IHS / IB Biology 2 25 Questions KEY! 1. One can apply a two-tailed t-test to analyze data from an experiment if the data are normally distributed (think: bellshaped curve) and if N is at least 10. (You won’t be expected to calculate t, but you will need to use a t-value and statistical tables to determine if two sets of data (e.g. control and experimental) are significantly different. 2. About 68% of all the data in a set fall within one standard deviation, if the data are normally distributed. Remember, you want to put the standard deviation of the sample (s) button, not the total population’s standard deviation (sigma button), on your calculator. 3. Roles for a. Sulfur: part of some amino acids b. Calcium: muscle contraction, neurotransmitter exocytosis in animals c. Phosphorus: DNA, RNA, ATP d. Iron: hemoglobin in animals e. Sodium: Na/K pump, Na channels in excitable animal cells 4. A condensation reaction between two amino acids pulls the H from the amino group of one a.a. and the OH from the carboxyl group of the other a.a. and forms water, in the process linking the amino acids with a covalent bond called a peptide bond. 5. Lactose free milk is produced by pre-treating milk (containing lactose disaccharide) with lactase, the enzyme that hydrolyzes lactose into glucose and galactose. People who are lactose intolerant (whose bodies have stopped producing lactase) can then consume the lactose free milk without developing tummy troubles. The lactase to pretreat the milk is a product of biotechnology (the gene for lactase was transferred to cells in culture). 6. Four proteins’ names and jobs: a. Hemoglobin – carries oxygen within blood cells b. Amylase – digests starch, made by salivary glands and pancreas c. Silk – structural protein in spider webs d. Collagen – structural protein in hair, nails 7. Cells are so small because it maximizes their surface area to volume ratio. The rates of exchange of food and wastes is a function of a cell’s surface area, while the rates of food consumption and waste production are a function of a cell’s volume. Examples of a. Competitive inhibition (inhibitor resembles substrate and competes for active site): Example - Ethanol is oxidized in the body to acetaldehyde, which is in turn further oxidized to acetic acid by aldehyde oxidase. Normally, the second reaction is rapid so that acetaldehyde does not accumulate in the body. A drug, disulfiram (Antabuse) inhibits the aldehyde oxidase, which causes the accumulation of acetaldehyde with subsequent unpleasant side effects (nausea, vomiting). This drug is sometimes used to help 8. people overcome a drinking habit. b. Noncompetitive inhibition (inhibitor does not resemble substrate; binds elsewhere on enzyme but in so doing still affects shape of active site): Compounds containing heavy metals such as lead, mercury, copper or silver are poisonous. This is because ions of these metals are noncompetitive inhibitors for several enzymes. For instance, silver ions react with –SH groups in the side groups of the amino acid cysteine in a protein chain. 9. Labeled prokaryote drawing should include a cell wall, plasma membrane, cytoplasm, pili, flagella, ribosomes, and a region of cytoplasm containing the prokaryote’s DNA (the nucleoid). Prokaryotes tend to be spherical, rod-shaped, or spiral-shaped, so that could be included. 10. In interphase, there are three different sets of activities in cells. In the first part of interphase, G-1, cells are synthesizing proteins and carrying out all their moment-tomoment metabolic reactions. In the middle of interphase, S, cells replicate their DNA. That’s all they do! In the third portion of interphase, G-2, which is typically fairly short, cells resume protein synthesis, especially for proteins that would be needed in cell division (like tubulin, which is part of microtubules). Also during interphase the cells’ organelles must be roughly doubled. Mitochondria and chloroplasts divide on their own during interphase. 11. Chlorophyll molecules’ structure enables them to absorb red and blue, primarily. They do not absorb green. Green is reflected, giving plants their green color. We would be aware of these differences in absorption by inspecting the absorption spectrum of chlorophyll. Memorize: wavelength of blue light about 400 nm, wavelength of green light about 550 nm, wavelength of red light about 700 nm. 12. A nucleosome is a bead of 8 histone proteins on which DNA is “wound.” Nucleosomes help “package” a large amount of DNA into a tiny space, the nucleus (eukaryotic cells). There is a ninth kind of histone found between nucleosomes. While a gene is being transcribed, it is temporarily loosened or released from its nucleosomes. 