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Supplementary Material: Genetic Diversity and Population
Structure of the Threatened Freshwater Catfish, Tandanus
tandanus, in Victoria, Australia
ERIN HILL*, BRETT INGRAM ✝, MEAGHAN ROURKE‡, JOHN
MITCHELL* AND JAN M. STRUGNELL*
*
Department of Genetics, La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, Victoria
3086, Australia
✝
Department of Environment and Primary Industries, Alexandra, Victoria 3714, Australia
‡
Department of Primary Industries, Narrandera Fisheries Centre, P.O. Box 182, Narrandera, NSW, 2700,
Australia
Corresponding Author: Jan M. Strugnell, Department of Genetics, La Trobe Institute for Molecular Sciences,
La Trobe University, Melbourne, Victoria 3086, Australia. E-mail: J.Strugnell@latrobe.edu.au Telephone:
94793663
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Supplementary Material
Table S1 Tajima’s D and Fu’s Fs statistics, and P values. No population was found to have undergone
significant population size expansion or contraction
Population
Tajima’s D
P Value
Fu’s Fs
P Value
Goulburn
0
1
0
0
Mallee
1.42
0.93
1.17
0.73
Little Murray
0.62
0.74
1.92
0.88
Gunbower
1.24
0.9
3.11
0.92
Wimmera
-1.21
0.08
1
0.53
Avoca
0
1
0
0
Loddon
0.36
0.68
3.82
0.95
ALL SITES
1.82
0.96
2.72
0.86
Table S2 Pairwise ΦST statistics for three major geographical locations of T. tandanus in Australia. Significance
is indicated in bold (<0.05)
(B)
Victoria
NSW Coast
Victoria
*
NSW Coast
0.08
*
NSW Murray
Darling
0.11
0.1
NSW Murray
Darling
*
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Table S3 Null corrected sample size (n), number of private alleles (NPA), allelic richness (AR), observed and
expected heterozygosity (HO and HE), FIS values and Wilcoxon test for excess heterozygosity for each Victorian
population of T. tandanus, based on microsatellite data. Negative FIS values indicate an increase of
heterozygotes due to outbreeding, whilst positive F IS values indicate an excess of homozygotes due to
inbreeding. Bold values show significance (Wilcoxon test), indicating a population has recently undergone a
genetic bottleneck
Populations
n
NPA
AR
Avoca
29
0
2.61
Goulburn
28
2
Little
Murray
9
Loddon
HO
HE
FIS
Wilcoxon
Test
0.435 0.411
-0.052
0.019
2.71
0.513 0.442
-0.150
0.0078
1
4.34
0.637 0.580
-0.107
0.019
30
0
3.29
0.430 0.452
0.057
0.055
Mallee
44
10
5.32
0.648 0.681
0.030
0.191
Gunbower
44
3
3.72
0.483 0.494
0.004
0.098
Wimmera
34
1
4.49
0.647 0.661
0.015
0.0039
ALL SITES
218
-
3.78
0.542 0.532
-0.026
-
Table S4 Null corrected pairwise FST statistics for each population in Victoria, based on microsatellite data.
Bold values indicate significance level at 0.05; asterisk represents significance after Bonferroni correction
(0.002381)
Avoca
Goulburn
Little
Murray
Loddon
Mallee
Gunbower Wimmera
Avoca
*
Goulburn
0.33*
*
Little Murray
0.20*
0.22*
*
Loddon
0.02*
0.27*
0.14*
*
Mallee
0.21*
0.15*
0.07*
0.16*
*
Gunbower
0.36*
0.18*
0.23*
0.30*
0.12*
*
Wimmera
0.28*
0.18*
0.09*
0.22*
0.04*
0.17*
*
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Fig. S1 (a) Null corrected DAPC plot for all samples, assigned into four genetic clusters (K=4). Pie charts
represent the contribution of each population to the cluster. (b) Null corrected DAPC plot with Loddon and
Avoca samples removed, samples were assigned into three genetic clusters (K=3). Pie charts represent the
contribution of each population to the cluster
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Fig. S2 (a) Null corrected Structure plot (K=3) for all Victorian samples. (b) Null corrected Structure plot (K=2)
with translocated Avoca and Loddon populations removed. This Structure plot contained a Delta K value of
122.46 (c) Null corrected Structure plot (K=4) with translocated Avoca and Loddon populations removed. This
Structure plot had the second largest Delta K value of 27.25. Each population is listed at the bottom of the plot;
individual water bodies are numbered as follows; 1) Amphitheatre Reservoir 2) Major’s Creek 3) Nagambie
Lake 4) Tahbilk Lagoon 5) Little Murray River 6) Centenary Reservoir 7) King’s Billabong 8) Sandilong Creek
9) Cardross Lake 10) Turners Lagoon 11) Gum Lagoon 12) Phyland Lagoon 13) Safe Lagoon 14) Southern
Wimmera 14) Northern Wimmera