Enzyme Function Lab
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SAFE WORK PROCEDURE
LOCATION
VMC
WRITTEN BY: APPROVED BY:
Science Team
DATE CREATED
WS&H
LAST REVISION
June 27, 2014
New
PERSONAL PROTECTION EQUIPMENT (PPE)
Safety glasses or face shield must be worn at all
times in work areas.
Long and loose hair must be tied back
Appropriate footwear must be worn. Shoe must
be fully enclosed. No open toed shoes.
Close fitting/protective clothing must be worn.
Remove strings hanging from pullovers/sweaters.
Rings and jewelry (long necklaces / bracelets,
etc.) must not be worn.
HAZARDS PRESENT
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Chemical burns
Absorption of Chemicals.
Inhalation of Chemicals.
Ingestion of Chemicals.
Slips / trips & falls
Chemical hazards
ADDITIONAL
REQUIREMENTS
APPARATUS / MATERIALS
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Hydrogen peroxide
Test tubes
Graduated cylinder
Manganese dioxide
Chalk
Hamburger
Liver (fresh and boiled)
Spinach
Potato
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Equipment orientation
WHMIS training
SAFE WORK PROCEDURE
1. Don all personal protective equipment including: safety glasses, & protective nitrile gloves
(where required). Ensure all loose clothing is either removed or tied back. Remove any jewelry
and tie back long hair.
2. Measure 2ml of hydrogen peroxide in a graduated cylinder and pour into a test tube. Add to the
test tube a pinch of manganese dioxide. Describe the reaction. To avoid irritation, be careful
handling hydrogen peroxide.
3. Pour 2ml of hydrogen peroxide into each of five test tubes.
4. Place a pre-cut piece of chalk, hamburger, liver, spinach, and potato into the separate test
tubes. Record your observations.
5. Take a small piece of boiled liver and place into a test tube with 2ml of hydrogen peroxide.
Place a piece of fresh liver into another test tube with 2ml of hydrogen peroxide. Record your
observations.
SAFE WORK PROCEDURE
REGULATORY REQUIREMENTS
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WS&H Act W210, Section 4, 5
Mb. Regulations 217/2006,
Part 16, (Machines / Tools & Robots) Sections 16.1-16.18)
Part 35, (WHMIS Application)
Part 36, (Chemical & Biological Substances Application)
Enzyme Function Lab
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