The absolute photolysis rates of estrone and estrone 3-methyl ether were determined through the use of an iodide-iodate actinometer. The rate of photons absorbed by the iodide-iodate solution when exposed to three different light sources (photoreactor, handlamp, and fluorimeter) was measured with UV-Vis spectroscopy. Using this photon rate, the photolysis rates of estrone in a variety of solvents was followed with gas-chromatography/mass spectrometry and was found to differ based on solvent. Estrone and estrone 3-methyl ether were also shown to photodegrade at different rates when irradiated in the same solvent.