(NSF ATE Award #0903208)
(NSF DUE ATE Award #1003499)
Protein is Cash: Introduction to Biomanufacturing Teachers Workshop
Cooper Campus
August 15th to August 18th
Chief Contact: Linnea Fletcher, Ph.D. (linneaf@austincc.edu)
Secondary Contact: Steven Spurlock (sspulock@austincc.edu)
Day of Workshop
Time
Material Covered
Location
Day 1:
August 15th
9-10am
Overview of Discovery Research and
Upstream Processing – Sonia Wallman
Coffee and Snacks
Industry Speaker: (Marianna Goldrick, BIOO Scientific)
RM 9109
10am-12pm
Bacterial Transformation and
Upstream Processing – Mary Jane Kurtz
RM 9109
12-1pm
Lunch & Bio-Link Networking Linnea Fletcher RM 9109
1-4pm
Transformation and Upstream Processing
RM 9101
-UV Lamp
-Timer
-Microwave Oven
-37C Incubator
-42C Water Bath
-Glassware: 1L flask,
500ml graduated cylinder
-Distilled Water, 500ml
-Crushed Ice and containers
-Sharpies
Day of Workshop
Time
Cont. 1-4pm
Material Covered
Location
Transformation and Upstream Processing
Provided in Bio-Rad ‘pGLO
Bacterial Transformation’ Kit:
-Escherichia coli HB101 K-12, lyophilized
-Plasmid (pGLO), Lyophilized, 20ug
-Ampicillin, lyophilized, 30mg
-L(+) Arabinose, lyophilized, 600mg
-Transformation solution (50mMCaCl2,
pH6.1, sterile, 15ml)
-LB nutrient broth, sterile, 10ml
-LB nutrient agar powder, sterile (to
make 500ml)
-Pipettes, sterile
-Inoculation loops, sterile, 10ul
-Petri dishes, 60mm
-Microtubes, 2.0ml and foam micro test
tube holders
Day 2:
August 16th
9-10am
Introduction to Downstream Processing
and Chromatography – Sonia Wallman
Coffee and Snacks
RM 9109
10am-12pm
Chromatography – Mary Jane Kurtz
RM 9109
12-1pm
Lunch & Bio-Link Networking continued
RM 9109
2-4pm
Manufacturing Tour – Life Technologies
Drive or Ride
Day of Workshop
Time
Material Covered
Location
Day 3:
August 17th
9-10am
Overview of Biofuels – Sonia Wallman
10am-12pm
Cellulosic Biofuels Lab – Mary Jane Kurtz
RM 9109
12-1pm
Lunch and Networking
RM 9109
1-4pm
Cellulosic Biofuels Lab Cont.
RM 9101
-Safety Glasses or goggles
-Microfuge tubes
-Indelible markers
-Power supplies for
SDS-PAGE gel Boxes, 0-200 Volts
-Pipettors 20-200ul
-Pipette tips
-Boiling water bath
-Test tubes (5ml) 100
-Staining Tray
-Cuvettes
-Pipettes 5ml
-Pipet bulbs or automatic pipettor
Provided by Bio-Rad:
-SDS-PAGE Gel Boxes
-Pre-poured SDS-PAGE Gels
-Running Buffer
-Laemmeli Buffer
-Protein Ladder
-Protein Standards
-Coomassie Blue Stain
Day of Workshop
Day 4:
August 18th
Time
9-11am
Material Covered
Introduction to Sampling and Quality Control
Biochemistry – Sonia Wallman
Coffee and Snacks
Location
RM 9109
11am-12pm
Chromatography – Mary Jane Kurtz
RM 9109
12-1pm
Lunch and Networking
RM 9109
1-4pm
Chromatography
RM 9101
- Safety Glasses or Goggles
- Microtubes
- Microtube rack
- Pipettor Sets (20ul, 20ul, & 1000ul)
- Sharpie
- E. coli GFP cell culture
(from Day 2)
-Freezer
- Microfuge centrifuge
- Vortexer
- TE Buffer
- Lysozyme in 1ml of TE Buffer
- UV Lamp
- Stand to hold chromatography
Column
- Test tubes (5ml volume)
- Test tube rack
- Disposable pipettes~3ml
- UV black light
- Stir plate with magnetic stir bar
-Waste beaker
Day of Workshop
Time
Cont. 1-4pm
Material Covered
Location
Chromatography
RM 9101
Provided in Bio-Rad ‘Green Fluorescent
Protein Chromatography’ KIT:
-Binding buffer
-Column equilibration buffer
-Column wash buffer
-Elution buffer
-HIC chromatography columns
-Disposable plastic transfer pipettes
-Micro test tubes, 2.0ml, clear
-Sample collection tubes, 5m;
Provided by NBC2:
- Chromatography Column Buffers:
+ Gel Filtration Column Buffer
(=0.15M NaCl solution)
+ Anion Exchange
Chromatography Buffers:
Equilibration buffer (=50mM
Tris pH 8.3); Elution buffer 1 (50 mM
Tris pH 8.3, 130mM NaCl); Elution
buffer 2 (50 mM Tris pH 8.3, 200mM
NaCl); Elution buffer 3 (50 mM Tris pH
8.3, 300mM NaCl)
-Columns for Gel Filtration
Chromatography 25cmx2.0cm
-Sephadex G-75 Gel filtration Medium 10
grams
-Pre-packed MacroPrep High Q for
Anion Exchange Column