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Supporting Information, Tables and Figures
Supplementary Tables
Table S1
Sample locations for Seriatopora spp. from the Palm Islands and Lizard Island,
GBR. Habitat: S – sheltered, E – Exposed; N: total # colonies sampled per location; Msats:
collections used for microsatellite analysis; mtDNA: collections surveyed with new mtDNA
marker; Sym ITS2: collections used for Symbiodinium identification; XX: also sequenced for
mtDNA.
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Table S2
Published sequences used in mtDNA sequence analysis. Target Spp.: named
species targeted for study; NTOT: total # sequences from the study; Location surveyed: country of
collection; NLOC: # sequences from each location; Sequence length (bp): total length of published
sequences including indels.
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Table S3
Descriptive statistics for ten microsatellite loci and thirty populations of five
putative Seriatopora species from the Palms Islands and Lizard Island regions of the Great
Barrier Reef. Color-coded and labeled by putative species. N: number of individuals; NA: #
alleles; HO: observed heterozygosity; HE: expected heterozygosity: FIS: inbreeding coefficient
(Weir 1994).
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Table S4
Summary of nineteen mtDNA control region haplotypes found in the genus
Seriatopora. mtDNA amplicon: in this study, small (no indel), medium (24 bp indel), large (51
bp indel).
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Supplementary Figures
Figure S1
Principal component analysis calculated on pairwise codominant genotypic
distances among individuals. Key at bottom indicates genetic clusters from Fig. 1 (colors) and
region of samples (shapes).
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Figure S2
Comparative patterns in genetic separation by distance for 5 putative species of
Seriatopora. A) Geographic distances among 30 populations of Seriatopora versus pairwise FST
values for members within each genetic cluster; key to right. Linear regression values for
populations of ShA, ShB, Sc and between all genetic clusters combined indicated by colored
boxes. Companion figure to Fig. 4 in the main text.
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Figure S3
Sequence alignment of mitochondrial primers ShMT662F and ShMT960R for
Seriatopora spp. The three amplicon sizes represent all of the 19 mitochondrial haplotypes
detected for seriatoporid corals: Small (248 bp, no indels); Medium (272 bp, 24 bp indel);
Large (299 bp, 51 bp indel). Red type indicates the indels that distinguish types medium and
large from the small haplotype.
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Figure S4 Comparison of genetic structure in S. hystrix with and without separation of cryptic
species. A. PCA of FST values for 19 populations represented by sampling site (labeled by
population code) and including all four S. hystrix putative species combined. Symbols represent
habitats and regions: circle – sheltered, square – exposed, star – intermediate habitat (W. Lizard),
hollow – Lizard Island, solid – Palm Islands. B. PCA of FST values for populations of ShA only.
C. PCA of FST values for populations of ShB only.
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