Newman samples for Ion Torrent Run
The samples are those run in lane 11 of each gel (3 uL of sample).
Top gel = Lycomia haifense,; Bottom gel = Kaistella koreensis
I would estimate their concentration at ~200 ng/uL
The tubes (to be) enclosed contain 25 µL of these samples (~5 µg total) I hope this is OK.
THANK YOU!
Figure 4. Genomic DNA gel. Isolation of genomic
DNA was performed using Qiagen Genomic DNA
Purification. The genomic DNA was isolated from four
different organisms. The wells contained a first or
second elution of the following: lanes 1, 2, and 3
contained uncut γ DNA at concentrations of 20ng/µL,
50ng/µL, and 80ng/µL; lane 4 contained a 10 µL 2 log
ladder, as a marker; lanes 5 and 6 contained the first
and second elutions of Micrococcus.luteus LYG1,
respectively; lanes 7 and 8 contained the first and
second elutions of Micrococcus. luteus LYE1,
respectively; lanes 9 and 10 contained the first and
second elutions of Chryseobacterium novum JM1,
respectively; and lanes 11 and 12 contained first and
second elutions of Lycomia haifense, respectively.
Figure 2. Genomic DNA gel The genomic DNA in
lanes 1-12 were, respectively, 20 ng/uL uncut DNA,
50 ng/uL uncut DNA, 80ng/uL uncut DNA, 2 log
ladder (1000ng total), 1st elution Chryseoindolgenes
FH3, 2nd elution FH3, 1st elution Micrococcus
yunnanensis DSM, 2nd elution Micrococcus
yunnanensis DSM, 1st elution Micrococcus luteus
LYO1, 2nd elution Micrococcus luteus LYO1, 1st
elution Kaistella koreensis, and 2nd elution Kaistella
koreensis.