Assistant Professor (TTS)
Biosciences Department,
COMSATS Institute of Information Technology, Park
Road Chak Shehzad, Islamabad- 44000, Pakistan.
Jhelum-Punjab (Pakistan)
House # 38 A-Block Satellite Town,
Rawalpindi--Pakistan
<saleem.a.bokhari@comsats.edu.pk>
+92 (0)333 516 9415
Designation:
Mailing Address:
Domicile:
Present Address:
E-mail:
Contact No.:
Biochemistry/Molecular biology
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Ph.D., Biology (2008) Tsinghua University, Be ijing, P. R. China.
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M.Phil., Biochemistry/Molecular Biology (2000) Quaid-i-Azam University .
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M.Sc., Chemistry (1990) Government College, Lahore.
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B.Sc., Botany, Zoology, Chemistry (1986) Govt. Gordon College Rawalpindi.
Academic:
 Teaching “Protein Chemistry” to MS students/ Biochemistry courses to BS students.
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Teaching MS Biochemistry course ‘Enzyme Catalysis’.
Administrative:
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Member Vigilance Committee for the conduct of terminal examinations, Fall 2012 and spring 2013.
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Member Department Committee to conduct performance audit.
Technical Know-how
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Techniques involved in 2-D gel electrophoresis based Proteomics work-flow
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Gel electrophoresis (Native-PAGE, SDS-PAGE, SDS-DR-PAGE), PCR, protein purification techniques, Fast Protein Liquid Chromatography (FPLC), HPLC and
Gas Chromatography (GC)
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Basic techniques of biochemistry /molecular biology and microbiology
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Enzyme characterization, kinetics / thermodynamics and thermostability studies.
Research Interests
My diverse experience with a background of Chemistry and Biochemistry makes me suitable to undertake interdisciplinary studies in the field of Biosciences. I have strong predilection for applied research besides basic research.

Basic:
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Stress Response Proteomics studies
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Purification and characterization of important recombinant enzymes/proteins
Applied:
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Investigation of effect of a herbal formulation on Hepatitis C infected patients
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Biogas production by using different substrates
Research Plans:
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A ~16 M Rs. project submitted to HEC titled : “A proteomic exploration of effect of strigolactones on regulation of tillering in rice”, as CoPI, is currently under review.
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A research project in collaboration with Institute of Biomedicine and Genetic
Engineering, Islamabad, titled : “Verifying effectiveness of a herbal medicine against Hepatitis C virus infection”, of worth 5.8 M Rs. is submitted to HEC for evaluation.
 A collaborative Research project: “Validating biochemical basis of efficacy of a herbal formulation against hepatitis C virus infection”, is under preparation with
Prof. Khalid Rehman of Liverpool John Moore University, UK under Pakistan
Program for Collaborative Research (PPCR) of HEC.
Previous Responsibilities:
Teaching:
 Taught ‘Proteomics’ to PhD students.
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Taught biochemistry-I & -II courses at graduate and undergraduate level.
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Designed MS Forensic Chemistry and developed curriculum and courses for the entire program-first of its kind in the country
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Taught ‘Drugs of Abuse’, ‘DNA Analysis and Serology’ and ‘Separation
Techniques courses ’ to MS Forensic Chemistry students
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Teaching experience at undergraduate level (FSc/BSc) since 1992 to 2008.
Administrative:
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Program Coordinator MS Forensic Chemistry till Dec 2011
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Incharge department library
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Incharge Forensic Chemistry Lab at GCU Lahore
Research:
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Supervised 4 BS and 4 MS Students Forensic and Biochemistry on the topics such as: detection of Benzodiapines in hair of Narcotics addicts, determination of antioxidant, antimicrobial and hepato-protective activities of herbal extracts and effect of microwave irradiation on antioxidant potential.
Employment History

