1
Supplemental Table 1. Concentrations of TNT freshly amended into test soils used in definitive
toxicity tests with Enchytraeus crypticus.
Nominal
Determined
Determined/
Concentration
Concentration
Nominal
(mg/kg)
(mg/kg)
(%)
Teller sandy loam
0
BDL
NA
10
4(0.1)
40
60
49(1.0)
82
80
69(0.7)
86
100
88(1.3)
88
120
109(1.6)
91
Sassafras sandy loam †
0
BDL
NA
50
40(0.4)
80
75
62(2.1)
82
100
85(0.2)
85
150
134(6.1)
90
200
186(2.4)
93
300
287(2.5)
96
Kirkland clay loam
0
BDL
NA
2
50
41(0.3)
82
100
88(1)
88
150
132(4)
88
200
180(7)
90
250
224(9)
90
300
300(10)
100
350
380(24)
108
500
527(15)
105
800
840(20)
105
Richfield clay loam
0
BDL
NA
100
80(2)
80
200
168(2)
84
300
267(6)
89
400
330(2)
83
600
538(6)
90
1000
900(13)
90
Webster clay loam
0
BDL
NA
150
86(9)
57
250
245(11)
98
300
284(17)
95
400
387(6)
97
3
450
433(7)
96
600
564(21)
94
700
670(30)
96
800
835(17)
104
900
890(46)
99
Analytically determined concentrations, shown as means and SEs (parentheses), n = 3, are based
on acetonitrile extraction (USEPA Method 8330A) of TNT from soil.
NA = not applicable.
BDL = below detection limit (0.05 mg/L in solution, and 0.5 mg/kg in soil).
†
Modified from Kuperman et al. [11]; included in this table for convenience of comparison with
other soils reported herein.
4
Supplemental Table 2. Concentrations of TNT weathered-and-aged (W-A) in test soils used in
definitive toxicity tests with Enchytraeus crypticus.
Nominal
Initial
W-A
W-A /Initial
Concentration
Determined
Determined
Determined
(mg/kg)
(mg/kg)
(mg/kg)
(%)
Teller sandy loam
0
BDL
BDL
NA
20
17 (4)
2.5 (0.1)
15
50
52 (5)
27 (0.7)
52
100
54 (4)
42 (1.5)
78
160
181 (13)
135 (2)
75
200
229 (14)
175 (2)
76
225
268 (15)
191 (3)
71
275
328 (20)
233 (2)
71
350
370 (14)
289 (2)
78
Sassafras sandy loam †
0
BDL
BDL
NA
20
18 (0.2)
3 (0.5)
17
75
73 (0.8)
46 (0.7)
63
100
92 (0.8)
66 (2.5)
72
150
139 (5.3)
94 (0.0)
68
160
150 (0.6)
105 (2.7)
70
5
180
175 (8.6)
137 (3.8)
78
Kirkland clay loam
0
BDL
BDL
NA
100
88 (1)
2 (0.1)
2
150
132 (4)
12 (0.3)
9
250
224 (9)
26 (6.5)
12
300
300 (10)
147 (1.6)
49
350
380 (24)
147 (4.3)
39
500
527 (15)
313 (7.7)
59
800
840 (20)
553 (6.9)
66
Richfield clay loam
0
BDL
BDL
NA
300
267 (6)
35 (1)
13
600
538 (6)
93 (2)
17
700
268 (8)
142 (4)
53
800
708 (24)
253 (3)
36
1000
900 (13)
391 (8)
43
Webster clay loam
0
BDL
BDL
NA
150
86 (9)
7 (0.3)
8
300
284 (17)
30 (0.3)
11
500
522 (23)
165 (2.3)
32
600
564 (21)
182 (1.9)
32
6
700
670 (30)
528 (25)
79
900
890 (46)
695 (11)
78
Analytically determined concentrations, shown as means and SEs (parentheses), n = 3, are based
on acetonitrile extraction (USEPA Method 8330A) of TNT from soil.
NA = not applicable.
BDL = below detection limit (0.05 mg/L in solution, and 0.5 mg/kg in soil).
†
Modified from Kuperman et al. [11]; included in this table for convenience of comparison with
other soils reported herein.
7
Supplemental Table 3. Concentrations of RDX freshly amended (FA) into test soils or
weathered-and-aged (W-A) in test soils used in definitive toxicity tests with Enchytraeus
crypticus
Nominal
FA
W-A
Concentration
Determined
Determined
(mg/kg)
(mg/kg)
(mg/kg)
Teller sandy loam
0
BDL
BDL
1000
1200(260)
1060(63)
2000
2200(73)
2300(60)
4000
4500(700)
5560(564)
5000
6300(715)
7000(1300)
10,000
10,000(787)
15,000(1100)
Sassafras sandy loam†
0
BDL
BDL
300
300(19)
NT
600
660(19)
NT
1200
1200(22)
1050(20)
2400
2200(72)
2380(41)
4800
4560(140)
3985(43)
10,000
10,000(370)
9550(370)
20,000
21,400(1200)
18,350(520)
8
Analytically determined concentrations, shown as means and SEs (parentheses), n = 3, are based
on acetonitrile extraction (USEPA Method 8330A) of RDX from soil.
NT=Not Tested in the study.
BDL = below detection limit (0.05 mg/L in solution, and 0.5 mg/kg in soil).
†
Modified from Kuperman et al. [10]; included in this table for convenience of comparison with
other soils.