Business Address
Strotskaya Alexandra
40 Fedora Poletaeva street
109378, Moscow, Russia
[email protected]
Date of Birth
November 15, 1988
2013 – present: Junior Researcher, Institute of Molecular Genetics, RAS. Supervisor
Dr. K. Severinov.
2011 – 2013: Junior Researcher, Winogradsky Institute of Microbiology, RAS.
Supervisor Dr. A. Letarov.
2006 – 2011: Specialist of Science in Biophysics, Department of Biophysics,
Moscow State Academy of Veterinary Medicine and Biotechnology, Major GPA: 5.0/5.0;
Overall GPA: 4.8/5.0.
Research Experience
2013 - present: Laboratory of genetic regulation of prokaryotic mobile genetic
elements, Institute of Molecular Genetics, RAS, PhD student (Research group leader
Dr. K. Severinov)
Project: The dynamics of the interaction of E.coli cells and mobile genetic elements in
the presence of CRISPR/Cas system.
2011 – 2013: Group of microbial viruses, Institute of Microbiology, RAS, PhD student
(Research group leader Dr. A. Letarov).
Project: The temporal dynamics of a viral component in symbiotic microbial community.
2009 – 2011: Group of microbial viruses, Institute of Microbiology, RAS, stager
(Research group leader Dr. A. Letarov).
Project: Isolation of minor enterobacteriophages from animal feces and their
Work Experience:
Work with bacteriophages: preparing stocks of bacteriophages, purification of phages
DNA from liquid cultures, assaying the DNA content in bacteriophages stocks and
lysates by agarose gel electrophoresis, purification of bacteriophage particles, including
centrifugation through CsCl gradients, purification of bacteriophage particles by
centrifugation through glycerol step gradients, purification of bacteriophage particles by
Molecular cloning: preparation of vector DNA, preparation of DNA to be cloned,
creation of recombinant DNA with DNA ligase, preparing of competent cells,
introduction of recombinant DNA into host organism by chemical transformation and
electroporation, screening for clones with desired DNA by PCR analysis and restriction
analysis, screening for expression of desired genes with SDS-PAGE.
Electron microscopy: preparation grids for electron microscopy, negative staining of
total phage samples with uranium acid, work with transmission electron microscope
Publications: Isolation and characterization of a novel indigenous intestinal N4-related
coliphage vB_EcoP_G7C. Kulikov E, Kropinski AM, Golomidova A, Lingohr E, Govorun
V, Serebryakova M, Prokhorov N, Letarova M, Manykin A, Strotskaya A, Letarov
A.Virology. 2012 May 10;426(2):93-9. doi: 10.1016/j.virol.2012.01.027. Epub 2012 Feb
Scientific Interests
My scientific interests focus on molecular and biochemical mechanisms of CRISPRadaptation for linear DNA.