AP Biology Syllabus 2016 Unit 5: Bioenergetics Chapters 8, 9, & 10 Date Class Discussion Topic/Activity Objectives/Learning Targets (LTs) Monday January 4 Chapter 8 notes – Thermodynamics & Entropy Chapter 8: An Introduction to Metabolism (p. 141- 150) 1. I can explain that life is a highly ordered system. a. Order is maintained by constant free energy input into the system b. Loss of order or free energy results in death. c. Increased disorder and entropy are offset by biological processes that maintain or increase order. 2. I can explain that living systems do not violate the second law of thermodynamics, which states that entropy increases over time. a. Order is maintained by coupling cellular processes that increase entropy (and so have negative changes in free energy) with those that decrease entropy (and so have positive changes in free energy). b. Energy input must exceed energy lost to entropy to maintain order and power cellular processes. c. Energetically favorable exergonic reactions, such as ATP ADP, that have negative change in free energy can be used to maintain or increase order in a system by being coupled with reactions that have a positive free energy change. 3. I can explain how energy-related pathways in biological systems are sequential and may be entered at multiple points in the pathway, such as: a. Krebs cycle b. Glycolysis c. Calvin cycle d. Fermentation Free Energy POGIL Tuesday January 5 Assignment (Unless otherwise noted assignments are due the next day class meets) Read chapter 8 pages 142 – 151 (material on Energetics…up to the enzyme material), take notes Watch Bozeman Videos: Gibbs Free Energy Life Requires Free Energy (this video makes more global connections but also hits on some of the learning targets) Gibbs Free Energy Activity http://ats.doit.wisc.edu/biology/lessons.htm ATP – the free energy carrier POGIL Wednesday January 6 Cellular Respiration Overview Glycolysis & Krebs Cycle (POGIL) Chapter 9: Cellular Respiration and Fermentation 1. I can explain how energy-related pathways in biological systems are sequential and may be entered at multiple points in the pathway, such as: a. Krebs cycle b. Glycolysis c. Fermentation 2. I can explain how heterotrophs capture free energy present in carbon compounds produced by other organisms. a. Heterotrophs may metabolize carbohydrates, lipids and proteins by hydrolysis as sources of free energy. b. Fermentation produces organic molecules, including alcohol and lactic acid, and it occurs in the absence of oxygen. 3. I can explain how mitochondria specialize in energy capture and transformation. a. Mitochondria have a double membrane that allows compartmentalization within the mitochondria and is important to its function. b. The outer membrane is smooth, but the inner membrane is highly convoluted, forming folds called cristae. c. Cristae contain enzymes important to ATP production; cristae also increase the surface area for ATP production. 4. I can explain how different energy-capturing processes use different types of electron acceptors, such as oxygen in Actively read, take notes chapter 9 Chapter 9 one-pager due Wednesday, January 13 Videos: Bozeman Science: Cellular Respiration Crash Course ATP and Respiration Optional Prezis: Cellular Respiration Cellular Energetics 5. 6. 7. Thursday January 7 Friday January 8 cellular respiration. I can explain how cellular respiration in eukaryotes involves a series of coordinated enzyme-catalyzed reactions that harvest free energy from simple carbohydrates. a. Glycolysis rearranges the bonds in glucose molecules, releasing free energy to form ATP from ADP and inorganic phosphate, and resulting in the production of pyruvate. b. Pyruvate is transported from the cytoplasm to the mitochondrion, where further oxidation occurs. c. In the Krebs cycle, carbon dioxide is released from organic intermediates, ATP is synthesized from ADP and inorganic phosphate via substrate level phosphorylation, and electrons are captured by coenzymes. d. Electrons that are extracted in the series of Krebs cycle reactions are carried by NADH and FADH2 to the electron transport chain. I can explain how the electron transport chain captures free energy from electrons in a series of coupled reactions that establish an electrochemical gradient across membranes. a. Electron transport chain reactions occur in mitochondria (cellular respiration). b. In cellular respiration, electrons delivered by NADH and FADH2 are passed to a series of electron acceptors as they move toward the terminal electron acceptor, oxygen. c. The passage of electrons is accompanied by the formation of a proton gradient across the inner mitochondrial membrane with the membrane(s) separating a region of high proton concentration from a region of low proton concentration. d. The flow of protons back through membrane-bound ATP synthase by chemiosmosis generates ATP from ADP and inorganic phosphate. e. In cellular respiration, decoupling oxidative phosphorylation from electron transport is involved in thermoregulation. I can explain how free energy becomes available for metabolism by the conversion of ATP ADP, which is coupled to