Introduction to Image Fusion There have been geographic studies that combine the principle component transformation with image fusion for geographic purposes. In these studies, the images were broken into different color bands that served as the principle components. [1-6] The chemical image map produced by ToF-SIMS uses the intensity of brightness as a means to represent the quantitative information. Using some examples from geography, there are two or three methods that combine the use of the principle component transform with that of image fusion. Deciding which method is appropriate depends on the data. When the objective is to create a hybrid image that would quantify information, there are certain assumptions that must be made. There has to be a degree of correlation present between the multi-spectral band and the brightness on the intensity scale. The method results in the formation of super-pixels that have the same field of view as the original multi-spectral pixels. Each super-pixel is comprised of sub-pixels that are re-sampled at the same field of view. The equation used to calculate the hybrid, high-resolution pixels’ brightness is: π ′ π·πΆπππ£π π·πΆππ π·πΆπ =( )∑ = π·πΆπ π·πΆπππ£π π (1) π=1 ′ π·πΆπ′ is the resolution value given to the hybrid sub-pixels of any spectral band. π·πΆπππ£π is the sum of all the values given to all the hybrid pixels, divided by the number of sub-pixels in the superpixel. π·πΆπ is the resolution value given to the super-pixel. π·πΆπππ£π is the mean digital count in the peak of interest for the high-resolution pixels of interest. [1] Low Resolution, High Information Image Fusion High Resolution, Low Information Hybrid Image Supplemental Figure 1: Schematic of Image Fusion FLIR Image Radar Image Fused Supplemental Figure 2: Image fusion satellite and radar image example. See reference for more details and basic advantages for this fusion technique. [8] (a) (b) (c) Supplemental Figure 3: A low resolution multi-spectral image (RIS-1C LISS-III) with a resolution of 23.5 meters (a), a high resolution panchromatic image (IRS-1C PAN) with a resolution of 5.8 meters (b), and a hybrid image that is the result of PCA applied to the image fusion of (a) and (b). [9] Supplemental figure 3 is an example of image fusion done in combination with a common multivariate statistic technique, PCA. This example shows a common application in satellite imaging while also showing some of the adverse effects of the pre-treatment and normalization necessary to perform PCA. (For more specific details see reference [9].) 1.7 Introduction to Fast Fourier Transform (FFT) Fast Fourier Transform (FFT) is an algorithm developed by Cooley and Tukey. [3, 4] This algorithm increased the speed of the typical Discrete Fourier Transform (DFT), which was extremely useful in transforming data into frequency domains. However, DFTs produce matrices with large amounts of redundancy, due to the symmetry and periodicity in the FT code matrix. [3] The equation for the DFT is as follows: [4] π−1 πΉ(π£π ) = ∑ π(π‘π ) (2) π=0 Here, πΉ(π£π ) is the frequency-domain (complex) spectral data point, from N (real or complex) time-domain data points [f(tn), n=0, 1, 2, … n-1], and where πΉππ = ππ₯π ( −π2πππ π ), with Cooley and Tukey’s adaptation, the N-point time-domain, is divided in two halves, and the discrete Fourier transform is performed on each half. The results are then recombined by multiplication, such that each subset is reduced to two data points. With this alteration, Equation 2 becomes the following equation: [4] π−1 πΉ(π) = ∑ ππΉ πππ‘(π) ; n = 0,1,2, … , N − 1 (3) π=0 The index, N, has been introduced into the Fourier “code” element NFnm, in order to specify that discrete FT is applied to an N-point data set. [4] 4 1.0 0.8 0.6 0.4 0.2 185 190 195 200 Mass (u) (a) TaO 1e3 1e2 1e-1 ^180Ta^18O 1e0 196 197 198 Ta^18O 1e1 ^180TaO Intensity (counts) x10 199 (b) Supplemental Figure 4: (a) Negative ion spectrum produced by the ToF-SIMS from the Cameca grid sample. (b) ratio produced from the characteristic Ta oxide components. 