Post-Phd:
April 2010 to date: Biosciences Department CIIT, Islamabad
2009 to 2012: GC University, Lahore, as Assistant Professor
Pre-Phd:
1994-2008: Education Department, Govt. of Punjab as Lecturer
1992-1994: Sir Syed Science College, Aligarh Old Boys Association,
Rawalpindi, as Lecturer Chemistry
1991-1992: NIBGE/NIAB Faisalabad, as Trainee Research Fellow
Personal highlights
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HEC approved Ph.D. supervisor
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Designed M.S. Forensic Chemistry program, first of its kind in the country, developed curriculum and course contents which was duly endorsed by Punjab
Forensic Science Agency (PFSA), Lahore
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Drafted MoUs for collaboration with Punjab Anti Narcotic Force (ANF) and PFSA,
Lahore
 Developed a course, ‘Separation Techniques’ for M.Phil., Chemistry program for
GCU Lahore in 2010.
 Developed a course, ‘Enzymes Catalysis’ for MS Biochemistry/Molecular Biology program (to be offered in spring 2014) for CIIT, Islamabad.
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PhD Scholarship for Basic Sciences in China. Higher Education Commission of
Pakistan 2004 Program
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PhD from the top universities of the China — Tsinghua University .
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Won cash award in Tsinghua University for publishing in a journal of impact factor above 5
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Worked as Trainee Research Fellow in NIAB and NIBGE, (1991-92) and completed research work of my M.Phil., at NIBGE (1998-99)
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Refereed research papers for Journal of Experimental Botany (Impact Factor 3.8).
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Academic Roll of Honor, Government College Lahore, in M.Sc.
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Awarded of Trainee Research Fellowship (1991-92) in National Institute of
Biotechnology and Genetic Engineering (NIBGE) / Nuclear Institute of Agriculture and Biology (NIAB), Faisalabad.

President Talent Farming Scheme Scholarship in B.Sc.
Research Topics o Ph.D: “
2.
” o
M.Phil:
“
o

1. Bokhari, S. A .; Wan, X-Y.; Yang, Y-W.; Zhou, L.; Tang, W-L.; Liu, J-Y. (2007);
Proteomic response of rice seedling leaves to elevated CO
2
levels. J. Proteome
Res., 6 (12), 4624-4633. IF: 5.460
2. Zhou, L., Bokhari, S. A ., Dong, C-J, Liu, J-Y. (2011). Comparative proteomics analysis of the root apoplasts of rice seedlings in response to hydrogen peroxide.
PLoS One, 6 (2) e16732 (open access). IF: 4.411
3. Afzal, A.J., Bokhari, S.A
. Siddiqui, K.S. (2007) Kinetic and thermodynamic study of a chemically modified highly active xylanase from Scopulariopsis sp: existence of an essential amino group. Appl Biochem Biotechnol. 141(2-3):273-97. IF:
1.879
4. Siddiqui, K.S., Bokhari S.A
., Afzal, A.J., Singh S. (2004).A novel thermodynamic relationship based on Kramers Theory for studying enzyme kinetics under high viscosity. IUBMB Life, 56 (7) 403-407. IF: 4.251
5. Bokhari, S.A
., Afzal, A.J., Rashid, M.H. Rajoka, M. I., Siddiqui, K.S. (2002).
Coupling of surface carboxyls of carboxymethylcellulase with aniline via chemical modification: Extreme thermostabilization in aqueous and water-miscible organic mixtures. Biotechnol. Prog. 18 (2) 276-281. IF: 2.178
6. Afzal, A. J., Bokhari, S. A ., Ahmed, W., Rashid, M. H., Rajoka, M.I. Siddiqui,
K.S. (2000). Two rapid and simple methods for the detection of polymer degrading enzymes on high resolution, alkaline, cold, in situ-native (HiRACIN)-
PAGE and high resolution, in situ inhibited native (HiRISIN)-PAGE. Biotechnol.
Lett. 22 (11) 957-960. IF: 1.768
7. Abbasi, B.H., Khan, M., Guo, B., Bokhari, S. A ., Khan, M.A. (2011). Efficient regeneration and antioxidative enzyme activities in Brassica rapa var. turnip.
Plant Cell Tiss Organ Cult. 105:337 –344. IF: 1.243
8. Tahir, M. N., Adnan, A. Bokhari, S. A.
, Jung, S. (2013). Cholesterol extraction from ghee using glass beads functionalized with beta cyclodextrin. J. Food Sci
Technol. (published on line) DOI 10.1007/s13197-013-1039-2 IF: 0.498
9. Abbasi, B. H., Ali, J., Ali, M., Zia, M., Bokhari, S. A.
, Khan, M. A. (2013). Free radical scavenging activity in in-vitro derived tissues of Eruca sativa. Toxicol. Ind.
Health (published on line) DOI: 10.1177/0748233713498450 IF: 1.423
10. Idrees M, Adnan A, Bokhari SA , Qureshi F. (2014). Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production. Brazilian J. Chem. Eng. (Gally proof accepted for 31 (2) to be published in June 2014). IF: 0.89