Discussion Questions Lab 2: Purification of gDNA from Fish Tissue (CMB)
CLASS SET
Directions: Answer the following questions on YOUR OWN PAPER and in COMPLETE SENTENCES.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
Why did you centrifuge your sample tubes in step #3?
After centrifugation, what was in your tube? Where is your DNA?
Why did you have to be careful not to disturb the pellet?
What 3 reagents did you add to the supernatant (steps 9-12)?
Describe the role of each of these reagents.
After centrifugation in step #16, where is your DNA? What is in the collection tube (be specific)?
How did you ensure that the silica matrix was not disturbed?
What was the purpose or function of wash buffer 1 / wash buffer 2?
What was the purpose of adding distilled water to the spin column in step #29?
After centrifugation in step #29, where is your DNA?
In your own words, summarize steps #31-33. What did you do?
In your opinion was this lab valuable? Did you understand it? Were there any areas of
confusion?
13. Did you feel prepared to successfully execute all the steps listed in the protocol?
Discussion Questions Lab 2: Purification of gDNA from Fish Tissue (CMB)
CLASS SET
Directions: Answer the following questions on YOUR OWN PAPER and in COMPLETE SENTENCES.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
Why did you centrifuge your sample tubes in step #3?
After centrifugation, what was in your tube? Where is your DNA?
Why did you have to be careful not to disturb the pellet?
What 3 reagents did you add to the supernatant (steps 9-12)?
Describe the role of each of these reagents.
After centrifugation in step #16, where is your DNA? What is in the collection tube (be specific)?
How did you ensure that the silica matrix was not disturbed?
What was the purpose or function of wash buffer 1 / wash buffer 2?
What was the purpose of adding distilled water to the spin column in step #29?
After centrifugation in step #29, where is your DNA?
In your own words, summarize steps #31-33. What did you do?
In your opinion was this lab valuable? Did you understand it? Were there any areas of
confusion?
13. Did you feel prepared to successfully execute all the steps listed in the protocol?