4 2.0 1.5 1.0 0.5 170 175 180 185 190 Mass (u) (a) Ta 1e3 1e2 1e1 1e0 ^180Ta Intensity (counts) x10 1e-1 180.50 180.75 181.00 181.25 181.50 (b) Supplemental Figure 5: (a) The positive ion spectra produced by the ToF-SIMS from the Cameca grid sample. (b) The ratio from the representative isotopes. (a) (b) Supplemental Figure 6: FFTs of SIMS and SEM Images. (a) The FFT of the SIMS image. (b) The FFT of the SEM image. (a) (b) (c) (d) (e) (f) Supplemental Figure 7: Segments of the FFT corresponding to characteristic portions of the A-grid ToF-SIMS image (a, c, e), and their respective inverse FFT segments (b, d, f). (a) (b) (c) (d) (e) (f) Supplemental Figure 8. Segments of the FFT corresponding to characteristic portions of the A-grid SEM image (a, c, e), and their respective inverse FFT segments (b, d, f). a b c d Supplemental Figure 9: Inverse FFT Analysis of Background and Edge Effects. (a) The SIMS FFT of the background / edge effect. (b) The SIMS Inverse FFT of the background / edge effect. (c) The SEM FFT of the background / edge effect. (d) The SEM Inverse FFT of the background / edge effect. In supplemental figure 7 and 8, an inverse FFT analysis was performed to validate the FFT results in supplemental figure 6. The inverse FFT analysis allowed specific frequency domains from the FFT (see supplemental figure 7(a,c,e) and 8(a,c,e)) to be selected and analyzed. Thus, this technique provided validation by demonstrating that the specific domains were characteristic of the same portion of the letter "A" in both the SEM and ToF-SIMS images. For both the SEM and ToF-SIMS images a distinct frequency domain could be attributed to edge and noise and is imaged in figure supplemental figure 9. As can been seen in Supplemental figure 7 and 8 each distinct portion of the letter "A" is identified. For example, Figure 7e shows the bright vertical band (frequency domain) found in the FFT of the ToF-SIMS image. Figure 7f shows the corresponding inverse FFT, which clearly shows the crossbar of the letter "A" in the ToF-SIMS image. By comparing this portion of both the SEM and ToF-SIMS FFTs, which clearly display the crossbar feature of the A-grid, the width discrepancy discussed above was again apparent. There was one additional feature found in both FFT images (Supplemental figure 9, Supplemental Information). When the inverse of this feature was examined, it could not be attributed to any portion of the letter “A”, however it is related to edge effects and background present in both images. With the results from FFT and inverse FFT analysis the expected spatial correlation that should be present when using two types of analyses to obtain images from the same copper TEM A-Grid was evident. References 1) Schott, J. R., Remote Sensing: The Image Chain Approach. Oxford University Press: New York, 1997. 2) Sodhi, R. N. S., Time-of-flight secondary ion mass spectrometry (ToF-SIMS):- versatility in chemical and imaging surface analysis. Analyst, 2004, 129, 483-487. 3) Bracewell, R.N. The Fourier Transform and Its Applications. 2nd Ed. McGraw-Hill, Inc.; 1986. 4) Marshall, A.G. and Verdun, F.R. Fourier Transforms in NMR, Optical, and Mass Spectrometry: A User’s Handbook. Elsevier, 1990. 5) Sanjeevi, S. , Vani, K., Lakshmi, K., Comparison of Conventional and Wavelet Transform Techniques for Fusion of IRS-IC LISS-III and PAN Images; Remote Sensing Conference, 2001. 6) Schetslelaar, Earnst M. On preserving spectral balance in image fusion and its advantages for geological image interpretation. Photogramm Eng Rem S, 2001, 67, 925-934. 7) Webster, R.; Oliver, M., Geostatistics for Environmental Scientist. John Wiley & Sons: New York, 2001. 8) http://vision.arc.nasa.gov/personnel/al/hsr/fusion/fr.gif 9) Sanjeevi, S. , Vani, K., Lakshmi, K., Comparison of Conventional and Wavelet Transform Techniques for Fusion of IRS-IC LISS-III and PAN Images; Remote Sensing Conference, 2001. 10) http://www.answers.com/topic/correlation-coefficient 11) http://www.geovariances.com/software/exploratory-data-analysis-ar0065.html 12) Goovaerts, P., Geostatistics in soil science: state-of-the-art and perspectives. Geoderma 1999, 89, 1-45. 13) Goovaerts, P. Geostatistics for Natural Resources Evaluation; Applied Geostatistics Series; Oxford University Press: New York: NY, 1997; pp 1-483.