13. Transfer RNA (tRNA) molecules have a three-dimensional, “L” shape. Although fundamentally they are a string of nucleotides, they coil up and internally base pair. The shape of a tRNA is recognized by a tRNA-activating enzyme, which puts amino acids on the top of their corresponding tRNA molecules. The sequence CCA appears at the 3’ end, the end at which the appropriate amino acid is attached to the ribose of the last adenine – in red on picture). The loop with the anticodon is at the opposite end of the L shape, green in picture. tRNAs are re-used. 14. Cloning for therapeutic purposes (creating cells to replace a patient’s diseased cells) involves isolating a nucleus from one of the patient’s differentiated cells (like a skin cell), putting it into an egg cell from which the nucleus has been removed, and getting the egg to start dividing, often with a mild electrical shock. As the egg-and-diploid nucleus “embryo” divides, it therefore produces a bank of cells that could be chemically treated to differentiate in a certain way to cause them to differentiate into the cell type needed (heart, brain, etc.), and then given to the patient. 15. An energy pyramid is pyramid-shaped because it is made by stacking the energy available to successive trophic levels (TL), with producers on the bottom and carnivores at the top. Since only 10% of the energy available to one TL becomes available to the next TL, the stack sharply narrows. Photosynthesis captures only a fraction of the sun’s energy; respiration is less than 50% efficient; and, energy is expended to catch and eat prey. Units of an energy pyramid typically = kJ or kCal per m2 per year. 16. How global temperature rise is impacting the arctic (way more than just four consequences!) a. It is causing ice melt, which deprives animals like polar bears of places to hunt/rest b. It is causing permafrost to thaw, which increases decomposition rate of previously-frozen detritus, thereby releasing more CO2 to the atmosphere c. By melting ice, it is causing sea levels to rise, displacing people and wildlife d. Some temperate zone species like grizzlies are expanding their ranges northward 17. An S shaped population growth curve has 3 phases: exponential or steep initial phase, transitional phase, and leveling off at K or carrying capacity, the plateau phase. 18. An apical meristem is perpetually embryonic (ie mitotic) tissue at the tips of roots and shoots, increasing their length, while a lateral meristem is perpetually embryonic tissue around the sides of a shoot or root, increasing their girth. The vascular cambium is a lateral meristem. 19. Abscissic acid or ABA promotes the closure of a plant’s stomata. It is synthesized when a plant is experiencing drought stress. 20. Phytochrome comes in two shapes, Pr and Pfr. Pr is synthesized from scratch. In sunlight (which includes red light), Pr is converted to Pfr. In darkness, Pfr slowly reverts to Pr. Pfr promotes flowering in long-day (short night) plants, and inhibits flowering in short-day (long night) plants. 21. Cells’ jobs and locations: a. Blastocyst cells (aka blastomeres) are undifferentiated (unspecialized) cells of an embryo. As development proceeds, blastocyst cells divide and divide – their progency become differentiated as muscle cells, brain cells, etc. b. Leydig cells (aka interstitial cells) produce and secrete testosterone. They are located in the spaces between seminiferous tubules in the testes. (“Interstices” are crevices.) Leydig cells respond to commands from LH, secreted by the pituitary. c. Polar bodies are haploid cells produced during meiosis of a primary oocyte. Their job is to remove excess chromosomes so that the final egg cell (in humans) has 23, unreplicated chromosomes. They are found in the ovary. d. Sertoli cells are the cells that nourish developing spermatogonia all the way to spermatozoans. They are stimulated by FSH, secreted by the pituitary. 22. Crossbridges are formed by the heads of myosin molecules, which as a group constitute a thick filament in the myofibril of a muscle fiber. They attach to exposed actin molecules (on thin filaments) when calcium presence has removed a blocking molecule away from the thin filaments. (When a nerve impulse tells a muscle fiber to contract, calcium is released within the fiber.) By spending ATP to detach and re-set the crossbridges, which bend like rowing boat oars, thick filaments slide between thin filaments and contraction (shortening) of the fibers occurs. 23. In contralateral processing of visual stimuli, the “wiring” from the retinas of both eyes to the occipital lobes is such that light received by the left visual field is projected to the right visual cortex, while the light received by the right visual field is projected to the left visual cortex. (It is NOT true that light received by the left eye goes to the right visual cortex and vice versa. Both eyes’ neural impulses go to both the left and right visual cortices.) 24. One effect of THC on synapses in the brain is to inhibit neurons that were inhibiting VTA neurons. As a result, there is more dopamine in the nucleus accumbens (the pleasure or reward center of the brain). THC mimics naturally occurring endocannabinoids that, when binding to receptors, affect appetite, pain sensation, mood, and memory. 25. Bluegills (a type of sunfish) forage optimally, as it turns out, for prey like Daphnia. Daphnia are small arthropods related to crabs and lobsters. In a famous series of experiments conducted in the 1970’s, scientists discovered that, in low density prey conditions, bluegills take small, medium, and large Daphnia. However, when prey is abundant, bluegills spend the extra search time to forage for and consume mostly large Daphnia. See figure 9.4 below.