Light-dependent Gene Activation in Aspergillus nidulans is Strictly Dependent
on Phytochrome and Involves the Interplay of Phytochrome and White-collarregulated Histone H3 Acetylation
Maren Hedtke1, 3, Stefan Rauscher1, 3, Julian Röhrig1, 3, Julio Rodriguez2, Zhenzhong
Yu1 and Reinhard Fischer1*
Figure S1: Deletion of ccgA A Southern Blot of the ccgA-deletion strain. Digestion
with BglII; expected band after hybridization with the ORF is 1.8 kb, after
hybridization with the left border (LB): 1.8 kb for WT and 1.01 kb for the deletion
strain, for the right border (RB): 1.8 + 1.3 kb for WT and 1.3 kb for the deletion strain.
B Deletion of ccgA shows no discernible phenotype with respect to colony size or
sporulation.
Figure S2: BiFC experiments of FphA and VeA with GcnE and HdaA. GcnE and
HdaA are fused to the N-terminus of YFP, FphA and VeA are fused to the Cterminus. Spores were inoculated in medium containing 2 % glycerol and grown over
night at 28°C.
Figure S3: Recomplementation of the gcnE deletion and phenotypes of the
hdaA- and adaB-deletion strains. The deletion strain of gcnE (upper panel) was
recomplemented with alcA::GFP::gcnE restoring wild type phenotype when growing
on inducing medium (containing 2 % glycerol). Deletion of hdaA (middle panel)
shows growth comparabale to wild type but less conidiation. The adaB-deletion strain
(lower panel) grows significantly slower than wild type and conidiation is impaired.