Supplementary Table 6: S. japonicum antigens on the schistosome protein microarray associated with rodent infection. Antigens were
selected by Bolm-Hochberg adjusted p-values between pre-infection and post-infection samples from rat time course one (TC1) and two (TC2),
mouse (M) and ASC (A) sample probing.
Accession
Number
ID
Annotation (BLASTp % amino
acid
identity)$
AF048759
Sj21.7 - 21.7 kDa antigen (65% aa
17PD36
Sm21.7 Smp_086480)
AY815164
18PD38
protein (77% aa Sm heat shock
33PD74
Smp_026570)
AY815140
Sj Annexin 7 (47% aa Sm putative
35PD78
annexin CCD80878)
AY808751
149PD215
antigen (84% aa Sm TAL12
Peptide#
transmem.
Domains#
Lifecycle
Tegument
Process
Stage#
Surface#
Gene Ontology
Details
(GO)#
Microtubule-
Sj Sm vaccine; EF-hand
based
family1,2
y
Protein folding
Few studies3
-
n
-
1
C
n
-
n
-
-
n
36
n
-
-
n
-
TC1
355
n
-
CSAEM
y
-
TC1 TC2 A
191
n
-
-
y
TC1 TC2 A
185
n
-
CSAEM
y
TC1
166
n
-
CSAE
TC2
185
n
1
TC1
375
n
TC1 TC2
187
TC1
Smp_045010);
Hypothetical protein
Sjp40, p40 major egg antigen
157PD223
(83% aa Sm p40 CCD76213)
162PD228
(aa)
Signal
SjTAL12, sim. Sm22.6 tegument
AY814158
L08198
Length
Biological
86)
sim AAP05970 (62% aa Sm innexin
121PD183
Sample
Number of
SjHSP86, sim. 86 kDa heat shock
AY811126
AY815113
Protein
Sj22.6 - 22.6 kDa tegumental
associated antigen (72% aa
Sm22.6 / 9CF)
Microtubulebased
Limited information, signal
peptide
Putative vaccine target4,5
Sim. to Sj22.6 (48% aa)
Limited information
Anti-pathology Sm
vaccine6,7
Microtubule-
Sj Sm vaccine; EF-hand /
based
dynein light family8,9
Accession
Number
ID
Annotation (BLASTp % amino
acid
identity)$
AY222926
hypothetical protein with IG
164PD230
domain
AF072327
167PD235
M14654
169PD237
Domains#
Lifecycle
Tegument
Process
Stage#
Surface#
Gene Ontology
Dynein light chain 1
TC1 TC2 A
89
n
-
-
n
put. SjGST 26 antigen
TC1 TC2
218
n
-
-
TC2
162
n
-
TC1 TC2
125
y
1
Sj-L6L-1, sim. ThB / CD59
184PD254
aa Cs calponin-3)
M63706
Sj23 23 kDa integral membrane
185PD255
prot. / CD63
-
242
n
-
Limited information, signal
peptide10
Microtubule-
Sj Sm vaccine ; dynein light
based
family11,12
y
-
Sj Sm vaccine13,14
E
n
-
Limited information
AM
n
Actomyosin
TC1 TC2 A
Details
(GO)#
y
Calponin-like; sim. calponin (87%
212PD289
transmem.
-
AY813467
AY223465
Peptide#
1
family
187PD257
(aa)
Signal
y
177PD246
AF380366
Length
Biological
291
sim. EAA15991 prot; knottin fold
179PD248
Sample
Number of
TC1
AY812161
AY816003
Protein
CSA
y
structure
organization
M
218
n
4
SA
n
-
TC1
400
n
1
A
y
-
TC1
420
n
-
SAE
n
-
Potential vaccine
candidate13
Homologous to a Sj
vaccine13,14
Sj Sm vaccine, tetraspanin
family15
Serine protease inhibitor serpin
B6 (SjB6) / oestrogen-regulated
Sj Sm vaccine16,17
protein
hypothetical protein / Sj
phosphatase in vivo recombinant
prot.
Limited vaccine protection,
serum reactivity†18
$
S. mansoni (Sm) or Cloronchis sinensis (Cs) BLASTp identity with expected value < 10-50; # Protein lifecycle stage expression for cercaria (C),
schistosomulum (S), adult (A), egg (E) and miracidium (M); detected on tegument surface by proteomics 19; † (Zhang, Li, McManus,
unpublished data).
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