CIRRICULUM VITAE Name Business Address Strotskaya Alexandra 40 Fedora Poletaeva street 109378, Moscow, Russia Phone 8-903-782-42-18 E-mail [email protected] Date of Birth Citizenship November 15, 1988 Russia Education 2013 – present: Junior Researcher, Institute of Molecular Genetics, RAS. Supervisor Dr. K. Severinov. 2011 – 2013: Junior Researcher, Winogradsky Institute of Microbiology, RAS. Supervisor Dr. A. Letarov. 2006 – 2011: Specialist of Science in Biophysics, Department of Biophysics, Moscow State Academy of Veterinary Medicine and Biotechnology, Major GPA: 5.0/5.0; Overall GPA: 4.8/5.0. Research Experience 2013 - present: Laboratory of genetic regulation of prokaryotic mobile genetic elements, Institute of Molecular Genetics, RAS, PhD student (Research group leader Dr. K. Severinov) Project: The dynamics of the interaction of E.coli cells and mobile genetic elements in the presence of CRISPR/Cas system. 2011 – 2013: Group of microbial viruses, Institute of Microbiology, RAS, PhD student (Research group leader Dr. A. Letarov). Project: The temporal dynamics of a viral component in symbiotic microbial community. 2009 – 2011: Group of microbial viruses, Institute of Microbiology, RAS, stager (Research group leader Dr. A. Letarov). Project: Isolation of minor enterobacteriophages from animal feces and their characteristics Work Experience: Work with bacteriophages: preparing stocks of bacteriophages, purification of phages DNA from liquid cultures, assaying the DNA content in bacteriophages stocks and lysates by agarose gel electrophoresis, purification of bacteriophage particles, including centrifugation through CsCl gradients, purification of bacteriophage particles by centrifugation through glycerol step gradients, purification of bacteriophage particles by pelleting/centrifugation. Molecular cloning: preparation of vector DNA, preparation of DNA to be cloned, creation of recombinant DNA with DNA ligase, preparing of competent cells, introduction of recombinant DNA into host organism by chemical transformation and electroporation, screening for clones with desired DNA by PCR analysis and restriction analysis, screening for expression of desired genes with SDS-PAGE. Electron microscopy: preparation grids for electron microscopy, negative staining of total phage samples with uranium acid, work with transmission electron microscope Jeol-100X. Publications: Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C. Kulikov E, Kropinski AM, Golomidova A, Lingohr E, Govorun V, Serebryakova M, Prokhorov N, Letarova M, Manykin A, Strotskaya A, Letarov A.Virology. 2012 May 10;426(2):93-9. doi: 10.1016/j.virol.2012.01.027. Epub 2012 Feb 15. Scientific Interests My scientific interests focus on molecular and biochemical mechanisms of CRISPRadaptation for linear DNA.