many steps in metabolic pathways. Oxidative Phosphorylation/ETC Oxidative Phosphorylation (POGIL) AP exam registration Pre-lab AP Lab 6 Ch. 9 one-pager due tomorrow AP Lab 6 Report due Tuesday, January 19 Mastering Biology Chapter 9 quiz by 11:59pm Quiz – ch. 8/9 Monday January 11 Modeling Cellular Respiration Activity Pre-Lab Quiz AP Lab 6 – Cellular Respiration (Guided Inquiry) Tuesday January 12 Wednesday January 13 Thursday January 14 AP Lab 6 – Cellular Respiration (Student Design) Fermentation Anaerobic Respiration in Yeast Lab Bioflix – Cellular Respiration Cellular Respiration Summary Table Walk through CR cards Read, take notes Chapter 10 One-pager due Wednesday, January 20 Friday January 15 Quiz – ch. 9 Photosynthesis Overview Light Reactions Chapter 10: Photosynthesis (sections 10.1-10.3 only) 1. I can explain how energy-related pathways in biological systems are sequential and may be entered at multiple points in the pathway, such as: a. Calvin cycle 2. I can explain how autotrophs capture free energy from physical sources in the environment. a. Photosynthetic organisms capture free energy present in sunlight. b. Chemosynthetic organisms capture free energy from small inorganic molecules present in their environment, and this process can occur in the absence of oxygen. 3. I can explain that photosynthesis first evolved in prokaryotic organisms; scientific evidence supports that prokaryotic (bacterial) photosynthesis was responsible for the production of an oxygenated atmosphere; prokaryotic photosynthetic pathways were the foundation for eukaryotic photosynthesis. 4. I can explain how chloroplasts are specialized organelles found in algae and higher plants that capture energy through photosynthesis. a. The structure and function relationship in the chloroplast allows cells to capture the energy available in sunlight and convert it to chemical bond energy via photosynthesis. b. Chloroplasts contain chlorophylls, which are responsible for the green color of a plant and are the key light-trapping molecules in photosynthesis. There are several types of chlorophyll, but the predominant form in plants is chlorophyll a. c. Chloroplasts have a double outer membrane that creates a compartmentalized structure, which supports its function. i. Within the chloroplasts are membrane-bound structures called thylakoids. ii. Energy-capturing reactions housed in the thylakoids are organized in stacks, called grana, to produce ATP and NADPH2, which fuel the carbonfixing reactions in the Calvin cycle. iii. Carbon fixation occurs in the stroma, where molecules of CO2 are converted to carbohydrates. 5. I can explain how different energy-capturing processes use different types of electron acceptors, such as NADP+ in photosynthesis. 6. I can explain how the light-dependent reactions of photosynthesis in eukaryotes involve a series of coordinated reaction pathways that capture free energy present in light to yield ATP and NADPH, which power the production of organic molecules. a. During photosynthesis, chlorophylls absorb free energy from light, boosting electrons to a higher energy level in Photosystems I and II. b. Photosystems I and II are embedded in the internal membranes of chloroplasts (thylakoids) and are connected by the transfer of higher energy electrons through the electron transport chain (ETC). c. When electrons are transferred between molecules in a sequence of reactions as they pass through the ETC, an electrochemical gradient of hydrogen ions (protons) across the thylakoid membrane is established. d. The formation of the proton gradient is a separate process, but it is linked to the synthesis of ATP from ADP and inorganic phosphate via ATP synthase. e. The energy captured in the light reactions as ATP and NADPH powers the production of carbohydrates from carbon dioxide in the Calvin cycle, which occurs in the stroma of the chloroplast. 7. I can explain how the electron transport chain captures free energy from electrons in a series of coupled reactions that Videos: Bozeman: Photosynthesis Crash course: Photosynthesis Prezi: Photosynthesis establish an electrochemical gradient across membranes. a. Electron transport chain reactions occur in chloroplasts (photosynthesis). b. In photosynthesis, the terminal electron acceptor is NADP+. c. The passage of electrons is accompanied by the formation of a proton gradient across the thylakoid membrane of chloroplasts with the membrane(s) separating a region of high proton concentration from a region of low proton concentration. Monday January 18 Tuesday January 19 No School – MLK Day Ch. 10 one-pager due tomorrow Photosynthesis (POGIL) Modeling Photosynthesis Activity Pre-Lab AP Lab 5 Photosynthesis Summary Table Pre-Lab Quiz Mastering Biology Chapter 10 quiz by 11:59pm Lab poster for studentdesigned experiment to be presented by end of class tomorrow See Unit 6 syllabus AP Lab 6 Due! Calvin Cycle Wednesday January 20 Thursday January 21 AP Lab 5 – Guided Inquiry & Student Design Friday January 22 Quiz – ch. 10 Work on, present AP Lab 5 posters Monday January 25 Bioflix – Photosynthesis Walk through PS cards Review Tuesday January 26 Unit 5 Test