Bruker’s New TopSolids™ Software Provides Accelerated Workflow in Solid-State NMR for
the Characterization of Protein Structures
Automated spectrometer adjustment together with a comprehensive range of pre-defined protocols guarantees
optimum spectral quality and high productivity for both expert and novice users
CHICAGO, Illinois – March 3, 2014 – At Pittcon 2014, Bruker today introduced TopSolids™, a new intuitive,
workflow-based software package for solid-state NMR in structural biology that provides menu-based, automated setup and
acquisition of the most advanced multinuclear multi-dimensional NMR experiments. TopSolids provides a comprehensive
library of protocols for structural biology, and ensures robust, secure spectrometer and probe setup. The combination of
optimized acquisition parameters with fine tuning on the actual research sample guarantees unsurpassed spectral quality.
Never before has biological solid-state NMR, one of the most important tools for understanding the structure and dynamics
of complex insoluble protein systems, been so readily accessible to users from a broad diversity of background and
experience.
TopSolids encompasses a library of multidimensional
experiments, as well as the complete set of semi-automated experimental
preparation steps, including guided magic angle adjustment and automated
shimming. Based on recognized rf-probe efficiencies, a fully automatic
measurement of 1H and 13C rf-pulses is rapidly realized by a novel
proprietary method. Automated pulse calibration is followed by finetuning of 1H, 13C and 15N pulses on the actual sample. These steps ensure
correct parameter adjustment for the potentially differing conditions of wet and dielectric samples, as compared to the
typically dry setup sample. All multidimensional experiments can be queued and combined with non-uniform sampling
techniques for maximum productivity and a flexible, efficient lab workflow. While TopSolids gives the casual solid state
NMR user access to the most sophisticated solid-state NMR experiments, guaranteeing the highest quality results, the
expert user will benefit from interactive customization of pulse lengths, power levels, shapes, decoupling sequences and
strengths at any time during the experiment setup procedure. Upon completion of the experiments a final PDF report is
generated, providing a summary of all the important parameter settings, assisting with fast publication.
“TopSolids will both ease new entrants to the often considered complex and time-intensive research field of
Solid-State NMR spectroscopy, while equally providing experienced method developers with significantly accelerated
workflow through assisted spectrometer setup, semi-automated yet customizable calibration and an unmatched choice of
multidimensional protocols. It simply has never been easier to take up solid state NMR spectroscopy and seriously boost
your productivity and spectral quality”, commented Jochem Struppe and Sebastian Wegner, product managers for SolidState NMR at Bruker BioSpin.
Part of the TopSpin software family, Bruker’s acknowledged industry-standard for NMR acquisition and
processing, TopSolids is fully compatible with Windows and Linux operation systems, and seamlessly integrates with
Bruker AVANCE™ III and III HD spectrometers. The combination of TopSolids and Bruker’s Efree TM probe technology
provides complete peace of mind for the Biochemist working with wet protein samples, with or without phospholipids.
Optimized parameters for these experiments are included, as are standard parameters for effective control of any rf-heating
of samples.
About Bruker Corporation
Bruker Corporation (NASDAQ: BRKR) is a leading provider of high performance scientific instruments and solutions for
molecular and materials research, as well as for diagnostics, industrial, clinical and applied analysis.
For more information, please visit www.bruker.com
For more information on TopSolids, please visit: www.bruker.com/topsolids
Media Contact:
Dr. Thorsten Thiel
Bruker Director of Marketing Communications
T: +49 (721) 5161 – 6500
E: thorsten.thiel@bruker.com