9

Student must be guided by trained staff

Awareness of possible exposure to hazardous substances

No ignition sources or hot plate near the equipment.

Routine maintenance should be done

Preventive maintenance should be done annually
4

Do not place incubator near volatile/flammable/explosive substances

Thermal gloves should be worn if the
9 temperature setting is above 45 deg C

Do not allow contact with water and place away from the water sources.

Switches should turn off after use.
9

Users must be trained and strictly follow safety laboratory practices.

Do not stand near the hood when UV light is on

Turn off UV light before removing the
 front covers
Do not use flammable/volatile/explosive
 substances in the unit
Daily maintenance by lab staff is essential.

Preventive maintenance should be done annually

Laser
Microwave Oven

Burns

Leakage of Microwave radiation

Burns

Pose hazard to health
3
Milling Machine
Optical fibres
Optical Tweezers

Laser Radiation

Hazardous voltages

Cutters

Eye Damage

Electric Shock

Injuries

Broken fibres pieces are sharp and could penetrate the skin

Laser Radiation

Injuries

Eye Damage
3
3
3
3
3
3
2
2
3
9

Wear laser safety Eye goggles.

Class 3b laser users to be guided by licensed lab staff

The laser apparatus when not in use should be locked and is accessible only to authorized lab staff.
9

Thermal gloves must be worn.

Bottles/containers should not be closed tightly.

Seals to be checked regularly
 Stay at least an arm’s length away from the front of an operating microwave oven
6

Student must be guided by trained lab staff

Materials to be milled must be held securely.

Eye protection must be worn

After switching off the machine, always wait at least 5 minutes before removing the rear cover to access the electrical panel
6

Handle optical fibres as glass.

Fibres should be disposed carefully and not left lying on the bench.
9

Wear laser safety Eye goggles.

Student must be guided by trained lab staff

Oven –Dry Heat

High temp
Pump

Electric shock
Scalpels/Blades/Syringe needles

Sharp objects

Injuries

Cuts

Puncture

Burns

Fire



3
3
3
3
2
3
3
3
9

Wear thermal gloves when removing hot items from the oven

Never use oven to evaporate flammable or combustible chemicals

Do not place the oven near volatile/flammable/explosive substances.
6

Strictly follow the instructions.

Lab staff guided is essential
9

Lab staff supervision is essential

To emphasize the danger of sharp objects to students.

Used sharps should not be bent or broken, and must be disposed into sharp waste container

Syringe with needle is only allowed to use when necessary and lab staff
 supervision is required

Issue to students only when necessary
Only lab staff are accessible to the storage box.
9





Shaking Incubator

Spilling of Chemicals or Microorganisms

Broken glass

Chemicals or microorganism contamination

Cuts
3
Soldering irons

Electric shock

Heat from melted component.

Burns

Injuries
Sonicator

Vibration

Hearing impairment
3
Thermometer
UV transilluminator

Mercury vaporized from broken
 thermometer.
Broken pieces

UV radiation

Toxic vapor inhalation from broken
 thermometer
Cuts

Harmful to eyes

Skin burns
3
3
3
2
3
2
3
3
6

Flasks or bottles should not be overfilled or over-tighten.

Ensure the flasks or bottles are secured

Place objects on the platform in a stable condition

Do not overload the shaker or exceed the equipment’s specification.
9

Lab staff should ensure the equipment and cables are maintained in good conditions

Suitable eye protection should be worn

Students are not allowed to operate this process
6

Student must be guided by trained lab staff

Minimize sample sonication time

Perform in fume hood if necessary

Ear protection if needed
9

All breakage, accidents and spillage must be reported immediately to
 teachers or lab staff.
Follow mercury spillage SOP
9


Lab staff supervision is essential.
Full face shield should be worn

UV lamp must be off after use

Vacuum Concentrator
Vacuum Oven




Toxic or flammable vapours
Toxic volatile substance

Implosion
Heat
Broken glass





Inhalation of toxic vapours
Fire if presence of ignition sources.
Burns
Cut
Hazardous fume evolved

Intoxication

Explosion
3
3
2
3
6

The equipment must not be used to evaporate low flash points substances.

When evaporating flammable liquids,volatile liquids or explosive liquids , a cooling trap should be used to ensure condensation and separation of the vapour

Student must be guided by trained lab staff
9

Lab staff supervision is essential

Thermal gloves must be worn

Do not place or dry explosive, flammable, corrosive and combustible materials around or in the oven.

Do not seal the glassware

Door gasket will degrade at high temp
Vortex mixer
Water-Bath



Samples leak
Electric shock
Heat

Exposure to

 hazardous substances
Injuries
Scalding
2
2
2
2

Emergency procedures and first aid kit must be in place.

Lab users to be aware of where nearest firefighting equipment is located & emergency phone number.
 after some time, check frequently.
Check Vacuum pump frequently
4

Users are to ensured tubes sealed properly
4

Fill the bath with water to the recommended height.
 “HIGH TEMP, DO NOT TOUCH” sign should be displayed if the temperature
 setting is higher than 60 deg C.
Off the water-bath if not in use.

Equipment Chemicals and Processes
1. Experiments assessment
Possible hazardous

Risks of operating equipment
Risk
Level
2. Use of biohazardous substances
3. Use of Hazardous chemicals

Exposure to infectious substances

Acute or chronic
Infections

Exposure to hazardous chemicals

Acute or chronic
Health effects from chemical exposures
Measures to reduce the risks posed by hazards

Lab coat and gloves must be worn at all times when working in lab

To modify the experimental procedure if higher risk is involved.

Students must read and understand the safety rules before doing experiment


All students users must be trained before commencing of their experiments.
Proper operation of equipment should be followed.

Users must always follow safety regulations strictly

Lab coat and gloves must be worn at all times when working in lab

Restrict work to the BSL-1 microorganisms. Tissues, blood or body fluids from human or vertebrates are not allowed to use.

Microorganisms of unknown pathogenicity or from unknown sources must not be used

All used apparatus contaminated with microorganisms and culture media must be autoclaved after the experiment

Students must be trained ,strictly follow safety laboratory practices and
 guided by trained lab staff

Associated signs use to demonstrate hazards.
Work to be done in biosafety cabinet

Washing of hands after process is completed

Disposal as per Laboratory Safety Manual.

Lab coat and gloves must be worn at all times when working in lab

Assessment prior to use if this chemical must be used or if a less dangerous chemical can be substituted.

Students must be trained and strictly follow safety laboratory practices

MSDS must be accessed prior to usage

Associated signs use to demonstrate hazards.

Prepare chemicals in the appropriate work station e.g. fume hood

Washing of hands after process is completed

Disposal as per Laboratory Safety Manual